Medicine and Surgery

Activity type

Medicina di laboratorio e diagnostica integrata

SSD

MED/05

Year

3rd year

Semester

1st semester

CFU

2

Hours distribution

24 classroom hours

Lecturers

ESTERINA PASCALE

Activity type

Medicina di laboratorio e diagnostica integrata, Tirocini formativi e di orientamento

SSD

BIO/12, BIO/12

Year

3rd year

Semester

1st semester

CFU

2

Hours distribution

12 classroom hours, 25 others hours

Lecturers

GIAMPIERO FERRAGUTI

Activity type

Medicina di laboratorio e diagnostica integrata, Tirocini formativi e di orientamento

SSD

MED/07, MED/07

Year

3rd year

Semester

2nd semester

CFU

2

Hours distribution

12 classroom hours, 25 others hours

Lecturers

DANIELA SCRIBANO

Activity type

Medicina di laboratorio e diagnostica integrata, Tirocini formativi e di orientamento

SSD

BIO/12, BIO/12

Year

3rd year

Semester

2nd semester

CFU

4

Hours distribution

24 classroom hours, 50 others hours

Lecturers

ANDREA FUSO
GIAMPIERO FERRAGUTI

Activity type

Medicina di laboratorio e diagnostica integrata

SSD

MED/05

Year

3rd year

Semester

2nd semester

CFU

1

Hours distribution

12 classroom hours

Lecturers

ESTERINA PASCALE

Essential Objectives of the Integrated Course
Upon completion of the course, the student must:
- know the biochemical-molecular parameters, in biological samples and in vivo, at the different levels of structural and functional organization: from molecules to cells, tissues, organs, and the whole organism.
- to know how to critically interpret laboratory findings also in relation to pathophysiological states.
- to know how to use laboratory findings for the purposes of diagnosis and prognosis.

Essential Objectives of the Integrated Course
Upon completion of the course, the student must:
- know the biochemical-molecular parameters, in biological samples and in vivo, at the different levels of structural and functional organization: from molecules to cells, tissues, organs, and the whole organism.
- to know how to critically interpret laboratory findings also in relation to pathophysiological states.
- to know how to use laboratory findings for the purposes of diagnosis and prognosis.

Course materials are available for download on the e-learning platform at: https://elearning.uniroma1.it/course/view.php?id=316

Knoledge of the concepts from the courses of Chenistry, Biochemistry, Pathology, Microbiology

Main lipoprotein classes, enzymatic activities, receptors and apoproteins involved in lipid metabolism. Intracellular and systemic regulation of cholesterol. Classification of dyslipidemias. Reference values for cholesterol and triglycerides, concept of cardiovascular risk, atherosclerotic disease. Laboratory diagnostics of dyslipidemia.

Main cardiovascular risk factors. Laboratory diagnostics of myocardial infarction: creatine kinase, creatine kinase MB fraction, myoglobin, troponins, aspartate aminotransferase, lactic dehydrogenase.

Iron, protoporphyrin and globins: pathophysiology, clinical biochemistry and laboratory diagnostics. Iron deficiency anemias, porphyrias, thalassemias and hemoglobinopathies.

Automated blood count: operating principles, examples of equipment, main parameters relating to erythrocytes, leukocytes and platelets. Examples of changes in the blood count. Classification of anemia and relationship with the parameters of the blood count.

Pathophysiology, Clinical Biochemistry and laboratory diagnostics of hemostasis. Review of the vascular and platelet phases; the coagulation system and the fibrinolytic system: main plasma factors involved and their alterations. Laboratory diagnostics in the exploration of the platelet phase and the coagulation and fibrinolytic system, also in relation to thrombotic and hemorrhagic states.

Antonozzi I., Gulletta E.: Medicina di Laboratorio Logica e Patologia clinica. Editore Piccin Nuova Libreria S.p.A.
Ciaccio M., Lippi G.: Biochimica clinica e Medicina di Laboratorio. EdiSES II edizione
Roberto Cevenini: Microbiologia Clinica (seconda edizione )- Piccin
Bizzarri M.: Interpretazione clinica degli esami di laboratorio. Piccin.

