BIOCHEMICAL BIOTECHNOLOGIES Single channel
Chair (Coordinator) and Rapporteur: MARIA CARMELA BONACCORSI DI PATTI
Module 1: MICROBIAL BIOTECHNOLOGIES: INDUSTRIAL APPLICATIONS
- Activity type
- Attività formative affini o integrative
- SSD
- BIOS-07/A
- Year
- 1st year
- Semester
- 2nd semester
- CFU
- 3
- Hours distribution
- 24 classroom hours
- Lecturers
- MARIA CARMELA BONACCORSI DI PATTI
Module 2: APPLIED BIOCHEMISTRY
- Activity type
- Attività formative affini o integrative
- SSD
- CHEM-07/C
- Year
- 1st year
- Semester
- 2nd semester
- CFU
- 3
- Hours distribution
- 24 classroom hours
- Lecturers
- CRISTINA MAZZONI
Objectives
The course of "Biochemical Biotechnologies " consists of the integrated modules "Applied Biochemistry" and "Microbial Biotechnologies: Industrial Applications". The learning outcomes of the course concern biotechnological applications of enzymes, proteins and microorganisms in basic and applied research and in industry. The learning outcomes of each individual module can be consulted in the appropriate section of the module.
Learning outcomes
The course of "Biochemical Biotechnologies " consists of the integrated modules "Applied Biochemistry" and "Microbial Biotechnologies: Industrial Applications". The learning outcomes of the course concern biotechnological applications of enzymes, proteins and microorganisms in basic and applied research and in industry. The learning outcomes of each individual module can be consulted in the appropriate section of the module.
Prerequisites
The course of Biochemical Biotechnologies in the master course is in the first year. Basic knowledge of Cell Biology, Microbiology, Genetics, Biochemistry and Molecular Biology is required.
Programme
Module: MICROBIAL BIOTECHNOLOGIES: INDUSTRIAL APPLICATIONS
The course includes 20 hours of classroom lectures and 4 hours of guided exercises.
Biotechnological applications of enzymes. Production of recombinant enzymes and proteins: methods and strategies. Protein engineering: site-specific mutagenesis,
in vitro evolution. Production of therapeutic proteins (hormones, antibodies, coagulation factors). Main techniques of cell and enzyme immobilization. Biocatalysts: use of enzymes for production of biotechnologically relevant compounds.
Multienzymatic systems for biosynthesis of bioactive secondary metabolites. Non-ribosomal biosynthesis of peptides. Enzyme mechanism of peptide synthetases. Examples (gramicidin, surfactin, penicillins and cefalosporins). Biosynthesis of polyketides. Mechanism of polyketide formation and analogies with fatty acid biosynthesis. Examples (erythromycin, statins).
Module: APPLIED BIOCHEMISTRY
N/D
Books
Module: MICROBIAL BIOTECHNOLOGIES: INDUSTRIAL APPLICATIONS
There is no specific text book. Teaching materials (slides of the lessons and scientific papers) are published on the educational site Elearning2 at http://elearning2.uniroma1.it
Module: APPLIED BIOCHEMISTRY
N/D
Bibliography
Module: MICROBIAL BIOTECHNOLOGIES: INDUSTRIAL APPLICATIONS
N/D
Module: APPLIED BIOCHEMISTRY
N/D
Lessons mode
classroom lectures and small group activities
Frequency
attendance is not mandatory but strongly advised
Exam mode
oral presentation of a paper from the scientific literature on topics of the course program
Example exam questions
The questions will focus on the specific scientific work presented. Much emphasis will be given to the following criteria:
Description of the background and rationale of the work
Description of the experimental setup
Techniques used and for what purpose
Discussion of the results and possible perspectives
Arguments
Module: MICROBIAL BIOTECHNOLOGIES: INDUSTRIAL APPLICATIONS
- applied biochemistry
- microbial biotechnologies
Module: APPLIED BIOCHEMISTRY
- applied biochemistry
- microbial biotechnologies
- Academic year2026/2027
- Degree program to which the course belongsGenetics and Molecular Biology
- Mandatory presenceNo
- LanguageENG
- CFU6 CFU, distributed among 2 integrated didactic modules
- Total duration48 hours