PAOLA DEL PORTO

Curriculum vitae
Paola Del Porto

Name: Paola Del Porto
Address: Dept. of Biology and Biotechnology “C. Darwin” “Sapienza” University, Via dei Sardi 70, 00185, Rome, Italy

Education
1993 PhD thesis in Experimental Medicine at “Sapienza” University, Rome, Italy
1988 Abilitazione alla professione di Biologo
1987 Biological Science degree with honor

Employment
2020- Associate Professor MED04 at the Department Biology and Biotechnology "C. Darwin" “Sapienza” University, Rome, Italy
1996-2020 Researcher MED04 at the Department Biology and Biotechnology "C. Darwin" “Sapienza” University, Rome, Italy
1995-96 CEE fellow Necker Institut INSERM U373 Paris, France
1994-95 EMBO fellow at Necker Institut INSERM U373 Paris, France
1993-94 AIRC fellow, at the Department of Cellular and Developmental Biology “Sapienza” University, Rome Italy
1991-92 Fondation pour la Recherche Medicale fellow, laboratoire d' hemato-immunologie INSERM U333 Institut Gustave-Roussy, Villejuif, France
1989-91 Cenci Bolognetti fellow, Laboratoire d' hemato-immunologie, INSERM U333, Institut Gustave-Roussy, Villejuif, France
1988-89 PhD student of "Dottorato di Ricerca in Medicina Sperimentale “Sapienza” University Rome, Italy
1987-88 Post-graduate training at the Dept. of Cellular and Developmental Biology “Sapienza” University, Rome, Italy

Foreign languages: English and French

Editorial activity, Referee for: Journal of Molecular Medicine, Journal of Hepatology, Journal of Medical Virology, PlosONE, Journal of Cystic Fibrosis, Biochimica et Biophysica Acta, Frontiers in Bioscience-Landmark, Cells, Viruses, International Immunopharmacology.

Teaching activity:
2001-2004 Teaching “Cellular Pathology” , Biological Science Degree “Sapienza”
2004-2012 Teaching “Immunopathology” (3CFU), Master Degree “Biologia Applicata alla Ricerca Biomedica” Sapienza.
2005-2009 Teaching “Cellular Pathology” (3CFU), Master Degree in “Biologia Cellulare Applicata” Sapienza
2012- Teaching “Immunopathology” (3CFU), Master Degree in “Genetica e Biologia Molecolare” Sapienza.
2012-2020 Teaching “Molecular and Applied Immunology” (3CFU), Master Degree in “Biologia e Tecnologie Cellulari” Sapienza
2020- Teaching Molecular and Applied Immunology (6CFU) Master Degree in “Biologia e Tecnologie Cellulari” Sapienza

Publications
1. Garbuglia AR, S. Pauciullo, V. Zulian V, P. Del Porto. Update on hepatitis C vaccine: results and challenges. 2024. Viruses. 2024 Aug 21;16(8):1337. doi: 10.3390/v16081337.
2. Occhicone A, F. Michelotti, P. Rosa, D. Chiappetta, T. Pileri, P. Del Porto, N. Danz, P. Munzert, G. Pignataro, A. Sinibaldi. Enhanced fluorescence detection of miRNA by means of Bloch surface wave-based biochips. 2023. Analyst. 148(18):4429-4437. doi: 10.1039/d3an00804e
3. Cavinato L, F.R Luly, V. Pastore, D. Chiappetta, G. Sangiorgi, E. Ferrara, P. Baiocchi, G. Mandarello, G. Cimino, P. Del Porto, F. Ascenzioni. Elexacaftor-Tezacaftor-Ivacaftor corrects monocyte microbicidal deficiency in cystic fibrosis. 2022. Eur Respir J. 1:2200725. doi: 10.1183/13993003.00725-2022.
4. Blaconà G, R. Raso , S. Castellani, S. Pierandrei, P. Del Porto, G. Ferraguti, F. Ascenzioni, M. Conese, Lucarelli M. Downregulation of epithelial sodium channel (ENaC) activity in cystic fibrosis cells by epigenetic targeting. 2022. Cell Mol Life Sci. 79(5):257. doi: 10.1007/s00018-022-04190-9.
5. Garbuglia AR, C. Minosse, P. Del Porto. mRNA- and Adenovirus-Based Vaccines against SARS-CoV-2 in HIV-Positive People. 2022. Viruses. 14(4):748. doi: 10.3390/v14040748.
