Catalogo dei Corsi di studio

Name and address of the Institution: Department of Medical-surgical Science and Biotechnologies, University Sapienza, Faculty of Medicine, Via Corso della Repubblica 79, Latina 04100 Italy
Phone: lab +39 07731757242- fax +39 07731757254

EDUCATION

Title: PhD in Biochemistry
Date of Achievement: 03/2001
Name and address of the Institution: University La Sapienza di Roma, Piazzale Aldo Moro 5, 00185 Rome

Title: School in Biotechnology
Date of Achievement:17/03/1997
Name and address of the Institution: Universita' of Rome La Sapienza, Piazzale Aldo Moro 5, 00185 Rome Mark:70/70 e lode.
Award thesis in Biotechnology 1995-1997

Title: Biological Sciences Degree (summa cum laude)
Date of Achievement:1991
Name and address of the Institution: University La Sapienza Rome, Piazzale Aldo Moro 5, 00185 Rome.
ENEA/INN-BIO-AG Via Anguillarese Rome.

Native language: Italian
Other languages: English

Title: Felasa B (Animal Laboratory Science)
Date of Achievement: 15/06/2020
Name and address of the Institution: VIB-KU Leuven Center for Cancer Biology O&N4, 9e verd Campus Gasthuisberg Herestraat 49 3000 Leuven, Belgium.

EXPERIENCES

Period: from 02/09/2019 to 30/06/2021
Position: Visiting Scientist at VIB-KU Leuven Center for Cancer Biology O&N4, 9e verd Campus Gasthuisberg Herestraat 49 3000 Leuven, Belgium.

Period: from 01/ 05/2017 to 31/07/2017
Position: Guest Scientist at the Heidelberg University, Medical Faculty, Department of Anatomy and Cell Biology, Heidelberg Germany. Supported by funding of the German Academic Exchange Service (DAAD fellow personal nf. No 91609354 section ST24) Funding programme/-ID Research stays for University Academics and Scientists, 2017 (57314018).

Period: from 31/12/2010
Position: Researcher (SSD MED04) with permanent position in the Laboratory of Molecular Oncology
Institute: Department of Medico-surgical Science and Biotechnologies, University Sapienza, Faculty of Medicine, Corso della Repubblica 79, Latina 04100. Italy.

Period: from 04/2010 to 12/10
Name and address of the Institution: University of Rome, Corso della Repubblica 79, 04100 Latina, Italy
Position: Post-doc position, Laboratory of Molecular Oncology
Institute: Department of Experimental Medicine and Pathology. University Sapienza

Period: 09/2008 to 06/2009
Name and address of Institution: Istituto Campagnano, via B. Lesen 2, Rome Italy
Position: Math and Science Teacher

Period: from 04/02 to 05/08
Name and address of the Institution: University of Rome, Piazzale Aldo Moro 5 00185 Roma Italy
Position: Research Fellow, Fellowship from Cenci Bolognetti Foundation
Institute: Department of Biotechnology and Hematology.

Period: from 03/95 to 02/02
Name and address of the Institution: University of Rome, La Sapienza Piazzale Aldo Moro 5, 00185 Rome Italy
Position: fellowship from CNR, fellowship from Cenci Bolognetti Foundation, PhD fellowship
Institute: Department of Biochemistry.

Period: from 03/91 to 02/95
Name and address of the Institution: Enea, via Anguillarese Rome Italy
Position: fellowship from CNR
Institute: Department of Biotechnology

Supervisor of the following students:
1. Maria Troiano. PhD in Human Biology and Genetics Department of Biotechnology and Hematology, University Sapienza, Rome (2007)
2. Luca Soscia. Degree in Tecniche di laboratorio Biomedico. Department Medical-surgical Science and Biotechnology, University Sapienza Latina (2015)
3. Marzia Tagliaferro. Degree in Tecniche di laboratorio Biomedico. Department of Medical-Surgical Science and Biotechnology University Sapienza, Latina (2016/2017)
4. Marzia Tagliaferro Degree in Biotechnology. Department of Medical-Surgical Science and Biotechnology, University of Rome Sapienza, Latina (2018/2020)
Member of:
- Ethics committee of Department of Medical-Surgical Sciences and Biotechnology since 2017
- Albo Nazionale dei Biologi since 2008
- Albo Nazionale dei Revisori CINECA, MIUR since 2011
- Italian Association of the book since 2015

TEACHING:

Coordinator of the Course Molecular and Cellular Basis of the Life (BIO13/MED03/FIS07/BIO10) from 2013 to now. Cod. 1034944 -Faculty of Medicine, University Sapienza.

