Biochemistry

Activity type

Discipline biotecnologiche comuni

SSD

BIO/10

Year

1st year

Semester

2nd semester

CFU

3

Hours distribution

24 classroom hours

Lecturers

MARIA CARMELA BONACCORSI DI PATTI

Activity type

Discipline farmaceutiche

SSD

CHIM/11

Year

1st year

Semester

2nd semester

CFU

3

Hours distribution

24 classroom hours

Lecturers

CRISTINA MAZZONI

The course of "Biochemical Biotechnologies I" consists of the integrated modules "Applied Biochemistry" and "Microbial Biotechnologies: Industrial Applications". The learning outcomes of the course concern biotechnological applications of enzymes, proteins and microorganisms in basic and applied research and in industry. The learning outcomes of each individual module can be consulted in the appropriate section of the module.

The course of "Biochemical Biotechnologies " consists of the integrated modules "Applied Biochemistry" and "Microbial Biotechnologies: Industrial Applications". The learning outcomes of the course concern biotechnological applications of enzymes, proteins and microorganisms in basic and applied research and in industry. The learning outcomes of each individual module can be consulted in the appropriate section of the module.

The course of Biochemical Biotechnologies in the master course is in the first year. Basic knowledge of Cell Biology, Microbiology, Genetics, Biochemistry and Molecular Biology is required.

Module: MICROBIAL BIOTECHNOLOGIES: INDUSTRIAL APPLICATIONS
The course includes 20 hours of classroom lectures and 4 hours of guided exercises.
Biotechnological applications of enzymes. Production of recombinant enzymes and proteins: methods and strategies. Protein engineering: site-specific mutagenesis,
in vitro evolution. Production of therapeutic proteins (hormones, antibodies, coagulation factors). Main techniques of cell and enzyme immobilization. Biocatalysts: use of enzymes for production of biotechnologically relevant compounds.
Multienzymatic systems for biosynthesis of bioactive secondary metabolites. Non-ribosomal biosynthesis of peptides. Enzyme mechanism of peptide synthetases. Examples (gramicidin, surfactin, penicillins and cefalosporins). Biosynthesis of polyketides. Mechanism of polyketide formation and analogies with fatty acid biosynthesis. Examples (erythromycin, statins).



Module: APPLIED BIOCHEMISTRY
The course includes 20 hours of frontal teaching and 4 hours of guided exercises.

Definition of microbial fermentations
The key elements of fermentation development (strain selection and optimisation, media, process development, scale-up).
Physiology, biochemistry and genetics of the main microorganisms used in industrial microbial biotechnology
Principles of synthetic biology and metabolic engineering
Biosensors
Understanding of the main genome editing techniques on microorganisms of industrial interest
Examples of optimized microrganisms for production of industrial relevant products.

Module: MICROBIAL BIOTECHNOLOGIES: INDUSTRIAL APPLICATIONS
There is no specific text book. Teaching materials (slides of the lessons and scientific papers) are published on the educational site Elearning2 at http://elearning2.uniroma1.it


Module: APPLIED BIOCHEMISTRY
- Biotecnologie microbiche S. Donadio e G. Marino. Casa EditriceAmbrosiana.(2008)
- Chimica e biotecnologia delle fermentazioni industriali. MM Bianchi. Edizioni Nuova Cultura. (2009)
- Microbiologia Industriale, Matilde Manzoni. Casa Editrice Ambrosiana. ISBN 88-408-1320-9
- Lesson slides (available on the E-learning platform)
- Scientific papers

Module: MICROBIAL BIOTECHNOLOGIES: INDUSTRIAL APPLICATIONS
N/D
Module: APPLIED BIOCHEMISTRY
It will be provided by the professor during the lessons

classroom lectures and small group activities

attendance is not mandatory but strongly advised

oral presentation of a paper from the scientific literature on topics of the course program

The questions will focus on the specific scientific work presented. Much emphasis will be given to the following criteria:
Description of the background and rationale of the work
Description of the experimental setup
Techniques used and for what purpose
Discussion of the results and possible perspectives

Module: MICROBIAL BIOTECHNOLOGIES: INDUSTRIAL APPLICATIONS
N/D
Module: APPLIED BIOCHEMISTRY
N/D