Biomedical Laboratory techniques - Course C - ASL Latina (distretto nord)

Activity type

Scienze e tecniche di laboratorio biomedico

SSD

BIO/12

Year

2nd year

Semester

2nd semester

CFU

2

Hours distribution

20 classroom hours

Lecturers

MICHELE ZAMPIERI

Activity type

Scienze e tecniche di laboratorio biomedico

SSD

MED/46

Year

2nd year

Semester

2nd semester

CFU

2

Hours distribution

20 classroom hours

Lecturers

ELENA DE FALCO

Activity type

Scienze e tecniche di laboratorio biomedico

SSD

MED/05

Year

2nd year

Semester

2nd semester

CFU

2

Hours distribution

20 classroom hours

Lecturers

ESTERINA PASCALE

The course aims to provide students with the knowledge necessary to develop the skills required to understand the principles underlying the main techniques and protocols developed for diagnostic laboratory medicine. In addition, students will acquire knowledge relating to the role, purposes and new trends in the development of laboratory medicine, the operational logic and general organisation of laboratories, and the characteristics of biochemical, haematological and bacteriological analysers. Students will acquire the knowledge and skills necessary to understand laboratory tests for the study of renal, hepatic and haematopoietic system function, clinical enzymology, carbohydrate and lipid metabolism, immunohematological diseases, haemostatic and haemocoagulative disorders, as well as specific issues in transfusion medicine, with a view to applying this knowledge in diagnostic techniques.

In order to understand the course content and achieve the learning objectives, knowledge of human anatomy, pathophysiology and biochemistry is essential.

Module: CLINICAL BIOCHEMISTRY
Haemochromocytometric examination: generalities. Morpho-functional study of erythrocyte pathologies.
Thalassemias and haemoglobinopathies. Erythrocyte enzymes. Iron deficiency and overload. Anaemias.
Morpho-functional study of leukocyte pathologies.
Laboratory diagnostics of haemocoagulation and thrombosis. Seroproteins. Lipoproteins.


Module: LABORATORY METHODS
Esame delle urine.
Elettroliti.
Emogasanalisi
Waaler Rose
Pneumococco pneumoniae
Detection di Rota, Adeno, Astro e Norovirus
Legionellosi
Test diagnostico rapido in vitro per la determinazione di E.coli O157 in campione di feci -



Module: SYSTEMATIC CLINICAL PATHOLOGY

Laboratory methods useful for molecular diagnostics: Southern blot, PCR and DNA sequencing. Cytogenetic methods.
Genetic markers of neoplasia.
Clinical evaluation of the heart muscle. Laboratory diagnosis of myocardial infarction, early and late indicators.
Clinical evaluation of liver function and the hepatobiliary system. Markers of alcoholism.
Laboratory evaluation of pancreatic function: exocrine and endocrine. Glucose determination, glucose tolerance test. Type I and II diabetes.
Markers of inflammation and sepsis.
Mass spectrometry and Tandem mass.

Module: CLINICAL BIOCHEMISTRY
Lecture notes
Ciaccio, M., Lippi, G. Biochimica clinica e medicina di laboratorio. EDISES


Module: LABORATORY METHODS
N/D
Module: SYSTEMATIC CLINICAL PATHOLOGY
M. Ciaccio-Elementi di Biochimica clinica e Medicina di laboratorio. Edises
G. Federici-Medicina di laboratorio McGraw-Hill

Module: CLINICAL BIOCHEMISTRY
N/D
Module: LABORATORY METHODS
N/D
Module: SYSTEMATIC CLINICAL PATHOLOGY
N/D

The adopted teaching model primarily consists of lectures, utilizing PowerPoint slides as telematic support. To enhance the student active engagement, it will be scheduled also didactic moments inspired by the "flipped classroom" approach. The lesson slides will be posted on the course Google Classroom platform before each lecture, allowing students to review them in advance.

To take the exam related to the integrated course, it is necessary to have documented the presence in the classroom of at least 67% of the hours corresponding to the credits assigned to the integrated course. The attendance will be measured by roll call at the beginning of the lesson.

The exam consists of an interview to evaluate the student's knowledge of the topics covered during the course, clarity of exposition, and property of language. To pass the exam, achieving a grade of not less than 18/30 in each module is necessary by demonstrating sufficient knowledge of the course content and expounding the topics enough. To achieve a score of 30/30 cum laude, the student must demonstrate excellent knowledge of all the topics covered during the course, being able to link them logically and with appropriate scientific language.

PCR and primer design.
Cytogenetic methods.
Genetic markers of neoplasia. Liquid biopsy.
Clinical evaluation of the heart muscle. Laboratory diagnosis of myocardial infarction, early and late indicators.
Clinical evaluation of liver function and the hepatobiliary system
Markers of alcoholism
Glucose determination, glucose tolerance test.
Markers of inflammation
Mass spectrometry and Tandem mass.
What hemoglobin values define anemia?
What does an increase in white blood cells indicate?
What is the clinical significance of thrombocytopenia?
What are the main proteins present in serum?
What is the purpose of serum protein electrophoresis?
What does an increase in C-reactive protein indicate?
What are the main parameters of the lipid profile?
What is the difference between LDL cholesterol and HDL cholesterol?
Which lipoprotein is considered atherogenic?
What does having elevated triglycerides mean?

Module: CLINICAL BIOCHEMISTRY



Module: LABORATORY METHODS
N/D
Module: SYSTEMATIC CLINICAL PATHOLOGY
N/D