- Biology (BIO13) and Genetics (MED03) from 2013 to 2014 (36h/year) and Biology (BIO13) from 2015 to now (24h/year), Cod. 1034944-Faculty of Medicine, University Sapienza.
- Biology (BIO13) and Genetics (MED03) from 2012 to now (36h/year) cod.1035043-Faculty of Medicine, University Sapienza.
- Molecular Pathology (MED04) from 2011 to 2013-2CFU/year (24h/year) (cod. 16011) -Faculty of Medicine, University Sapienza.
- Molecular Pathology and Physiopathology (MED04) from 2014 to now (24h/year) cod. 16011 -Faculty of Medicine, University Sapienza.
PERSONAL SKILL
Molecular Biology: site directed mutagenesis, DNA manipolation (cloning, PCR, sequencing) Northern and Southern blotting, RT-PCR, Race 5', Race 3'. Screening of cDNA and genomic library, library, Transcription and Translation in-vitro. Biochemistry: electrophoresis of proteins and peptides, protein purification, immunoblotting, immunoprecipitation, ChIP, EMSA. Sucrose and Cs gradient. Cell fractioning (nucleolar extraction). Real Time PCR, Analysis of methylation status of promoter gene. Extraction of nucleolar proteins. Heterologous expression: of proteins and peptides. Cell biology: cell culture, transfection, siRNA, immunofluorescence, adenovirus infection. Experimental model: amphibian; transgenic plant; transgenic D. melanogaster; cell lines; primary cells; mouse Egr1 -/-.
Since 2012 to 2015 she is allowed to the use of mouse model for the project: Ruolo del fattore di trascrizione Egr-1 nella contrazione cardiaca; regolazione dei processi molecolari che sottendono alla normale movimentazione del calcio citoplasmatico nei miocardiociti . (Research project Dlg 116/92. Dlg n.66/2011-A)

PUBBLICATIONS

-Nucleolar localization of the ErbB3 receptor as a new target in glioblastoma.
Tagliaferro M, Rosa P, Bellenchi GC, Bastianelli D, Trotta R, Tito C, Fazi F, Calogero A, Ponti D. BMC Mol Cell Biol. 2022 Mar 7;23(1):13. doi: 10.1186/s12860-022-00411-y.

- Nucleolar stress controls mutant Huntington toxicity and monitors Huntington's disease progression.
Sönmez A, Mustafa R, Ryll ST, Tuorto F, Wacheul L, Ponti D, Litke C, Hering T, Kojer K, Koch J, Pitzer C, Kirsch J, Neueder A, Kreiner G, Lafontaine DLJ, Orth M, Liss B, Parlato R. Cell Death Dis. 2021 Dec 8;12(12):1139. doi: 10.1038/s41419-021-04432-x. (IF 8.46)

-Manuel Ehling , Ward Celus , Rosa Martín-Pérez , Roser Alba-Rovira , Sander Willox , Donatella Ponti , Maria C Cid, Elizabeth A V Jones , Giusy Di Conza, Massimiliano Mazzone. (2020) B55 /PP2A Limits Endothelial Cell Apoptosis During Vascular Remodeling: A Complementary Approach To Disrupt Pathological Vessels? Circ Res 127(6):707-723. doi: 10.1161/CIRC. RES. AHA.119.316071. (IF 14.46)
- Mohsen Ibrahim, Davide Scozzi, Kelsey A Toth, Donatella Ponti, Daniel Kreisel, Cecilia Menna, Elena De Falco, Antonio D Andrilli, Erino A. Rendina, Antonella Calogero, Alexander S. Krupnick, and Andrew E. Gelman (2018) Naïve CD4+ T cells carrying a TLR2 agonist overcome TGFbeta-mediated tumor immune evasion. The Journal of Immunology 200:847-956) (IF 4.8)

