Medicine and Surgery

Unit 1 - Technologies for studying genetic variability and their applications (4 hours) Methods for the molecular study of variants Molecular analysis of gene mutations Unit 2 - Methodologies and experimental approaches for studying epigenetic variations (4 hours) Overview of methods for analyzing DNA methylation, histone modifications, ncRNA expression, ncRNA-protein binding, and ncRNA binding to chromatin Unit 3 - Techniques for modifying the epigenome (2h) Epigenetic enzyme inhibitors. RNA interference for epigenetic enzymes. CRISPR/Cas9 technology for inducing site-specific methylation Unit 4 - “Omic” approaches to the study of genetic variability and gene expression (4h) “Omic” technologies and systems biology. Genomics and genome sequencing Functional genomics: transcriptomics, proteomics, epigenomics, and metabolomics Unit 5 - Analysis of genetic variability in relation to drug susceptibility (2 hours) Molecular basis of drug variability. Genomic analysis and pharmacogenetic testing. Epigenetic and non-coding aspects. Prospects and personalized medicine Unit 6 - Genetic approaches to disease treatment (4 hours) Replacement gene therapy. Genome editing (CRISPR-Cas9 and next-generation technologies). RNA-based therapies. Epigenetic therapies. CAR-T therapies and immune system engineering. Mitochondrial therapies. Unit 7 - In-depth study of the methodologies covered (4 hours) Simulations of theoretical questions, possible practical applications, and self-assessment tests to consolidate skills.

1) Russell - Genetica. Un approccio molecolare- Pearson 2) Strachan e Lucassen - Genetica e Genomica nelle scienze mediche - Zanichelli Teaching materials provided by lecturers