Medicine and Surgery

Variability in laboratory medicine and quality controls. How to analyze a sample. Main analytical methods. Calibration curve and calibration materials. Appropriateness in Laboratory Medicine. Correct diagnostic procedure. Knowing how to request a laboratory investigation: Why request a laboratory exam: paper request; LIS (Laboratory Information System). Variability in Laboratory Medicine: Analytical and Biological variability. Sample collection. Peripheral venous blood drawing. Choice and use of the test tubes. Criteria for sample acceptability. Reliability criteria of a laboratory survey. Precision, accuracy, sensitivity and analytical specificity. Errors in Laboratory Medicine. The intra and inter laboratory quality checks. Knowing how to interpret a laboratory survey: Chronobiological variability; Index of individuality; Reference intervals, critical values and decision limits; Longitudinal and transversal comparison and critical difference. How to evaluate a diagnostic test: Prevalence, sensitivity and diagnostic specificity; Diagnostic efficacy and positive and negative predictive values; ROC Curves.

Before attending the lessons of the course, students must have acquired a solid basic knowledge of Statistics, Chemistry, Biochemistry and Microbiology. He must also know the rudiments of the main laboratory methods applicable to the qualitative and quantitative study of the constituents of biological matrices and those of the biological processes that represent the basis of knowledge in medicine. Furthermore, in particular for the lessons of the second semester, students must have acquired fundamental knowledge of Anatomy, Physiology, General Pathology and Pathophysiology.

Ciaccio. Trattatato di Biochimica Clinica e Medicina di Laboratorio. Edizioni Edises.

Lesson in presence or in telematic mode on Zoom platform. Projection and commentary of power point file concerning specific topics indicated in the program of exam.

Students must have attended at least 66% of the lessons of the Course

Oral exam with questions on topics listed in the exam program, possible a written test on the topics of the program through quizzes with multiple choice answers. The written test aims to evaluate the student's preparation on the entire exam program in a "punctual" way; the oral test, always present, intends to evaluate in depth the student's knowledge on some topics of the program, at the same time the language properties in explaining topics and the way in which the exposure is organized will be evaluated.

Ciaccio, Lippi. Biochimica Clinica e Medicina di Laboratorio. Edizioni Edises.

Lesson in presence or in telematic mode on Zoom platform. Projection and commentary of power point file concerning specific topics indicated in the program of exam.