Medical Biotechnology

Activity type

Discipline biotecnologiche comuni

SSD

BIO/10

Year

N/D

Semester

N/D

CFU

4

Hours distribution

32 classroom hours

Lecturers

GIORGIO GIARDINA
GIUSEPPINA MIGNOGNA

Activity type

Discipline biotecnologiche comuni

SSD

BIO/10

Year

N/D

Semester

N/D

CFU

2

Hours distribution

16 classroom hours

Lecturers

Activity type

Discipline biotecnologiche comuni

SSD

BIO/11

Year

N/D

Semester

N/D

CFU

3

Hours distribution

24 classroom hours

Lecturers

Understand the main methodologies for determining protein structures.
Learn the main methods used in proteomic analysis, the types of data generated, and their key limitations.
Determine the thermodynamic stability of proteins and understand their mechanisms of folding. Mechanisms of aggregation and fibrillogenesis. Intermolecular interactions: binding affinity and association/dissociation rate constants.
Learn the general principles of the design, heterologous production and mutagenesis of proteins.
Acquire capacity of critical reading of scientific papers.

Module: Structural biology and protein engineering I


At the end of the course, the student will have to demonstrate the following (the relevant Dublin descriptors are indicated in brackets):
-Knowledge of the main methodologies used in proteomics (Knowledge and understanding)
-The ability to learn and critically select the continuous information that the scientific community generates (Knowledge and ability to apply understanding)
-The ability to independently evaluate the results obtained in proteomics experiments and to indicate the main methodologies relevant to the analysis. (Autonomy of judgment)
-The acquisition of an appropriate language to discuss biochemical issues (Communication skills)
-The ability to update or expand one's knowledge by independently drawing on texts, scientific articles, and databases relating to cellular metabolism's biochemical/molecular mechanisms studied with proteomic approaches. (Learning ability)



Module: Structural biology and protein engineering II
N/D
Module: Structural biology and protein engineering III
N/D

Module: Structural biology and protein engineering I
knowledge of basic concepts of protein structure, including chemistry of peptide bonds, name and chemical structure of amino acids; structural organization of proteins (primary to quaternary); basic notions of waves and of electric and magnetic fields.


Module: Structural biology and protein engineering II
N/D
Module: Structural biology and protein engineering III
N/D

Module: Structural biology and protein engineering I
Proteomics: Principles of mass spectrometry. Ionization methods. Mass analyzers. LC-mass spectrometry.
Main tools of proteomic analysis and protein identification.
Experimental approach for systematic, differential and functional proteomics.
The course includes a lesson on the analysis of proteomic data obtained from mass spectrometry experiments



Module: Structural biology and protein engineering II
N/D
Module: Structural biology and protein engineering III
N/D

Module: Structural biology and protein engineering I
Selected literature reviews will be indicated during the course and on the e-learning platform.


Module: Structural biology and protein engineering II
N/D
Module: Structural biology and protein engineering III
N/D

Module: Structural biology and protein engineering I
N/D
Module: Structural biology and protein engineering II
N/D
Module: Structural biology and protein engineering III
N/D

Module: Structural biology and protein engineering I
Classroom lessons.
Laboratory lessons on selected topics.



Module: Structural biology and protein engineering II
N/D
Module: Structural biology and protein engineering III
N/D

Module: Structural biology and protein engineering I
Mandatory attendance


Module: Structural biology and protein engineering II
N/D
Module: Structural biology and protein engineering III
N/D

Module: Structural biology and protein engineering I
The student must show a basic knowledge of biochemistry and structural biology, together with the capacity to logically connect and apply this knowledge by solving simple experimental problems.


Module: Structural biology and protein engineering II
N/D
Module: Structural biology and protein engineering III
N/D

Module: Structural biology and protein engineering I
What are the experimental approaches for a differential proteomic experiment?
Describe the ionization methods used in mass spectrometry.
What is Peptide mass fingerprint?


Module: Structural biology and protein engineering II
N/D
Module: Structural biology and protein engineering III
N/D

Module: Structural biology and protein engineering I

• The topics of the lessons will follow the order of the lessons available online, but are subject to change at the discretion of the teacher.
  • Books: Lessons Slides in PDF format + other material provided during the course.


• Prof. G. Mignogna: lectures scheduled from October 23 to November 12
  • Books: Lessons Slides in PDF format + other material provided during the course.


• Prof. G. Giardina: lectures scheduled from November 14 to December 17
  • Books: Lessons Slides in PDF format + other material provided during the course.