Medicine and Surgery "B" - Roma Azienda Policlinico Umberto I

Activity type

B_02. Struttura, funzione e metabolismo delle molecole d'interesse biologico, C_19. Formazione clinica interdisciplinare e medicina basata sulle evidenze e medicina di precisione

SSD

BIOS-07/A, BIOS-07/A

Year

1st year

Semester

2nd semester

CFU

4

Hours distribution

50 classroom hours

Lecturers

ALBERTO MACONE
CARLOTTA ZAMPARELLI

Activity type

B_02. Struttura, funzione e metabolismo delle molecole d'interesse biologico

SSD

BIOS-08/A

Year

1st year

Semester

2nd semester

CFU

2

Hours distribution

25 classroom hours

Lecturers

CARLOTTA ZAMPARELLI

Activity type

B_02. Struttura, funzione e metabolismo delle molecole d'interesse biologico

SSD

BIOS-08/A

Year

N/D

Semester

N/D

CFU

3

Hours distribution

37.5 classroom hours

Lecturers

Activity type

B_02. Struttura, funzione e metabolismo delle molecole d'interesse biologico, C_19. Formazione clinica interdisciplinare e medicina basata sulle evidenze e medicina di precisione, Attività formative affini o integrative

SSD

BIOS-07/A, BIOS-07/A, BIOS-07/A

Year

N/D

Semester

N/D

CFU

7

Hours distribution

88 classroom hours

Lecturers

Activity type

B_01. Discipline generali per la formazione del medico

SSD

BIOS-10/A

Year

1st year

Semester

2nd semester

CFU

2

Hours distribution

25 classroom hours

Lecturers

CARLA CICCHINI

At the end of the Biochemistry course, the student will have acquired a broad knowledge of the main classes of biomolecules in terms of structure, function, and reactivity. The student will also have gained an understanding of the molecular basis of metabolism and will be able to explain metabolic pathways and their mechanisms of integration and regulation.

Module: BIOCHEMISTRY I - BIOCHEMISTRY
N/D
Module: BIOCHEMISTRY I - MOLECULAR BIOLOGY
N/D
Module: BIOCHEMISTRY I - APPLIED BIOLOGY
N/D
Module: BIOCHEMISTRY II - BIOCHEMISTRY
N/D
Module: BIOCHEMISTRY II - MOLECULAR BIOLOGY
N/D

Module: BIOCHEMISTRY I - BIOCHEMISTRY
N/D
Module: BIOCHEMISTRY I - MOLECULAR BIOLOGY
N/D
Module: BIOCHEMISTRY I - APPLIED BIOLOGY
N/D
Module: BIOCHEMISTRY II - BIOCHEMISTRY
N/D
Module: BIOCHEMISTRY II - MOLECULAR BIOLOGY
N/D

Module: BIOCHEMISTRY I - BIOCHEMISTRY
N/D
Module: BIOCHEMISTRY I - MOLECULAR BIOLOGY
GENERAL CHARACTERISTICS OF LIVING ORGANISMS
Water, organic compounds, minerals, trace elements, biological buffers.

ENZYMES
General concepts. Catalyst and enzyme catalysis. Enzyme specificity: the active site. Mechanism of enzyme action. Principles of enzyme kinetics: reaction rate, effect of temperature ad pH. Michaelis-Menten equation.
Enzyme inhibition: irreversible, competitive and noncompetitive
Allosteric enzymes. Regulation of enzyme activity. Isoenzymes. Enzyme classification. Coenzymes and cofactors: structure and function.

PROTIDES
Amino acids: classification, structure, optical activity, dissociation, isoelectric and isoionic points. Essential amino acids. Peptide bond. Peptides. Proteins: general properties, classification, methods of study, purification, general reactions, dissociable groups, isoelectric point. Primary, secondary, tertiary and quaternary structures. Protein denaturation. Mechanisms of regulation of protein function: allostery, covalent modifications, proteolytic cleavage. Proteins of particular biological interest: plasma proteins, immunoglobulins, collagen, membrane proteins, hemeproteins. Structure and function of myoglobin and hemoglobin: transport of oxygen and of carbon dioxide, exchange of chloride. Molecular basis of cooperativity of oxygen binding to hemoglobin. Hetero tropics allosteric effectors of hemoglobin. Hemoglobin variants and pathological hemoglobins.

CARBOHYDRATES
General Properties. Classification. Isomerism and stereoisomerism. Monosaccharides, disaccharides, oligosaccharides, homo- and hetero polysaccharides. Glycoproteins. Proteoglycans.

LIPIDS
General Properties. Classification. Saturated and unsaturated fatty acids. Essential fatty acids. Triglycerides. Phospholipids. Glycolipids. Prostaglandins. Sterols and steroids. Bile acids. Lipoproteins.

NUCLEIC ACIDS
Nucleobases, nucleosides, nucleotides. DNA, RNA. DNA replication. Protein- nucleic acids interaction. Allosteric proteins in the regulation of gene expression. The PCR and its main applications in biotechnology and diagnostics.