- Mincione Gabriella, Maria C. Di Marcantonio, Chiara Tarantelli, Luca Savino, Donatella Ponti, Marco Marchisio, Paola Lanuti, Silvia Sancilio, Antonella Calogero, Roberta Di Pietro, Raffaella Muraro (2016) Identification of the Zinc Finger 216 (ZNF216) in human carcinoma cells: a potential regulator of EGFR activity. Oncotarget 7:74947-74965 (IF 6.3)

- Donatella Ponti, Daniela Bastianelli, Paolo Rosa, Luca Pacini, Mohsen Ibrahim, Erino Angelo Rendina, Giuseppe Ragona, Antonella Calogero (2015) The transcriptional factor Egr1 promotes B23 expression under stress conditions (BMC Cell Biology 16:27. doi: 10.1186/s12860-015-0073-5). (IF 2.34)
- Camilla Siciliano, Isotta Chimenti, Antonella Bordin, Donatella Ponti, Paola Iudicone, Mariangela Peruzzi, Erino Angelo Rendina, Antonella Calogero, Luca Pierelli , Mohsen Ibrahim, Elena De Falco (2015) The Potential of GMP-Compliant Platelet Lysate to Induce a Permissive State for Cardiovascular Transdifferentiation in Human Mediastinal Adipose Tissue-Derived Mesenchymal Stem Cells BioMed Research International 01/2015. (IF 2.7)
- Pacini Luca, Bastianelli Daniela, Ponti Donatella, Rosa Paolo, Petrozza Vincenzo, Giannini Giuseppe, Ragona Giuseppe, Calogero Antonella. (2015) Direct correlation between double K-RAS mutation and mucinous carcinoma. Case report. Appl Immunohistochem Mol Morphol 23(2):e4-7. doi: 10.1097/PAI.0000000000000176 (IF 2)
- Luca Pacini, Elena De Falco, Maria Di Bari, Andrea Coccia, Camilla Siciliano, Donatella Ponti, Antonio Pastore, Vincenzo Petrozza, Antonio Carbone, Ada Tata, Antonella Calogero (2014) M2 muscarinic receptors inhibit cell proliferation and migration in urothelial bladder cancer cells. Cancer Biol Ther. 2014 Aug 22:0. PMID:25482946 (IF 3)
- Donatella Ponti, Gian Carlo Bellenchi, Rosa Puca, Daniela Bastianelli, Marella Maroder, Giuseppe Ragona, Pascal Roussel, Marc Thiry, Dan Mercola, Antonella Calogero (2014). The Transcription Factor EGR1 Localizes to the Nucleolus and Is Linked to Suppression of Ribosomal Precursor Synthesis. PLOS ONE, vol. 9, ISSN: 1932-6203, doi: 10.1371/journal.pone.0096037. (IF 3.2)

- Luca Pacini, Silvia Suffredini, Donatella Ponti, Raffaele Coppini, Giacomo Frati, Giuseppe Ragona, Elisabetta Cerbai, Antonella Calogero (2013). Altered calcium regulation in isolated cardiomyocytes from Egr-1 knock-out mice. Canadian Journal of Physiology and Pharmacology, ISSN: 0008-4212, doi: 10.1139/cjpp-2012-0419. (IF 1.7)

- Michela Ferretti, Cinzia Fabbiano, Maria Di Bari, Claudia Conte, Emilia Castigli, Miriam Sciaccaluga, Donatella Ponti, Paola Ruggieri, Antonino Raco, Ruggero Ricordy, Antonella Calogero, Ada Maria Tata (2013). M2 receptor activation inhibits cell cycle progression and survival in human glioblastoma cells. Journal of Cellular and Molecular Medicine, vol. 17, p. 552-566, ISSN: 1582-1838, doi: 10.1111/jcmm.12038. (IF 4)

- Daniele Avitabile, Licia Genovese, Donatella Ponti, D. Ranieri, S. Raffa, Antonella Calogero, Maria R. Torrisi (2013). Nucleolar localization and circadian regulation of Per2S, a novel splicing variant of the Period 2 gene. Cellular and Molecular Life Sciences, ISSN: 1420-9071. (IF 5.8)