Module: Laboratory Medicine I - Clinical Pathology
N/D
Module: Laboratory Medicine I - Clinical Biochemistry and Clinical Molecular Biology
N/D
Module: Laboratory Medicine I - Clinical Pathology
N/D
Module: Laboratory Medicine II - Clinical Biochemistry and Clinical Molecular Biology
N/D
Module: Laboratory Medicine II - Microbiology and Clinical Microbiology
N/D

Front lessons

In presence

Oral exam composed of two stages: clinical biochemistry and clinical patholgy with one f the to professors, clinical microbiology with the related professor

Description of lab analyses, in relation to the physio-pathogenic condition and with details of technical aspects and reference values

Module: Laboratory Medicine I - Clinical Pathology

• Strategy in requesting laboratory tests: screening tests, individual tests, organ profiles, diagnostic protocols, therapy monitoring, diagnostic investigations.4h
  • Books: Ciaccio M. Trattato di Biochimica Clinica e Medicina di Laboratorio. EdiSES


• Function and lesion markers, their pathophysiological significance, time of appearance and half-life in biological fluids: early and late markers. Clinical application: specific and non-specific markers. 4h
  • Books: Ciaccio M. Trattato di Biochimica Clinica e Medicina di Laboratorio. EdiSES


• Phenotypic tumour markers: requirements for the ideal marker and clinical application. Concept of threshold (standard/pathological/neoplastic). Oncofetal and tumour-associated antigens, tumour-secreted hormones, enzymes and proteins as tumour markers. 4h
  • Books: Ciaccio M. Trattato di Biochimica Clinica e Medicina di Laboratorio. EdiSES


• Genotypic tumour markers: importance and limitations of their characterisation, relevance of the investigation methodology. The concept of minimal residual disease. Immunometric techniques.4h
  • Books: Ciaccio M. Trattato di Biochimica Clinica e Medicina di Laboratorio. EdiSES


• Enzymes as markers of function and injury. Diagnostic significance of plasma enzymes: recognition of the type, extent and severity of the injury, organ recognition. Isoenzymes. Isoforms. Enzyme profiles and organ enzymograms. 4h
  • Books: Ciaccio M. Trattato di Biochimica Clinica e Medicina di Laboratorio. EdiSES


• Mass spectrometry: principles and main applications. 4h
  • Books: Ciaccio M. Trattato di Biochimica Clinica e Medicina di Laboratorio. EdiSES

• Appropriateness
  in Laboratory Medicine.
  • Books: Ciaccio. Trattatato di Biochimica Clinica e Medicina di Laboratorio. Edizioni Edises.


• Main
  analytical methods.
  • Books: Ciaccio. Trattatato di Biochimica Clinica e Medicina di Laboratorio. Edizioni Edises.


• Variability
  in laboratory medicine and quality controls.
  • Books: Ciaccio. Trattatato di Biochimica Clinica e Medicina di Laboratorio. Edizioni Edises.


• How
  to interpret a laboratory survey.
  • Books: Ciaccio. Trattatato di Biochimica Clinica e Medicina di Laboratorio. Edizioni Edises.


• Reference
  intervals, critical values and decision limits
  • Books: Ciaccio. Trattatato di Biochimica Clinica e Medicina di Laboratorio. Edizioni Edises.

• General
  Principles of Clinical Microbiology and Methods in Clinical Microbiology


• Microbial identification methods, antibiogram, antibiotic-resistance


• Nervous and circulatory system infections


• Respiratory infections


• Gastrointestinal infections


• Urinary and genital infections

• Blood analysys


• Anemias


• Anemias and iron


• Thalassemias and haemoglobinopatias


• Macrocytic anemia-molecular analyses


• Haenostasis


• Myocardial infarcion


• Lipoproteins and lipids


• Epiphisis-hypophisis-tyroid


• paratyroids-surrenal glands


• Testis- Ovarium

• Laboratory tests for the pathophysiological assessment of the main functional and structural alterations affecting the liver. Bilirubin metabolism disorders. Proteins, liver enzymes. Laboratory tests in the assessment of patients with chronic passive congestion and biliary tract obstruction. Laboratory tests for the assessment of acute and chronic hepatitis. Alterations in laboratory parameters in patients with cirrhosis and fulminant liver damage. The clinical and diagnostic significance of autoantibody determination in autoimmune diseases of the liver and biliary tract. Laboratory diagnosis of liver neoplasms. 4h
  • Books: Ciaccio M. Trattato di Biochimica Clinica e Medicina di Laboratorio. EdiSES


• Laboratory tests for assessing endocrine pancreatic function: insulin, glucagon, incretin, somatostatin. Glucose determination. Type I diabetes mellitus. Type II diabetes mellitus. Hypoglycaemia. 4 hours
  • Books: Ciaccio M. Trattato di Biochimica Clinica e Medicina di Laboratorio. EdiSES


• Biochemical indicators of bone metabolism: calcium, phosphorus, magnesium; hormones regulating mineral metabolism. Mineral metabolism disorders: hypercalcaemia, hypocalcaemia, hyperphosphataemia, hypophosphataemia, hypermagnesaemia, hypomagnesaemia. Biochemical indicators of bone remodelling: markers of bone resorption and formation. Metabolic bone diseases: osteoporosis, osteomalacia, Paget's disease.4h
  • Books: Ciaccio M. Trattato di Biochimica Clinica e Medicina di Laboratorio. EdiSES