VITAMINS
Overview, classification, structure and function. Vitamin A. Vitamin D. Vitamin E. Vitamin K. B vitamins: thiamine, riboflavin, pantothenic acid, nicotinamide, piridossine, biotin. Antianemic vitamins: folic acid and cobalamin. Vitamin C. For each vitamin: chemistry, mechanism of action, metabolism, distribution, human needs, vitamin deficiency.
ELEMENTS OF BIOCHEMICAL METHODS
Chromatography. Electrophoresis. Protein and nucleic acid spectroscopy. X-ray crystallography



Module: BIOCHEMISTRY I - APPLIED BIOLOGY
N/D
Module: BIOCHEMISTRY II - BIOCHEMISTRY
N/D
Module: BIOCHEMISTRY II - MOLECULAR BIOLOGY
Programma Inglese:
Gene therapy approaches to disease treatment.
Main in vivo and ex vivo gene transfer techniques; viral and non-viral vectors.
Engineered proteins; RNA-based therapies.
Transgenesis; gene targeting; CRISPR/Cas approaches.

Module: BIOCHEMISTRY I - BIOCHEMISTRY
N/D
Module: BIOCHEMISTRY I - MOLECULAR BIOLOGY
-Jeremy M Berg, John L Tymoczko, Lubert Stryer. Biochimica. Ottava edizione -2020-Zanichelli Editore.
-Garrett - Grisham. Biochimica. Data di pubblicazione: febbraio 2014 - Piccin Editore.
-Mathews. Biochimica. Data di pubblicazione: ottobre 2014 - Piccin Editore.
-David L Nelson, Michael M Cox. I principi di biochimica di Lehninger. Settima edizione - 2018 - Zanichelli Editore.
-Siliprandi e Tettamanti. Biochimica Medica strutturale, metabolica e funzionale. Quinta edizione- 2018- Piccin Editore.
-Donald Voet, Judith G Voet, Charlotte W Pratt. Fondamenti di biochimica. Quarta edizione italiana condotta sulla quinta edizione americana - 2017 - Zanichelli.



Module: BIOCHEMISTRY I - APPLIED BIOLOGY
N/D
Module: BIOCHEMISTRY II - BIOCHEMISTRY
N/D
Module: BIOCHEMISTRY II - MOLECULAR BIOLOGY
“Genetica e genomica nelle scienze mediche” di Tom Strachan, Anneke Lucassen-Seconda edizione Zanichelli 2024

Module: BIOCHEMISTRY I - BIOCHEMISTRY
N/D
Module: BIOCHEMISTRY I - MOLECULAR BIOLOGY
N/D
Module: BIOCHEMISTRY I - APPLIED BIOLOGY
N/D
Module: BIOCHEMISTRY II - BIOCHEMISTRY
N/D
Module: BIOCHEMISTRY II - MOLECULAR BIOLOGY
N/D

Module: BIOCHEMISTRY I - BIOCHEMISTRY
N/D
Module: BIOCHEMISTRY I - MOLECULAR BIOLOGY
N/D
Module: BIOCHEMISTRY I - APPLIED BIOLOGY
N/D
Module: BIOCHEMISTRY II - BIOCHEMISTRY
N/D
Module: BIOCHEMISTRY II - MOLECULAR BIOLOGY
N/D

Module: BIOCHEMISTRY I - BIOCHEMISTRY
N/D
Module: BIOCHEMISTRY I - MOLECULAR BIOLOGY
N/D
Module: BIOCHEMISTRY I - APPLIED BIOLOGY
N/D
Module: BIOCHEMISTRY II - BIOCHEMISTRY
N/D
Module: BIOCHEMISTRY II - MOLECULAR BIOLOGY
N/D

Module: BIOCHEMISTRY I - BIOCHEMISTRY
N/D
Module: BIOCHEMISTRY I - MOLECULAR BIOLOGY
N/D
Module: BIOCHEMISTRY I - APPLIED BIOLOGY
N/D
Module: BIOCHEMISTRY II - BIOCHEMISTRY
N/D
Module: BIOCHEMISTRY II - MOLECULAR BIOLOGY
N/D

Module: BIOCHEMISTRY I - BIOCHEMISTRY
N/D
Module: BIOCHEMISTRY I - MOLECULAR BIOLOGY
N/D
Module: BIOCHEMISTRY I - APPLIED BIOLOGY
N/D
Module: BIOCHEMISTRY II - BIOCHEMISTRY
N/D
Module: BIOCHEMISTRY II - MOLECULAR BIOLOGY
N/D

Module: BIOCHEMISTRY I - BIOCHEMISTRY
N/D
Module: BIOCHEMISTRY I - MOLECULAR BIOLOGY



Module: BIOCHEMISTRY I - APPLIED BIOLOGY
N/D
Module: BIOCHEMISTRY II - BIOCHEMISTRY
N/D
Module: BIOCHEMISTRY II - MOLECULAR BIOLOGY

• Teaching Unit 1:- Gene therapy approaches to disease treatment.- Main in vivo and ex vivo gene transfer techniques; viral and non-viral vectors.
  • Books: “Genetica e genomica nelle scienze mediche” di Tom Strachan, Anneke Lucassen-Seconda edizione Zanichelli 2024


• Teaching Unit 2:- Engineered proteins; RNA-based therapies. 
  • Books: “Genetica e genomica nelle scienze mediche” di Tom Strachan, Anneke Lucassen-Seconda edizione Zanichelli 2024


• Teaching Unit 3:- Transgenesis; gene targeting in embryonic stem cells; CRISPR/Cas approaches. 
  • Books: “Genetica e genomica nelle scienze mediche” di Tom Strachan, Anneke Lucassen-Seconda edizione Zanichelli 2024