- M. Ferretti, C. Fabbiano, M. Di Bari, D. Ponti, A. Calogero, A.M. Tata (2012). M2 muscarinic receptors inhibit cell proliferation in human glioblastoma cell lines. Life Sciences, ISSN: 0024-3205, doi: 10.1016/j.lfs.2012.04.033. (IF 2.7)

- Paola Ruggieri, Giorgio Mangino, Bernard Fioretti, Luigi Catacuzzeno, Rosa Puca, Donatella Ponti, Massimo Miscusi, Fabio Franciolini, Giuseppe Ragona, Antonella Calogero (2012). The Inhibition of KCa3.1 Channels Activity Reduces Cell Motility in Glioblastoma Derived Cancer Stem Cells. PLOS ONE, vol. 7, p. 1-12, ISSN: 1932-6203, doi: 10.1371/journal.pone.0047825. (IF 3.2)

- Calogero A, Porcellini A, Lombari V, Fabbiano C, Arcella A, Miscusi M, Ponti D, Ragona G. (2011). Sensitivity to cisplatin in primary cell lines derived from human glioma correlates with levels of EGR-1 expression. Cancer Cell International, vol. 5, ISSN: 1475-2867, doi: 10.1186/1475-2867-11-5. (IF 2.7)

- Psaila Rossana, Donatella Ponti, Ponzi Marta, Gigliani Franca, Battaglia Piero Augusto (2010). A small sequence in domain V of the mitochondrial large ribosomal RNA restores Drosophila melanogaster pole cell determination in UV-irradiated embryos. Cellular & Molecular Biology Letters, ISSN: 1425-8153, doi: 10.2478/s11658-010-0013-5. IF 1.6

- Donatella Ponti, Maria Troiano, Gian Carlo Bellenchi, Piero Augusto Battaglia, Franca Gigliani (2008) The HIV Tat protein affects processing of ribosomal RNA precursor. BMC Cell Biology 9:32. (IF 2.34)
- Piero A. Battaglia; Ponti Donatella; Gigliani Franca (2006) Two phenotypes with a single genotype: the case of butterfly. Caryologia Volume: 59 Issue: 4 Pages: 354-357 (IF 0.45)

- Piero A Battaglia, Donatella Ponti, Valeria Naim, Serenella Venanzi, Rossana Psaila, Franca Gigliani (2005) The HIV-Tat protein induces chromosome number aberrations by affecting mitosis. Cell Motility and the Cytoskeleton 61:129-36. (IF 4.1)

-Donatella Ponti, Maria Luisa Mangoni, Giuseppina Mignogna, Maurizio Simmaco and Donatella Barra (2003) "An amphibian antimicrobial peptide expressed in Nicotiana tabacum confers plant resistance to phytopathogens" Biochemical Journal 370,121-7 (IF 5)

-Donatella Ponti, Giuseppina Mignogna, Maria Luisa Mangoni, Daniela De Biase, Maurizio Simmaco and Donatella Barra (1999) Expression and activity of cyclic and linear analogues of esculentin-1, an antimicrobial peptide from amphibian skin. European Journal Biochemistry, 263, 921-927 (IF 3.16)

-Rossella Miele, Donatella Ponti, Hans G. Boman, Donatella Barra and Maurizio Simmaco (1998) A tandem gene coding for bombinins, antibacterial peptides from the frog Bombinina orientalis . FEBS Lett. 10;431: 23-8 (IF 3.6)

- Ugo Borello, Benedetto Aracri, Claudia Dalmastri, Donatella Farini, Donatella Ponti and Giovanni Giuliano (1993) Transcriptional Regulation of nuclear genes controlling plastid differentiation in tomato . Biotechnology Biotechnogical Equipment, 2:51-54 (IF 0.38)

-Giuliano Giuliano, Borello Ugo, Ponti Donatella. (1995) A B-ZIP factor-binding to the the G-Box of light-responsive genes in a phosphorylation-independent fashion. Journal of Cellular Biochemistry Supplement: 21A-504-504 (IF 3.12)
- Klimczak LJ, Farini D, Lin C, Ponti D, Cashmore AR, Giuliano G. (1995) Multiple isoforms of Arabidopsis casein kinase I combine conserved catalytic domains with variable carboxyl-terminal extensions. Plant Physiol ;109:687-96. (IF 6)
Patent
-Simmaco Maurizio, Barra Donatella, Ponti Donatella. (2001) Transgenic plant with increased resistance against phytopatogens and
methods of production. Italian patent no. RM2001A000268.
-Barra Donatella, Bossa Francesco, De Biase Daniela, Simmaco Maurizio & Ponti Donatella. (1998) Plasmid for the production of anti-microbial peptides in recombinant bacteria. Italian patent no. RM98A000393

