Biomedical Laboratory techniques - Course A - Roma Azienda Policlinico Umberto I

Activity type

Scienze e tecniche di laboratorio biomedico

SSD

MED/08

Year

2nd year

Semester

1st semester

CFU

2

Hours distribution

20 classroom hours

Lecturers

RITA ALAGGIO

Activity type

Scienze e tecniche di laboratorio biomedico

SSD

MED/08

Year

2nd year

Semester

1st semester

CFU

1

Hours distribution

10 classroom hours

Lecturers

ANNA CRESCENZI

Activity type

Scienze e tecniche di laboratorio biomedico

SSD

MED/46

Year

2nd year

Semester

1st semester

CFU

1

Hours distribution

10 classroom hours

Lecturers

ANNALINDA PISANO

Activity type

Scienze e tecniche di laboratorio biomedico

SSD

MED/08

Year

2nd year

Semester

1st semester

CFU

1

Hours distribution

10 classroom hours

Lecturers

ANNALINDA PISANO

Activity type

Scienze e tecniche di laboratorio biomedico

SSD

MED/46

Year

2nd year

Semester

1st semester

CFU

1

Hours distribution

10 classroom hours

Lecturers

ANNALINDA PISANO

The integrated course aims to provide students with a solid theoretical and methodological foundation in macroscopic and microscopic observation techniques applied to pathological anatomy, as well as in the fundamental principles of histological, cytological, and electron microscopy methods. Upon completion of the course, students will be able to appropriately apply macro- and microscopic observation techniques and actively contribute to the anatomo-pathological diagnostic process, integrating technical expertise with clinical requirements. Students will also develop the ability to interpret and critically evaluate the quality of histological and cytological preparations, identifying potential technical errors or artifacts in relation to the pathological and clinical context.

Students are expected to have basic knowledge and theoretical–practical foundations acquired through prior study of laboratory methodologies.

Module: Pathological Anatomy Institutions
Study of the mechanisms of disease, with particular emphasis on cell injury and the morphological alterations associated with inflammatory processes and neoplastic diseases.
The Pathology Laboratory in everyday practice: a survival guide for Biomedical Laboratory Technicians. Risks, critical issues, and unexpected challenges: how to recognize and prevent them.
Understanding clinicopathological terminology and its significance in the management of biological specimens: from specimen preservation for specific diagnostic investigations to the assessment of surgical resection margins.
The role of the Biomedical Laboratory Technician in precision medicine: from specimen management for molecular testing to immunohistochemical characterization.

Module: Laboratory Medicine Techniques:
Pathological Anatomy: Historical Background.
Purpose of the Anatomo-pathological Diagnosis.
Diagnostic Process in Pathological Anatomy.
Pathology 2.0: Extraction Techniques for Diagnostic Purposes.
The Role of the Laboratory Technician and New Specialized Roles.
Digital Pathology.

Module: Techniques in Anatomical Pathology:
Principles of immunohistochemistry and immunofluorescence: immunological basis and detection systems.
Pre-analytical and analytical phases and quality control: positive and negative controls, protocol optimization, and recognition and management of technical artifacts.
Interpretation of results and diagnostic applications: staining patterns and their correlation with morphological findings.

Module: Ultrastructural Techniques:
Principles of Microscopy: General introduction to Electron Microscopy, historical background, and comparison with optical microscopy.
Transmission Electron Microscopy (TEM): Preparation and evaluation of biological samples (collection, fixation, embedding, sectioning, staining).
Scanning Electron Microscopy (SEM): Preparation and evaluation of biological samples.
Description of different biological specimens (cells, tissues).
Image Analysis.
Biomedical Applications of Electron Microscopy.

Module: Cytopathology:
Types of samples: exfoliative cytology, sediment cytology, fine needle aspiration cytology,
brushing cytology, echoendoscopy samples.
Preparation of cytological samples: cytological smear, thin layer cytology, cytospin,
cytocentrifugation, cell block.
Cytodiagnostic techniques: basic staining, special staining, immunohistochemical staining.
Principles of cell morphology assessment: benign, inflammatory and malignant cytological
characteristics.
Molecular analysis techniques.
Digital cytology.
Diagnostic report requirements and organ-specific reporting categories.

Testo: Ruco-Scarpa. Anatomia Patologica, Le basi, Ed. Utet -Scienze Mediche

Module: INSTITUTIONS OF PATHOLOGICAL ANATOMY
N/D
Module: CYTOPATHOLOGY
N/D
Module: TECHNOLOGIES OF LABORATORY MEDICINE
N/D
Module: TECHNOLOGIES OF PATHOLOGICAL ANATOMY
N/D
Module: ULTRASTRUCTURAL TECHNIQUES
N/D

The course is conducted through lectures

Attendance is mandatory and in person.
In order to take the final assessment, students must have achieved the attendance percentage required by the Degree Program.

The final grade will be awarded based on the results of the assessments taken for learning evaluation, which consists of a oral examination.
Students who have achieved the required attendance percentage are admitted to take the exam tests.

1. When is the cytocentrifuge used in a body fluid sample?

Module: INSTITUTIONS OF PATHOLOGICAL ANATOMY
N/D
Module: CYTOPATHOLOGY
N/D
Module: TECHNOLOGIES OF LABORATORY MEDICINE
N/D
Module: TECHNOLOGIES OF PATHOLOGICAL ANATOMY
N/D
Module: ULTRASTRUCTURAL TECHNIQUES
N/D