Catalogo dei Corsi di studio

Students acquire the knowledge and thinking skills necessary to understand biological problems in a evolutionary perspective. The course will provide students with understanding of the basic molecular mechanisms that operate in living cells, with a focus on the flow of genetic information.

Programma

1. Definition of life. Darwinian evolution: variation, heritability and fitness. Origin of life: prebiotic chemistry, RNA world. Gene-centered view of evolution: replicators and vehicles. From molecules from the first cells. From single cells to multicellular organisms. The tree of life. The major transitions in evolution: mutualistic symbiosis and complexity
2. Proteins: structure and functions. Enzymes and biological reactions.
3. Bio-membranes: Structural Organization and Functions. Principles of membrane transport: active/passive transport, carrier proteins, ion channels, electrical properties of membranes.
4. Biological order and energy. Energy for cellular activities. Production of ATP. Structure and function of mitochondria. Glycolysis, Krebs cycle, electron transport chain. The mitochondrial ATP synthase.
5. RNA and DNA structures. DNA replication and repair. The cell nucleus: chromatin structure, epigenetic modifications.
6. Transcription and translation. RNA transcription in prokaryotes. RNA transcription and processing in eukaryotes: mRNA, tRNA, rRNA. The genetic code. Protein synthesis: initiation, elongation and termination .
7. Regulation of gene expression. Control of transcription in prokaryotes: bacterial operons. Control of transcription in eukaryotes. Post-transcriptional and translational regulation. Non-coding RNAs, microRNAs.
8. The genome organization in prokaryotic and eukaryotic organisms. Mobile elements. Genome evolution.
9. Endomembrane system: endoplasmic reticulum, Golgi. Protein sorting and glycosylation. Lysosomes. Phagocytosis and Endocytosis.
10. Principles of cell signaling: G Protein–Coupled Receptors. Effectors and second messengers. Receptor Tyrosine Kinases, MAP Kinase Pathways.
11. Eukaryotic cell cycle. Phases of cell cycle. Cyclin-dependent protein kinases (CdKs). Cell cycle checkpoints. S phase. Mitosis. Cytokinesis. Apoptosis.
12. Genetics of cancer. The hallmarks of cancer. Oncogenes and tumor suppressor genes. Somatic evolution in cancer.

Testi adottati

Alberts et al,Essential cell Biology, Garland Science.

Modalità di valutazione

Durante la prova orale agli studenti è richiesto di rispondere a quesiti attinenti gli argomenti del corso fornendo risposte dettagliate con proprietà di linguaggio.