6. Lapa D, P. Del Porto, C. Minosse, G. D'Offizi, A. Antinori, M. R. Capobianchi, U. Visco-Comandini, F. McPhee, A.R. Garbuglia, M. Zaccarelli M. 2021. Clinical Relevance of Torque Teno Virus (TTV) in HIV/HCV Coinfected and HCV Monoinfected Patients Treated with Direct-Acting Antiviral Therapy. J Clin Med. 10(10):2092. doi: 10.3390/jcm10102092.
7. Occhicone A, P. Del Porto, N. Danz, P. Munzert, A. Sinibaldi, F. Michelotti. 2021. Enhanced fluorescence detection of Interleukin 10 by Means of 1D Photonic Crystals. Crystals. 11: 1517. https://doi.org/10.3390/cryst11121517
8. Pierandrei S, G. Truglio, F. Ceci, P. Del Porto, Bruno SM, Castellani S, Conese M, Ascenzioni F, Lucarelli M. 2021. DNA Methylation Patterns Correlate with the Expression of SCNN1A, SCNN1B, and SCNN1G (Epithelial Sodium Channel, ENaC) Genes. Int J Mol Sci. 22(7):3754. doi: 10.3390/ijms22073754.
9. Sias C, V. Guarrasi, C. Minosse, D. Lapa, F.D. Nonno, M. R. Capobianchi, A. R. Garbuglia, P. Del Porto, P. Paci. 2020 Human Papillomavirus Infections in Cervical Samples From HIV-Positive Women: Evaluation of the Presence of the Nonavalent HPV Genotypes and Genetic Diversity. Front Microbiol. 11:603657. doi: 10.3389/fmicb.2020.603657.
10. Cavinato L., E. Genise E, F. R. Luly FR, E. G. Di Domenico, P. Del Porto, F. Ascenzioni. 2020. Escaping the Phagocytic Oxidative Burst: The Role of SODB in the Survival of Pseudomonas aeruginosa Within Macrophages. Front Microbiol. 11:326. doi: 10.3389/fmicb.2020.00326.
11. Garbuglia A.R., D. Lapa, C. Sias, M. R. Capobianchi, P. Del Porto. 2020. The Use of Both Therapeutic and Prophylactic Vaccines in the Therapy of Papillomavirus Disease. Front Immunol. 11:188. doi: 10.3389/fimmu.2020.00188
12. Luly F.R, M. Lévêque, V. Licursi, G. Cimino, C. Martin-Chouly, N. Théret N, R. Negri, L. Cavinato, F. Ascenzioni, P. Del Porto. 2019. MiR-146a is over-expressed and controls IL-6 production in cystic fibrosis macrophages. Sci Rep. 9(1):16259. doi: 10.1038/s41598-019-52770-w.
13. Lapa D, A.R. Garbuglia, M.R. Capobianchi, P. Del Porto. 2019. Hepatitis C Virus Genetic Variability, Human Immune Response, and Genome Polymorphisms: Which Is the Interplay? Cells 8(4). pii: E305.doi:10.3390/cells8040305
14. Dragotto J, S. Canterini, P. Del Porto, A. Bevilacqua, MT Fiorenza. 2019. The interplay between TGF-β-stimulated TSC22 domain family proteins regulates cell-cycle dynamics in medulloblastoma cells. J Cell Physiol. 234(10):18349-18360. doi: 10.1002/jcp.28468.
15. De Rocco D, B. Pompili, S. Castellani, E. Morini, L. Cavinato, G. Cimino, M.A. Mariggiò, S.Guarnieri, M. Conese, P. Del Porto, F. Ascenzioni. 2018. Assembly and Functional Analysis of an S/MAR Based Episome with the Cystic Fibrosis Transmembrane Conductance Regulator Gene. Int J Mol Sci. pii: E1220. doi: 10.3390/ijms19041220.
16. Totani L, R. Plebani, A. Piccoli, S. Di Silvestre , P. Lanuti, A. Recchiuti, E. Cianci E, G. Dell'Elba, S. Sacchetti, S. Guarnieri, M.A. Mariggiò, V.C. Mari, M. Anile, F. Venuta, P. Del Porto, P. Moretti, M. Prioletta, F. Mucilli, M. Marchisio, A. Pandolfi, V. Evangelista, M. Romano. 2017. Mechanisms of endothelial cell dysfunction in cystic fibrosis. Biochim Biophys Acta Mol Basis Dis. 1863(12):3243-3253. doi: 10.1016/j.bbadis.2017.08.011.
