Biomedical Laboratory techniques

Activity type

Scienze e tecniche di laboratorio biomedico

SSD

MED/08

Year

2nd year

Semester

1st semester

CFU

1

Hours distribution

10 classroom hours

Lecturers

MANILA ANTONELLI

Activity type

Scienze e tecniche di laboratorio biomedico

SSD

MED/46

Year

2nd year

Semester

1st semester

CFU

1

Hours distribution

10 classroom hours

Lecturers

MARIA ANTONIETTA OLIVA

Activity type

Scienze e tecniche di laboratorio biomedico

SSD

MED/46

Year

2nd year

Semester

1st semester

CFU

1

Hours distribution

10 classroom hours

Lecturers

GIORGIO MANGINO

Activity type

Scienze e tecniche di laboratorio biomedico

SSD

MED/46

Year

2nd year

Semester

1st semester

CFU

1

Hours distribution

10 classroom hours

Lecturers

GIORGIO MANGINO

Activity type

Scienze e tecniche di laboratorio biomedico

SSD

BIO/12

Year

2nd year

Semester

1st semester

CFU

1

Hours distribution

10 classroom hours

Lecturers

antonietta arcella

Activity type

Scienze e tecniche di laboratorio biomedico

SSD

MED/07

Year

2nd year

Semester

1st semester

CFU

1

Hours distribution

10 classroom hours

Lecturers

antonietta arcella

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At the end of the course, students should have acquired knowledge of the topics covered


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At the end of the course, students should have acquired knowledge of the topics covered


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Students must possess basic knowledge of chemistry, biochemistry, physics and cell culture methods


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Students must possess basic knowledge of chemistry, biochemistry, physics and cell culture methods


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Awareness of the significance of the various types of biopsy: cytology,
incisional biopsy, excisional biopsy, endoscopic harvesting, surgical
removal due to the specific technical issues. Knowledge
of equipment, and tools for etching room and the pathology laboratory
Knowledge of methods of sampling and pathological staging preparations
Concept of fixation and the main types of fixative Methods for inclusion
in paraffin and resin and the various techniques Microtomic types of microtomes Principles of staining techniques basic histological and cytological. Special
stains with knowledge of the techniques of histochemistry of
carbohydrates, nucleic acids, proteins, lipids, steroids, and biogenic
amines, pigments and some minerals. Principles
of techniques of tissue criopresevazione Techniques Methods of rapid
freezing and cut with a cryostat microtome freezer Principles and
techniques extemporaneous histological examination, intraoperative
standards of hygiene and security of the law 626/92 in pathological
anatomy room Engraving: Examination of external and inside
the corpse removal of the brain and spinal cord and their variation
Evisceration organs: the chest cavity, abdominal cavity, pelvic cavity. Techniques
for the preparation of macroscopic various organs and systems in situ
and after removal and techniques for sampling and sampling of the
various organs. Techniques
autopsy of the fetus and newborn Techniques recomposition of the body
of knowledge essential Mortuary Police Regulations


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N/D
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Generalities of laboratory medicine
• Biological samples preparation and preservation
• Measure and biological variability: pre-analytical and analytical variability
• Blood and urine sampling and preservation
• Centrifugation (analytical and separative):
Differential C.
Density gradient C.
Zonal C.
Isopycnic C.
Blood cells and components isolation
Buffy Coat purification on Ficoll cushion
Immunobeads-based isolation of cell subpopulations
• Electrophoresis (horizontal and vertical E.)
SDS-PAGE
ElectroBlot
Western Blot
Cellulose acetate E.
Isoelettrofocusing
• Chromatography
Thin layer C.
Ionic exchange C.
Gel filtration C.
Affinity C.
HPLC
• Spectroscopy
Generalities
Absorbance and Transmittance - Lambert Beer Law
Spectrophotometers
Enzymatic and non-enzymatic end points measures
Turbidimetry and Nephelometry (Rayleigh’s law)
Fluorimetry
Densitometry
• Clinic Enzymology
• Immunochemistry
Generalities
Direct and indirect agglutination
Immunoprecipitation and Immunodiffusion
Immunoelelectrophoresis
Radio-Immuno Assay (RIA)
Enzyme-Linked Immuno-Sorbent Assay (ELISA)
• Automation in diagnostic



Module:
• Generalities of laboratory medicine
• Biological samples preparation and preservation
• Measure and biological variability: pre-analytical and analytical variability
• Blood and urine sampling and preservation
• Centrifugation (analytical and separative):
Differential C.
Density gradient C.
Zonal C.
Isopycnic C.
Blood cells and components isolation
Buffy Coat purification on Ficoll cushion
Immunobeads-based isolation of cell subpopulations
• Electrophoresis (horizontal and vertical E.)
SDS-PAGE
ElectroBlot
Western Blot
Cellulose acetate E.
Isoelettrofocusing
• Chromatography
Thin layer C.
Ionic exchange C.
Gel filtration C.
Affinity C.
HPLC
• Spectroscopy
Generalities
Absorbance and Transmittance - Lambert Beer Law
Spectrophotometers
Enzymatic and non-enzymatic end points measures
Turbidimetry and Nephelometry (Rayleigh’s law)
Fluorimetry
Densitometry
• Clinic Enzymology
• Immunochemistry
Generalities
Direct and indirect agglutination
Immunoprecipitation and Immunodiffusion
Immunoelelectrophoresis
Radio-Immuno Assay (RIA)
Enzyme-Linked Immuno-Sorbent Assay (ELISA)
• Flow Cytometry Fundaments
• Automation in diagnostic


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teacher's lecture notes


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Pasquinelli F.
Diagnostica e tecniche di laboratorio
Ed. Rosini Editrice
ISBN: 9788808111111


Module:
Pasquinelli F.
Diagnostica e tecniche di laboratorio
Ed. Rosini Editrice
ISBN: 9788808111111


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.ppt files distributed during the course


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The course is based on traditional lessons (two/four hours each) with the teacher teaching the whole class


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The course is based on traditional lessons (two/four hours each) with the teacher teaching the whole class


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The attendance is mandatory, and it is verified during the lessons. It is possible to sustain the final test only with an attendance of 60%


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The attendance is mandatory, and it is verified during the lessons. It is possible to sustain the final test only with an attendance of 60%


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Oral examination on topics related to the program


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The final exam is composed of an integrated test (10 queries for each subject, one correct answer each query) followed by an oral exam on the whole program (one/two questions).
The final note is the mathematical mean of the notes in each subject of the integrated teach.
To Pass the test student must obtain a note of 18/30 for each subject of the integrated teach. Student must possess a sufficient knowledge of the program.
To obtain a note of 30/30 with distinction student must possess an excellent knowledge of the whole program using a correct terminology to expose the topics


Module:
The final exam is composed of an integrated test (10 queries for each subject, one correct answer each query) followed by an oral exam on the whole program (one/two questions)
The final note is the mathematical mean of the notes in each subject of the integrated teach
To Pass the test student must obtain a note of 18/30 for each subject of the integrated teach. Student must possess a sufficient knowledge of the program
To obtain a note of 30/30 with distinction student must possess an excellent knowledge of the whole program using a correct terminology to expose the topics



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The Exam is not administred in English


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The Exam is not administred in English


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