Book

Russell, Peter J. iGenetica: fondamenti / Peter J. Russell; italian edition by Giancarlo Bellenchi, Franca Gigliani, Donatella Ponti Napoli: EdiSES, 2004 [opac SBN] [Monografia] [IT\ICCU\CAG\0762632]

Proceedings (2012-2018)

- Donatella Ponti Nucleolar variant of ErBB3 regulates RNA polymerase I activity FISV 2018 Rome Italy 18-21 September 2018

- Savino Luca, Di Marcantonio Maria Carmela, Tarantelli Chiara, Sancilio Silvia, Ponti Donatella, Muraro Raffaella, Mincione Gabriella. ZNF216 and EGFR mutual regulation in human carcinoma cells 29th Annual Conference of Italian Association of Cell Cultures (Onlus-Aicc) Crossroads in cellular and Molecular Biotechnology Novel Diagnostic and therapeutic strategies for Cancer, Immunology Disorders and rare Diseases L Aquila, 23-25 Novembre 2016

- Donatella Ponti, Daniela Bastianelli, Gian Carlo Bellenchi, Paolo Rosa, Rosa Puca, Giuseppe Ragona, Antonella Calogero. The transcription factor EGR1 regulates the expression of nucleolar protein B23. FISV 2014 Pisa, Italy
- Mohsen Ibrahim, Kelsey Toth, Donatella Ponti, Antonella Calogero, Claudio Andreetti, Howard Huang, Daniel Kreisel, Sasha Kreisel, Erino Rendina, and Andrew Gelman. Pam3Cys4 internalization by naïve CD4+T cells accelerates t-bet promoter epigenetic modification and restrains TGF 1 expressing tumor growth (Meeting Abstract Supplement). The Journal of Immunology 2013, 190, 170.3. Honolulu, Hawaii (IF 5.36)

- Donatella Ponti, Gian Carlo Bellenchi, Rosa Puca, Marella Maroder, Giuseppe Ragona, Pascal Roussel, Antonella Calogero. The Transcription Factor EGR1 Localizes to the Nucleolus and Is Linked to Suppression of Ribosomal Precursor Synthesis. The EMBO meeting 2012 Nice, France

COURSE
Mouse Genetics; Models for Human Disease 16-19 March 2015 ICGBE Trieste Italy
EMBO Career activity: How to get your paper published 22.09.2012 Nice September

FUNDING
- Individual funding 2020 Sapienza. Erbb3 a new player in glioblastoma
- Individual funding 2018 ErbB3 AND NUCLEOLAR METABOLISM - n. protocollo RP11816433CD0FDC
- Individual funding for research activity 2017 C.U.P. B25D17000260005 MIUR
- Academic funding 2017 Sapienza Role of the translation factor eIF6 in ribosome biogenesis and differential translation of specific mRNAs (Partecipant)
- Academic Funding 2016 Sapienza ErbB3: a new player of nucleolar metabolism (Principal investigator).
- Academic Funding 2015 Sapienza Crosstalk between B23/ARF/EGR1 nucleolar network and ErbB3 receptor in glioma cells code number C26A15HF29 (Principal investigator).
- Academic Funding 2014 Sapienza - Prot. n. 0067282 del 20.11.2014. EGR1/ARF/B23 nucleolar network in glioma cells code number C26A14WBCS (Principal investigator).
- Academic Funding 2013 Sapienza code number C26A139S3N Can Egr1 influence growth by controlling ribosomal metabolism? (Partecipant)
- Academic Funding 2012 Sapienza code number C26A12T3C4 Glioma stem Cells and KCa3.1 channel (Partecipant)
- Academic Funding 2011 Sapienza code number C26A11TAF Role of nucleolar compartment in gliomas (Participant)
-PRIN 2011 (Programmi di Ricerca di Interesse Nazionale) Prot. 2010 ZESJWN_007 (Participant)