Health Professions of Technical Sciences (diagnostic) - Course A - Roma Azienda Policlinico Umberto I

Activity type

* Scienze e tecniche di laboratorio biomedico

SSD

MED/08

Year

2nd year

Semester

1st semester

CFU

2

Hours distribution

20 classroom hours

Lecturers

FRANCESCA ROMANA BUTTARELLI

Activity type

* Scienze e tecniche di neurofisiopatologia

SSD

MED/50

Year

2nd year

Semester

1st semester

CFU

2

Hours distribution

20 classroom hours

Lecturers

LUCIA DI MARCOTULLIO
MARIA HELENA STABILE

Activity type

* Scienze e tecniche di laboratorio biomedico

SSD

MED/07

Year

2nd year

Semester

1st semester

CFU

2

Hours distribution

20 classroom hours

Lecturers

MARCO ARTINI

Activity type

* Scienze e tecniche di laboratorio biomedico

SSD

MED/04

Year

2nd year

Semester

1st semester

CFU

2

Hours distribution

20 classroom hours

Lecturers

RICCIARDA GALANDRINI

Knowledge and ability to understand the cellular and molecular mechanisms underlying the development of diseases; knowledge of the main genetic, cytogenetic and molecular techniques for the diagnosis of diseases.

Knowledge of the basic fundamentals of preparatory subjects in the health area, such as biology, chemistry, physics, mathematics, in particular, knowledges of basic cellular and molecular biology

Module: PATHOLOGICAL ANATOMY
N/D
Module: APPLIED MEDICAL TECHNICAL SCIENCES
- General pathology: etiology and pathogenesis; aspects of morbid phenomena; cell damage (regressive – expansive; reversible – irreversible); carcinogenesis.
- Genetic pathology: karyotype; molecular cytogenetic techniques (banding techniques of chromosome, FISH, SKY); DNA alterations: genomic, chromosomal and gene mutations; examples of diseases with genetic transmission: Fragile X syndrome, Duchenne dystrophy, Cystic fibrosis, Sickle cell anemia
- Molecular pathology: diagnostic molecular biology techniques (restriction enzymes, Southern blot, PCR, direct DNA sequencing, deep sequencing).



Module: MICROBIOLOGY AND CLINICAL MICROBIOLOGY
Hints on bacterial cell, pathogens and saprophites, virulence factors, biofilm; viruses (structure and replication), fungi.
Bacterial identification.
Protection of the personnel and good laboratory practice: classification of microrganisms according to the personnel risk (levels 1 -> 4)
General safety practices in the microbiology lab
Management of the biological samples in microbiology
Disinfection and sterilization
Antibiotics and disinfectants, antibioticresistance and antibiogram
Materials and techniques in microbiology
Workflow and equipments



Module: GENERAL PATHOLOGY
Monoclonal antibodies and their applications in laboratory medicine, imaging, and therapy. Autoimmune diseases, allergic diseases: pathogenic and diagnostic aspects.

Module: PATHOLOGICAL ANATOMY
The teaching materials for each lesson will be made available to students through Sapienza’s online learning platform.


Module: APPLIED MEDICAL TECHNICAL SCIENCES
- Elementi di Patologia Generale, Pontieri, Piccin
- Biologia cellulare e genetica, Parte Seconda – GENETICA, Fantoni, Tripodi, Piccin
- Slides


Module: MICROBIOLOGY AND CLINICAL MICROBIOLOGY
EUDES LANCIOTTI:
MICROBIOLOGIA CLINICA Last edition
Editor AMBROSIANA

Mary Louise Turgeon
MEDICINA DI LABORATORIO Last edition
EDRA SpA


Module: GENERAL PATHOLOGY
- IMMUNOLOGIA - EdiSES
Richard Coico, Geoffrey Sunshine, Eli Benjamini
-LE BASI DELL’IMMUNOLOGIA-Zanichelli
Thao Doan, Roger Melvold, Susan Viselli, Carl Waltenbaugh

Module: PATHOLOGICAL ANATOMY
N/D
Module: APPLIED MEDICAL TECHNICAL SCIENCES
N/D
Module: MICROBIOLOGY AND CLINICAL MICROBIOLOGY
N/D
Module: GENERAL PATHOLOGY
N/D

Classroom teaching

Mandatory

Written and oral presentation

Alterations of chromosome numbers and structure

Module: PATHOLOGICAL ANATOMY



Module: APPLIED MEDICAL TECHNICAL SCIENCES

• Introduction to general pathology: etiology and pathogenesis, aspects of disease processes, cellular damage, neoplasms (2 lessons)
  • Books: Recommended
    
    texts plus teaching materials provided by the instructor 


• DNA alterations: genomic, chromosomal, and gene mutations; examples of genetic diseases; karyotype and classical and molecular cytogenetic techniques (banding techniques, FISH, SKY) (3 lessons)
  • Books: Recommended
    
    texts plus teaching materials provided by the instructor 


• Molecular biology techniques for diagnostic purposes (2 lessons)
  • Books: Recommended
    
    texts plus teaching materials provided by the instructor 

• Natural immune responses
  • Books: Recomended text, teaching matherial provided on Moodle 


• Adaptive immune rsponse: antibodies structure and functions
  • Books: Recomended text, teaching matherial provided on Moodle 


• Monoclonal antibodies: development, engineering, dignostic and therapeutic applications
  • Books: Recomended text, teaching matherial provided on Moodle 


• Allergic diseases
  • Books: Recomended text, teaching matherial provided on Moodle 


• Self-assessment section