Fiorentina.Ascenzioni@uniroma1.it's picture

Gli insegnamenti impartiti dalla Prof.ssa F. Ascenzioni nel I semestre dell'AA 2020-22 si svolgeranno secondo quanto stabilito da Sapienza con lezioni in presenza e a distanza in diretta.

 

Microbiologia Generale (BAI), 6 CFU, orario ed aula consultabili https://corsidilaurea.uniroma1.it/it/corso/2021/31269/programmazione

Aula virtuale, https://meet.google.com/wov-aebs-jwj?hs=122&authuser=0.

Inizio del corso: 23 settembre

 

Genetica Molecolare dei Microrganismi (LM-BTC), 6 CFU, lunedì, mercoledì e venerdì, 11.00-13.00; Aula V via dei Marsi (Psicologia),

Aula virtuale, https://meet.google.com/sez-aedc-uiy?authuser=0

Inizio del corso: 22 settembre

 

Vettori Microbici e Applicazioni in Terapia Genica e Cellulare (BTC), 6 CFU, lunedì e mercoledì 16.00-18.00; Aula V via dei Marsi

Aula virtuale, https://meet.google.com/gej-ioev-orj?hs=122&authuser=0

Inizio del corso: 29 settembre

 

Tirocinio (3 CFU), LM-BTC. Le attività di tirocinio, documentate all'atto della verbalizzazione che avverrà attraverso infostud; gli appelli sono presenti su infostud. In E-learning sono riportate le modalità di rendicontazione.

La Prof.ssa Ascenzioni riceve il mercoledì, dalle ore 14.30-16.30, presso la sede San Lorenzo del Dip BBCD, via dei sardi 70, I piano. Gli studenti sono invitati a contattare la Prof.ssa per mail (fiorentina.ascenzioni@un iroma1.it) anche per valutare la possibilità di un incontro a distanza.

Fiorentina Ascenzioni, CV, It
Occupazione e ricerca
Dal 2001, Professore associato di Microbiologia Generale, Sapienza Università di Roma, Dipartimento di Biologia e Biotecnologie C. Darwin . Nel marzo 2017 ha conseguito la qualifica nazionale professore ordinario in Microbiologia SSD BIO / 19 (SD 05 / I2).
Ricercatore e ricercatore associato presso l'Università di Roma La Sapienza (1991-2001). Visiting Professor presso Ecole Norma le Superieure de Lyon, Fr (1998-99).
Carichi didattici: Microbiologia Generale (Biotecnologie Agro Industriali, BAI, L3); Genetica Molecolare dei Microrganismi (Laurea Magistrale in Biologia e Tecnologie Cellulari, BTC); Vettori Microbici per Terapia Genica e Cellulare (Laurea Magistrale in Biologia e Tecnologie Cellulari, BTC)); Microbiologia ambientale (LM, Ecobiologia).
LM BTC, presidente (da novembre 2019);
Docente del Dottorato di ricerca in Biologia cellulare e dello sviluppo, Università degli Studi di Roma La Sapienza
Attività editoriale: revisore per riviste scientifiche (Frontiers in Microbiology, Nucleic Acids Research, Chromosome Research, Gene Therapy, Human Gene Therapy, J Gene Medicine, FEBS).
Valutazione di proetti di ricerca per Wellcome Trust (Regno Unito), ANR (Francia) e FWO (Belgio).
Membro di società scientifiche nazionali (SIMGBM, SIBBM e SIFC) e internazional (ECFS).
Premi: Miglior poster alla conferenza europea sulla fibrosi cistica, Brest (Fr) 2009; Proiezione di Annalisa Marzotto, congresso SIFC 2009

Principali interessi scientifici
I progetti di ricerca coordinati dalla Dott.ssa Ascenzioni sono i risultati della sua formazione scientifica nei seguenti settori: cromosomi artificiali eucariotici e procariotici; biologia molecolare e cellulare della fibrosi cistica (FC); microbiologia nella malattia polmonare FC
Gli argomenti principali sono:
Interazioni ospite-patogeno nella fibrosi cistica. L'obiettivo principale è quello di evidenziare i meccanismi molecolari alla base dell attività microbicida delle cellule fagocitiche e di valutarne la attività in relazione alla presenza di mutazioni nel gene CFTR.
Analisi della patogenicità dei ceppi di Pseudomonas aeruginosa e Burkholderia cepacea di origine clinica e ambientale. Più recentemente ci siamo concentrati sugli isolati di P. aeruginosa recuperati da pazienti con FC nelle ultime fasi della malattia polmonare per identificare le vie molecolari responsabili della virulenza batterica nella FC.
Nuove terapie per la malattia da infezione polmonare FC: sviluppo di farmaci mirati ai geni di resistenza alla colistina; identificazione di target per migliorare l'attività delle cellule fagocitiche CF, in particolare i macrofagi. I target includono canali ionici la cui attività appare alterata in FC come il canale ENaC; disregolazione di miRNA nei macrofagi FC
Sviluppo di cromosomi artificiali e vettori episomali per la consegna di geni terapeutici. I nostri studi prendono in considerazione la fibrosi cistica come malattia modello.

