Biomedical Laboratory techniques

Activity type

Scienze e tecniche di laboratorio biomedico

SSD

MED/08

Year

2nd year

Semester

1st semester

CFU

2

Hours distribution

20 classroom hours

Lecturers

FRANCESCA GIANNO

Activity type

Scienze e tecniche di laboratorio biomedico

SSD

MED/08

Year

2nd year

Semester

1st semester

CFU

1

Hours distribution

10 classroom hours

Lecturers

MANILA ANTONELLI

Activity type

Scienze e tecniche di laboratorio biomedico

SSD

MED/46

Year

2nd year

Semester

1st semester

CFU

1

Hours distribution

10 classroom hours

Lecturers

ANTONIO FRANCESCO CAMPESE

Activity type

Scienze e tecniche di laboratorio biomedico

SSD

MED/08

Year

2nd year

Semester

1st semester

CFU

1

Hours distribution

10 classroom hours

Lecturers

MANILA ANTONELLI

Activity type

Scienze e tecniche di laboratorio biomedico

SSD

MED/46

Year

2nd year

Semester

1st semester

CFU

1

Hours distribution

10 classroom hours

Lecturers

antonietta arcella

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Students to be able to attend classes must be in compliance with the prerequisites for the year of the course.


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General characteristics of neoplasms: nomenclature, molecular basis, metastasis, grading and staging
Atherosclerosis
Myocardial infarction and complications
Lymphomas: Hodgkin's and "non-Hodgkin's"
Acute and chronic leukemias
Myeloma
Lung Carcinoma
Colon adenoma and carcinoma
Breast cancer
Cancer of the cervix
Cytological diagnostics (PAP test)
Intracranial hypertension
Cerebral hemorrhages
Cerebral infarction
Meningitis
Demyelinating diseases
Alzheimer's disease
Parkinson's disease
Brain tumors: Gliomas, meningiomas


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cytological speciment fixation Colours Cytological classifications-Bethesda Criteria of adequacy normal cellularity A.S.C.-U.S. A.S.C.-H. L.S.I.L. H.S.I.L. Ca in situ Approx invasive A.C.G. A.I.S. adenocarcinoma Tasks and limitations of Citoscreener


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Cytofluorimetry: Principles and applications in pathological anatomy (3 hrs). Methods for the generation of 'conventional' murine models for the study of human pathology: transgenic mice, knock out mice, manteinance of colonies, 3R principle (7 hrs)



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· Knowledge of the pathology of the main and sub cellular structures
of the most important structural changes in the interstitial matrix· Knowledge of the main fields of application of diagnostic scanning electron microscopy and transmission·
Learning ultrastructural methods most commonly used in the study of
biological specimens for diagnostic purposes: basic protocol for the
preparation of samples for ultrastructural (TEM) for diagnostic
purposes; observation techniques; collection of documentation· Knowledge of the techniques used for electron microscopy as
indicated by the second choice in the diagnosis histopathology:
Inclusion methods for electron microscopy (TEM) samples already prepared
for histology or cytology·
Knowledge of the application of the diagnostic ultrastructural methods
of "special" (sections and pre-inclusion) necessary or useful to the
diagnosis; Histochemistry enzyme; immunohistochemistry.· Application of electron microscopy with reference to methods of cessation pro for Freeze-Fracture and Fracture-Label.· Determination of morphometric evaluation techniques and incubation in preparations adapted for the diagnostic ultrastructural


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ROBBINS E COTRAN - LE BASI PATOLOGICHE DELLE MALATTIE
Elsevier 2010


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teacher's lecture notes


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teacher's lecture notes and slides


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teacher's lecture notes


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The lessons are carried out with a teaching method in front.In case of emergency, distance lessons will be performed by using dedicated platforms (Moodle, Google Meet)




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Mandatory, in presence


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Oral examination on topics related to the program


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Oral examination on topics related to the program


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The final exam consists of an oral test that must be passed with the ≥ 18/30 score. The student have to demonstrate the acquisition of the knowledge of the proposed topics and also the ability to critical evaluate them.


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Oral examination on topics related to the program


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Features and aims of transgenic murine models
Features and aims of knock-out murine models
The 3R's principle
Basic principles of cytometry



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