17. Plebani R, R. Tripaldi, P. Lanuti, A. Recchiuti, S. Patruno, S. Di Silvestre, P. Simeone, M. Anile, F. Venuta, M. Prioletta, F. Mucilli, P. Del Porto, M. Marchisio, A. Pandolfi, M. Romano. 2017. Establishment and long-term culture of human cystic fibrosis endothelial cells. Lab Invest. 97(11):1375-1384. doi: 10.1038/labinvest.2017.74.
18. Gilardini Montani MS, M. Granato, C. Santoni, P. Del Porto, N. Merendino, G. D'Orazi, A. Faggioni, M. Cirone 2017. Histone deacetylase inhibitors VPA and TSA induce apoptosis and autophagy in pancreatic cancer cells. Cell Oncol (Dordr). 40(2):167-180. doi: 10.1007/s13402-017-0314-z.
19. Lévêque M, S. Le Trionnaire, P. Del Porto, C. Martin-Chouly. 2017. The impact of impaired macrophage functions in cystic fibrosis disease progression. J Cyst Fibros. 16(4):443-453. doi: 10.1016/j.jcf.2016.10.011.
20. Costabile G, I. d'Angelo I, R. d'Emmanuele di Villa Bianca, E. Mitidieri, B. Pompili, P. Del Porto, L. Leoni, P. Visca, A. Miro, F. Quaglia, F. Imperi, R. Sorrentino, F. Ungaro. 2016. Development of inhalable hyaluronan/mannitol composite dry powders for flucytosine repositioning in local therapy of lung infections. J Control Release. 238:80-91. doi: 10.1016/j.jconrel.2016.07.029.
21. Patella M, M. Anile, P. Del Porto, D. Diso, Y. Pecoraro, I. Onorati, S. Mantovani, T. De Giacomo, F. Ascenzioni, E. A. Rendina, F. Venuta. 2015. Role of cytokine profile in the differential diagnosis between acute lung rejection and pulmonary infections after lung transplantation Eur J Cardiothorac Surg. 47(6):1031-6. doi: 10.1093/ejcts/ezu395.
22. Di Domenico EG, E. Romano, P. Del Porto, F. Ascenzioni. 2014. Multifunctional role of the ATM/Tel1 kinase in genome stability: from the DNA damage response to telomeres maintenance. BioMed Research International 2014:787404.doi: 10.1155/2014/787404
23. Cifani N, B. Pompili, M. Anile, M. Patella, D. Diso, F. Venuta, G. Cimino, S. Quattrucci, E. G. Di Domenico , F. Ascenzioni F, P. Del Porto. 2013. Reactive-oxygen-species-mediated P. aeruginosa killing is functional in human cystic fibrosis macrophages. PLoS One. 8:e71717. doi: 10.1371/journal.pone.0071717.
24. Spada E, P. Amoroso, G. Taliani, O. Zuccaro, P. Chiriacò, P. Maio, G. Maio, M.L. Esposito, C. Mariano, R. Rinaldi, P. Bellissima, M.E. Tosti, P. Del Porto, R. Francavilla, V. Mellace, A.R. Garbuglia, A. Folgori, A. Mele; Acute Hepatitis Italian Study Group. 2013. Role of IL28B gene polymorphism and cell-mediated immunity in spontaneous resolution of acute hepatitis C. Clin Infect Dis. 57:803-11. doi: 10.1093/cid/cit402.
25. Camperio C, S. Caristi, G. Fanelli, M. Soligo, P. Del Porto, E. Piccolella. 2012. Forkhead Transcription Factor FOXP3 Upregulates CD25 Expression through Cooperation with RelA/NF-κB. PLoS One. 7(10):e48303. doi: 10.1371/journal.pone.0048303.
26. Franceschini D, P. Del Porto, S. Piconese, E. Trella, D. Accapezzato, M. Paroli, S. Morrone, E. Piccolella, E. Spada, A. Mele, J. Sidney, A. Sette, V. Barnaba. 2012. Polyfunctional type-1, -2, and -17 CD8⁺ T cell responses to apoptotic self-antigens correlate with the chronic evolution of hepatitis C virus infection. PLoS Pathog. 8(6):e1002759. doi: 10.1371/journal.ppat.1002759.