Fiorentina Ascenzioni, CV, En
Employment and Research Experience
Since 2001, Associate Professor in General Microbiology, Sapienza University of Rome, Department of Biology and Biotechnology C. Darwin . She obtained the national qualification full professor in Microbiology SSD BIO/19 (SD 05/I2)
Researcher and associate researcher at the University of Rome La Sapienza (1991-2001). Visiting Professor at Ecole Norma le Superieure de Lyon, Fr (1998-99).
Teaching: Microbiology (L3, BAI); Molecular Microbial Genetics (LM, BTC); Microbial Vectors for Cell and Gene Therapy (LM, BTC); Environmental Microbiology (LM, Ecobiologia).
LM BTC, President, since november 2019;
Mentor PhD in Cellular and Developmental Biology, University of Rome La Sapienza
Editorial Activity Referee for scientific Journal (Frontiers in Microbiology, Nucleic Acids Research, Chromosome Research, Gene Therapy, Human Gene Therapy, J Gene Medicine, FEBS).
Evaluation of research grant proposal for Wellcome Trust (UK), ANR (France) and FWO (Belgium).
Scientific Society Membership: national scientific society SIMGBM, SIBBM and SIFC; international, ECFS.
Prizes: Best poster at European cystic fibrosis conference, Brest (Fr) 2009; Annalisa Marzotto proze, SIFC congress 2009

Major scientific interests
The research projects coordinated by Dr. Ascenzioni are the results of her scientific formation in the following area: eukaryotic and prokaryotic artificial chromosomes; molecular and cellular biology of cystic fibrosis (CF); microbiology in CF lung disease.
Main topics are:
Host-pathogen interactions in cystic fibrosis. The main goal is to highlight the molecular mechanisms underlying the microbial killing by macrophages and to assess their activity in CF macrophages.
Analysis of the pathogenicity of Pseudomonas aeruginosa and Burkholderia cepacea strains of clinical and environmental origins. More recently we focused on P. aeruginosa isolates recovered from CF patients in the last stages of the lung disease to identify the molecular pathways responsible for bacterial virulence in CF.
New therapeutics for CF lung infection disease: development of drugs targeting the colistin resistance genes; identification of targets to improve the activity of CF immune cells, in particular macrophages. Targets include ion channels whose activity appear altered in CF such as the ENaC channel; miRNA dysregulation in CF macrophages.
Development of artificial chromosomes and episomal vectors for delivery of therapeutic genes. Our studies take into consideration Cystic Fibrosis as a model disease.

