Scientific Biomedical Communication

Activity type

Attività formative affini o integrative

SSD

BIO/11

Year

N/D

Semester

N/D

CFU

3

Hours distribution

24 classroom hours

Lecturers

ALBERTO BOFFI
ALBERTO BOFFI

Activity type

Attività formative affini o integrative

SSD

BIO/10

Year

N/D

Semester

N/D

CFU

6

Hours distribution

48 classroom hours

Lecturers

ALESSANDRA BONAMORE
ALESSANDRA BONAMORE

Module: Management and innovation in the biothecnologies I
The objective of the course is to make the student aware of the basic principles of the functioning of biomacromolecules and of the general metabolism.



Module: Management and innovation in the biothecnologies II
N/D

Module: Management and innovation in the biothecnologies I
Knowledge of basic principles of chemistry and biology


Module: Management and innovation in the biothecnologies II
N/D

Module: Management and innovation in the biothecnologies I
1. COMPOSITION OF THE LIVING MATERIAL
Notes on nucleotides, amino acids, proteins, carbohydrates and lipids. The main chemical reactions of biological interest: the bimolecular nucleophilic substitution, the redox reactions. Primary, secondary, tertiary and quaternary structure of proteins. Structure of nucleic acids. Visualization of the three-dimensional structure of proteins, the Protein Data Bank. Biological membranes.
2. SYSTEM OF PROTEINS AND ENZYMES
       Classification of enzymes and principles of enzymatic kinetics. The model of Michaelis and Menten. Transport proteins: albumin, hemoglobin and myoglobin. Enzymatic cofactors: NAD, FAD, heme group. Ionic channels and ionic pumps: potassium channels. Immunoglobulins and antigen-antibody recognition.
3. METABOLIC PROCESSES
Anabolism and catabolism, the processes of conversion of energy and matter into the biosphere. Glycolysis anaerobia. Cellular respiration and functioning of the mitochondrial respiratory chain. Photosynthesis, light phase and Calvin cycle. Fixation of nitrogen in the biosphere, role of nitrogenase.
4. CELLULAR INTEGRATED PROCESSES
Transcription and translation, the mechanism of protein synthesis. Contraction excitation coupling, calcium cycle and muscle contraction. The controlled protein degradation system: the proteasome and the ubiquitin ligases. Signal transduction.
5. PHYSICAL METHODOLOGIES IN THE ANALYSIS OF BIOLOGICAL SYSTEMS
Qualitative and quantitative interpretation of the interaction between light radiation and biological matter. The model of the optical electron, the model of Franck and Condon and its applications in UV-Vis-IR, fluorescence and Raman absorption spectroscopy. X-ray crystallography in determining the structure of proteins. Rapid kinetic methods for the determination of enzymatic activity.


Module: Management and innovation in the biothecnologies II
N/D

Module: Management and innovation in the biothecnologies I
Teaching material is provided in the form of resumee and slides
REFERENCES and BiBliography
1. Lehninger “Principles of Biochemistry” da consultazione sugli argomenti indicati nel programma.
2. Videos (Titoli)
“The Structure of DNA” (MIT x Bio)
“DNA translation and transcription” (Eslam Fahmy)
“Photosynthesis Light reaction, Calvin cycle, Electron Transport 3D Animation” (Encyclopédie Médicale)
“Photosynthesis (Light Reactions)” (ndsuvirtualcell)
“Gradients (ATP Synthases)” (ndsuvirtualcell)
http://www.sci.sdsu.edu/movies/actin_myosin_gif.html
3. PDB Molecules of the months (http://pdb101.rcsb.org/motm)
“ATP synthase, Hemoglobin, trypsin, cytochrome c oxidase, rubisco, potassium channels”
4. Research articles (reviews, open access)
- Caffarri S, Tibiletti T, Jennings RC, Santabarbara S. A comparison between plant photosystem I and photosystem II architecture and functioning. Curr Protein Pept Sci. 2014;15(4):296-331.
- Hu Y, Ribbe MW. A journey into the active center of nitrogenase. J Biol Inorg Chem. 2014 Aug;19(6):731-6.
5. Dispense su metodologie biofisiche




Module: Management and innovation in the biothecnologies II
N/D

Module: Management and innovation in the biothecnologies I
Teaching material is provided in the form of resumee and slides
REFERENCES and BiBliography
1. Lehninger “Principles of Biochemistry” da consultazione sugli argomenti indicati nel programma.
2. Videos (Titoli)
“The Structure of DNA” (MIT x Bio)
“DNA translation and transcription” (Eslam Fahmy)
“Photosynthesis Light reaction, Calvin cycle, Electron Transport 3D Animation” (Encyclopédie Médicale)
“Photosynthesis (Light Reactions)” (ndsuvirtualcell)
“Gradients (ATP Synthases)” (ndsuvirtualcell)
http://www.sci.sdsu.edu/movies/actin_myosin_gif.html
3. PDB Molecules of the months (http://pdb101.rcsb.org/motm)
“ATP synthase, Hemoglobin, trypsin, cytochrome c oxidase, rubisco, potassium channels”
4. Research articles (reviews, open access)
- Caffarri S, Tibiletti T, Jennings RC, Santabarbara S. A comparison between plant photosystem I and photosystem II architecture and functioning. Curr Protein Pept Sci. 2014;15(4):296-331.
- Hu Y, Ribbe MW. A journey into the active center of nitrogenase. J Biol Inorg Chem. 2014 Aug;19(6):731-6.
5. Dispense su metodologie biofisiche


Module: Management and innovation in the biothecnologies II
N/D

Module: Management and innovation in the biothecnologies I
The course is based on traditional lectures in class, with audio or video material availble on classroom.


Module: Management and innovation in the biothecnologies II
N/D

Module: Management and innovation in the biothecnologies I
Attendance to the lectures is not mandatory but strongly recommended.


Module: Management and innovation in the biothecnologies II
N/D

Module: Management and innovation in the biothecnologies I
In-depth manuscript on a topic of the program chosen by the student and oral exam.


Module: Management and innovation in the biothecnologies II
N/D

Module: Management and innovation in the biothecnologies I
planning of a scientific publication.
Enzime kinetics. Photosyntesys. In vitro evolution.


Module: Management and innovation in the biothecnologies II
N/D

Module: Management and innovation in the biothecnologies I



Module: Management and innovation in the biothecnologies II
N/D