27. Del Porto P, N. Cifani, S. Guarnieri, E.G. Di Domenico, M.A. Mariggiò, F. Spadaro, S. Guglietta, M. Anile, F. Venuta, S. Quattrucci, F. Ascenzioni. 2011. Dysfunctional CFTR alters the bactericidal activity of human macrophages against Pseudomonas aeruginosa. PLoS One. 2011;6(5):e19970. doi: 10.1371/journal.pone.0019970.
28. D'Angelillo A, E. De Luna, S. Romano, R. Bisogni, W. Buffolano, N. Gargano, P. Del Porto, L. Del Vecchio, E. Petersen, M. F. Romano. 2011. Toxoplasma gondii Dense Granule Antigen 1 stimulates apoptosis of monocytes through autocrine TGF-beta signaling. Apoptosis. 16: 551-562. doi: 10.1007/s10495-011-0586-0.
29. Soligo M, C. Camperio, S. Caristi, C. Scottà, P. Del Porto, A. Costanzo, P-Y Mantel, CB. Schmidt-Weber, E. Piccolella. 2011. CD28 costimulation regulates FOXP3 in a RelA/NF-kB-dependent mechanism. Eur J Immunol. 41: 503-513. doi: 10.1002/eji.201040712.
30. Rosenberg C, S. De Craeye, E. Jongert, N. Gargano, E. Beghetto, P. Del Porto, T. Vorup-Jensen, E. Petersen. 2009. Induction of partial protection against infection with Toxoplasma gondii genotype II by DNA vaccination with recombinant chimeric tachyzoite antigens. Vaccine. 27:2489-98. doi: 10.1016/j.vaccine.2009.02.058.
31. Guglietta S, A.R. Garbuglia, L. Salichos, L. Ruggeri, A. Folgori, M.P. Perrone, C. Camperio, V. Mellace, G. Maio, P. Maio, M.R. Capobianchi, E. Spada, N. Gargano, C. Scottà, E. Piccolella, P. Del Porto. 2009. Impact of viral selected mutations on T cell mediated immunity in chronically evolving and self limiting acute HCV infection. Virology.386:398-406. doi: 10.1016/j.virol.2009.01.020.
32. Beghetto E, F.D. Paolis, A. Spadoni A, P. Del Porto, W. Buffolano, N. Gargano. 2008. Molecular dissection of the human B cell response against cytomegalovirus infection by lambda display. J Virol Methods.151:7-14. doi: 10.1016/j.jviromet.2008.04.005.
33. Scottà C, AR. Garbuglia, L. Ruggeri, E. Spada, L. Laurenti, MP. Perrone, G. Girelli, A.Mele, MR. Capobianchi, A. Folgori, A. Nicosia, P. Del Porto, E. Piccolella, Acute Hepatitis C Italian Study Group. 2008. Influence of specific CD4+ T cells and antibodies on evolution of Hypervariable Region 1 during acute HCV infection. J. Hepatol. 48:216-228. doi: 10.1016/j.jhep.2007.09.011.
34. Guglietta S, E. Beghetto, A. Spadoni, W. Buffolano, P. Del Porto, N. Gargano. 2007. Age dependent impairment of functional helper T cell responses to immunodominant epitopes of Toxoplasma gondii antigens in congenitally infected individuals. Microbes Infect. 9: 127-133.
35. Folgori A, E. Spada, M. Pezzanera, L. Ruggeri, A. Mele, AR Garbuglia, MP. Perrone, P. Del Porto, E. Piccolella , R. Cortese, A. Nicosia, A. Vitelli, Acute Hepatitis C Italian Study Group. 2006. Early impairment of hepatitis C virus specific T cell proliferation during acute infection leads to failure of viral clearance. Gut. 55:1012-9
36. Guglietta S, A.R. Garbuglia, V. Pacciani, C. Scotta, M.P. Perrone, L. Laurenti, E. Spada, A. Mele, M.R. Capobianchi, G. Taliani, A. Folgori, A. Vitelli, L. Ruggeri, A. Nicosia, E. Piccolella, P. Del Porto. 2005. Positive selection of cytotoxic T lymphocyte escape variants during acute hepatitis C virus infection. Eur J Immunol. 35:2627-2637.