Course Code Year Course - Attendance
INTERNSHIP AAF1041 2021/2022 Cell biology and technology
GENERAL MICROBIOLOGY, MICROBIAL BIOTECHNOLOGIES AND ELEMENTS OF MEDICAL MICROBIOLOGY 1034848 2021/2022 Food and Industrial Biotechnology
MOLECULAR GENETICS OF MICROORGANISMS 1038169 2021/2022 Cell biology and technology
Microbial vectors and applications in gene and cell therapy 1020774 2021/2022 Cell biology and technology
INTERNSHIP AAF1041 2020/2021 Cell biology and technology
Microbial vectors and applications in gene and cell therapy 1020774 2020/2021 Cell biology and technology
GENERAL MICROBIOLOGY, MICROBIAL BIOTECHNOLOGIES AND ELEMENTS OF MEDICAL MICROBIOLOGY 1034848 2020/2021 Agro-Industrial Biotechnology
MOLECULAR GENETICS OF MICROORGANISMS 1038169 2020/2021 Cell biology and technology
MOLECULAR GENETICS OF MICROORGANISMS 1038169 2019/2020 Cell biology and technology
GENERAL MICROBIOLOGY, MICROBIAL BIOTECHNOLOGIES AND ELEMENTS OF MEDICAL MICROBIOLOGY 1034848 2019/2020 Agro-Industrial Biotechnology
Microbial vectors and applications in gene and cell therapy 1020774 2019/2020 Cell biology and technology
ENVIRONMENTAL MICROBIOLOGY 1055569 2019/2020 Ecobiology
Microbial vectors and applications in gene and cell therapy 1020774 2018/2019 Cell biology and technology
GENERAL MICROBIOLOGY, MICROBIAL BIOTECHNOLOGIES AND ELEMENTS OF MEDICAL MICROBIOLOGY 1034848 2018/2019 Agro-Industrial Biotechnology
MOLECULAR GENETICS OF MICROORGANISMS 1038169 2018/2019 Cell biology and technology
ENVIRONMENTAL MICROBIOLOGY 1055569 2018/2019 Ecobiology
Microbial vectors and applications in gene and cell therapy 1020774 2017/2018 Cell biology
MOLECULAR GENETICS OF MICROORGANISMS 1038169 2017/2018 Cell biology
ENVIRONMENTAL MICROBIOLOGY 1055569 2017/2018 Ecobiology
Microbial vectors and applications in gene and cell therapy 1020774 2016/2017 Cell biology
MOLECULAR GENETICS OF MICROORGANISMS 1038169 2016/2017 Cell biology
Title Journal Year
DNA methylation patterns correlate with the expression of SCNN1A, SCNN1B, and SCNN1G (Epithelial sodium channel, ENaC) genes INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 2021
DEVELOPMENT OF ArnT-MEDIATED COLISTIN RESISTANCE DITERPENE-BASED INHIBITORS 2021
Escaping the Phagocytic Oxidative Burst: The Role of SODB in the Survival of Pseudomonas aeruginosa Within Macrophages FRONTIERS IN MICROBIOLOGY 2020
Structural elucidation and antimicrobial characterization of novel diterpenoids from fabiana densa var. ramulosa ACS MEDICINAL CHEMISTRY LETTERS 2020
A novel colistin adjuvant identified by virtual screening for ArnT inhibitors JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY 2020
Ent-beyerane diterpenes as a key platform for the development of arnt-mediated colistin resistance inhibitors JOURNAL OF ORGANIC CHEMISTRY 2020
Anti-inflammatory potential of cow, donkey and goat milk extracellular vesicles as revealed by metabolomic profile NUTRIENTS 2020
Biofilm Production by Carbapenem-Resistant Klebsiella pneumoniae Significantly Increases the Risk of Death in Oncological Patients FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY 2020
Nanometric ion pair complexes of tobramycin forming microparticles for the treatment of Pseudomonas aeruginosa infections in cystic fibrosis INTERNATIONAL JOURNAL OF PHARMACEUTICS 2019
MiR-146a is over-expressed and controls IL-6 production in cystic fibrosis macrophages SCIENTIFIC REPORTS 2019
Staphylococcus aureus and the cutaneous microbiota biofilms in the pathogenesis of atopic dermatitis MICROORGANISMS 2019
A novel approach based on low-field NMR for the detection of the pathological components of sputum in cystic fibrosis patients MAGNETIC RESONANCE IN MEDICINE 2018
Assembly and functional analysis of an S/MAR based episome with the cystic fibrosis transmembrane conductance regulator gene INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 2018
Environmental Burkholderia cenocepacia Strain Enhances Fitness by Serial Passages during Long-Term Chronic Airways Infection in Mice INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 2017
Positively charged biopolymeric nanoparticles for the inhibition of Pseudomonas aeruginosa biofilms JOURNAL OF NANOPARTICLE RESEARCH 2016
Development of an in vitro assay, based on the biofilm ring test®, for rapid profiling of biofilm-growing bacteria FRONTIERS IN MICROBIOLOGY 2016
Campylobacter jejuni fatal sepsis in a patient with non-Hodgkin’s lymphoma: Case report and literature review of a difficult diagnosis INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 2016
Improved stability and efficacy of chitosan/pDNA complexes for gene delivery BIOTECHNOLOGY LETTERS 2015
Role of cytokine profile in the differential diagnosis between acute lung rejection and pulmonary infections after lung transplantation EUROPEAN JOURNAL OF CARDIO-THORACIC SURGERY 2015
Expression of ENaC subunits as an investigation tool of the interaction between CFTR and ENaC and therapeutic approaches by epigenetic manipulation and activity reduction. 2015
Department
BIOLOGIA E BIOTECNOLOGIE "CHARLES DARWIN"
SSD

BIO/19