Cell biology and technology

General Skills The course aims to study the structural and functional organization of the plant cell and the models of plant development, using experimental systems and advanced methodologies. It also aims to provide the knowledge and technological tools to increase the professionalism of the biologist in sectors that use plant cell systems. The course requires knowledge of plant Botany and Physiology acquired in the three-year degree in Biology. The course includes lectures. Specific skills A) Knowledge and understanding - Knowledge and understanding of the structural and functional organization of the different cellular compartments, peculiar of the plant cell. - Knowledge and understanding of molecular mechanisms in plant plasticity and development. - Knowledge and understanding of experimental systems used as model systems to study the biology of plant development B) Applying knowledge and understanding - be able to use the specific terminology of the subject - be able to identify the right procedures to solve the questions of cell biology and plant development C) Making judgements - to acquire critical judgment skills in the reading of experimental procedures and results reported in the literature on specific research issues - to learn to ask questions for the elaboration and deepening of the knowledge learned D) Communication skills - be able to communicate during the oral exam what has been learned during the lectures and the laboratory experiences E) Learning skills - learning specific terminology of the matter - be able to logically connect the acquired knowledge - be able to identify the most relevant topics of the course. COURSE PROGRAM: Part I: Biogenesis and ultrastructural characteristics of the peculiar compartments of plant cells. Cell wall. Vacuole. Plastids. Cytoskeleton. Endomembrane system. Nucleus and genome: the cell cycle and its regulation in the coordination between proliferation and differentiation. Cellular networks. Programmed cell death. Cell growth and differentiation. Polyploidization during differentiation and consequences for development. Part II: Definition of totipotency and pluripotency of plant cells. Stem cells and their localization in meristems. Plasticity and determination of cellular destination. Initial and derived cells. Cellular identity markers. Functional coordination in the meristem and mechanisms of interdependence between meristems. The position effect in differentiation. Mechanisms for defining the plant organization plan. Modulation of post-embryonic development in stem, root and leaf. Floral transition and flower development. Reprogramming and transdifferentiation. Multifunctional tissues in the different organs of the plant. Main experimental systems for the study of plant cell differentiation and industrial applications. Adventitious rooting and its applications. Somatic embryogenesis.

The course requires basic knowledge of Organic Chemistry, Biochemistry, Botany, Plant Physiology and Molecular Biology, acquired in the three-year course

Parte 1: Biologia Cellulare e Molecolare delle Piante. Editore PICCIN Parte 2: Elementi di biologia dello sviluppo delle piante. Editore EdiSES

Attendance at classroom lessons is not mandatory, but highly recommended

The assessment will be carried out with a single oral exame at the end of the lessons. The student will be able to exhibit the knowledge gained during the lessons and during the own study. The oral exam can be performed at the student's choice in one of the 5 exam sessions. For out-of-school students there are 2 other sessions.

The course includes lectures. Through the lectures students learn the fundamental knowledge of the discipline. The lessons are held twice a week in the classroom and the exposure takes place through the use of slides on power-point. There are tests of self-assessment in progress, there will be in the classroom the opportunity to review together with the teacher key aspects of the program played and deepen some topics chosen by the student who will present in the form of power point.

General Skills The course aims to study the structural and functional organization of the plant cell and the models of plant development, using experimental systems and advanced methodologies. It also aims to provide the knowledge and technological tools to increase the professionalism of the biologist in sectors that use plant cell systems. The course requires knowledge of plant Botany and Physiology acquired in the three-year degree in Biology. The course includes lectures. Specific skills A) Knowledge and understanding - Knowledge and understanding of the structural and functional organization of the different cellular compartments, peculiar of the plant cell. - Knowledge and understanding of molecular mechanisms in plant plasticity and development. - Knowledge and understanding of experimental systems used as model systems to study the biology of plant development B) Applying knowledge and understanding - be able to use the specific terminology of the subject - be able to identify the right procedures to solve the questions of cell biology and plant development C) Making judgements - to acquire critical judgment skills in the reading of experimental procedures and results reported in the literature on specific research issues - to learn to ask questions for the elaboration and deepening of the knowledge learned D) Communication skills - be able to communicate during the oral exam what has been learned during the lectures and the laboratory experiences E) Learning skills - learning specific terminology of the matter - be able to logically connect the acquired knowledge - be able to identify the most relevant topics of the course. COURSE PROGRAM: Part I: Biogenesis and ultrastructural characteristics of the peculiar compartments of plant cells. Cell wall. Vacuole. Plastids. Cytoskeleton. Endomembrane system. Nucleus and genome: the cell cycle and its regulation in the coordination between proliferation and differentiation. Cellular networks. Programmed cell death. Cell growth and differentiation. Polyploidization during differentiation and consequences for development. Part II: Definition of totipotency and pluripotency of plant cells. Stem cells and their localization in meristems. Plasticity and determination of cellular destination. Initial and derived cells. Cellular identity markers. Functional coordination in the meristem and mechanisms of interdependence between meristems. The position effect in differentiation. Mechanisms for defining the plant organization plan. Modulation of post-embryonic development in stem, root and leaf. Floral transition and flower development. Reprogramming and transdifferentiation. Multifunctional tissues in the different organs of the plant. Main experimental systems for the study of plant cell differentiation and industrial applications. Adventitious rooting and its applications. Somatic embryogenesis.

The course requires basic knowledge of Organic Chemistry, Biochemistry, Botany, Plant Physiology and Molecular Biology, acquired in the three-year course

Parte 1: Biologia Cellulare e Molecolare delle Piante. Editore PICCIN Parte 2: Elementi di biologia dello sviluppo delle piante. Editore EdiSES

Attendance at classroom lessons is not mandatory, but highly recommended

The assessment will be carried out with a single oral exame at the end of the lessons. The student will be able to exhibit the knowledge gained during the lessons and during the own study. The oral exam can be performed at the student's choice in one of the 5 exam sessions. For out-of-school students there are 2 other sessions.

The course includes lectures. Through the lectures students learn the fundamental knowledge of the discipline. The lessons are held twice a week in the classroom and the exposure takes place through the use of slides on power-point. There are tests of self-assessment in progress, there will be in the classroom the opportunity to review together with the teacher key aspects of the program played and deepen some topics chosen by the student who will present in the form of power point.