37. Beghetto E, H.V. Nielsen, P. Del Porto, W. Buffolano, S. Guglietta, F. Felici, E. Petersen, N. Gargano. 2005. A combination of antigenic regions of Toxoplasma gondii microneme proteins induces protective immunity against oral infection with parasite cysts. J Infect Dis. 191:637-645.
38. Spada E, A. Mele, A. Berton, L. Ruggeri, L. Ferrigno, A.R. Garbuglia, M.P. Perrone, G. Girelli, P. Del Porto, E. Piccolella, M.U. Mondelli, P. Amoroso, R. Cortese, A. Nicosia, A. Vitelli, A. Folgori. 2004. Multispecific T cell response and negative HCV RNA tests during acute HCV infection are early prognostic factors of spontaneous clearance. Gut. 53 :1673-1681.
39. Del Porto P, M. Di Cristina, W. Buffolano, E. Beghetto, A. Spadoni, S. Guglietta, E. Piccolella, F. Felici, N. Gargano. 2004. The Toxoplasma gondii bradyzoite antigens BAG1 and MAG1 induce early humoral and cell-mediated immune response upon human infection. Microbes Infect, 6: 164-71.
40. Frasca L, C. Scottà, P. Del Porto, A. Nicosia, C. Pasquazzi, I. Versace, A. M. Masci, L. Racioppi, E. Piccolella. 2003. Antibody-selected mimics of hepatitis C virus hypervariable region 1 activate both primary and memory Th lymphocytes. Hepatology. 38: 653-63.
41. Del Porto P., G. Puntoriero, C. Scottà, A. Nicosia, E. Piccolella. 2000. High prevalence of hypervariable region 1-specific and -cross-reactive CD4+ T cells in HCV-infected individuals responsive to IFN-a treatment. Virology. 269: 313-324.
42. Carbonari M, T. Tedesco, P. Del Porto, R. Paganelli, M. Fiorilli. 2000. Human T cells with a type-2 cytokine profile are resistant to apoptosis induced by primary activation: consequences for immunopathogenesis. Clin Exp Immunol. 120: 454-62
43. Frasca L, P. Del Porto, L. Tuosto, B. Marinari, C. Scottà, M. Carbonari, A. Nicosia, E. Piccolella. 1999. Hypervariable Region 1 variants act as TCR antagonist for Hepatitis C virus-specific CD4+ T cells. J. Immunol. 1999, 163: 650-658.
44. Scala E, M. Carbonari, P. Del Porto, M. Cibati, T. Tedesco, A. M. Mazzone, R. Paganelli, M. Fiorilli. Lymphocyte Activation Gene-3 (Lag-3) expression and IFN-gamma production are variably coregulated in different human T lymphocyte subpopulations. 1998. J. Immunol. 161: 489-493.
45. Del Porto P, L. Bruno, M.G Mattei, H. von Boehmer, C. Saint Ruf. 1995. Cloning and comparative analysis of the human preT a gene. Proc. Natl. Acad. Sci. USA 92: 12105-12109.
46. Del Porto P, L. Tuosto, M.T. Fiorillo, M. D'Amato, E. Piccolella, R. Sorrentino. 1994. Identification of a novel HLA-B27 subtype by restriction analysis of a cytotoxic / T cell clone. J. Immunol. 153: 3093-3099.
47. Mami-Chouaib F, P. Del Porto, D. Delorme, T. Hercend. 1991. Further evidence for / TCR mediated TCT.1/CD48 recognition. J. Immunol. 147: 2864-2867.
48. Del Porto P, F. Mami-Chouaib, J. M. Bruneau, S. Jitsukawa, J. Dumas, M. Harnois, T. Hercend. 1991. TCT.1, a target molecule for / T cells is encoded by an Ig superfamily gene (Blast-1) located in the CD1 region of human chromosome 1. J. Exp. Med. 173: 1339-1344.
49. Mami-Chouaib F, C. Miossec, P. Del Porto, C. Flament, F. Triebel, T. Hercend. 1990. TCT.1, a novel target molecule for human non-major histocompatibility complex-restricted T lymphocytes. J. Exp. Med. 172: 1071-1076.
50. Del Gallo F, G. Lombardi, E. Piccolella, M. S. Gilardini Montani, P. Del Porto, O. Pugliese, G. Antonelli, V. Colizzi. 1990. Increased autoreactive T cell frequency in tuberculous patients. Int. Arch. Allergy Appl. Immunol. 91: 36-42.
51. Vismara D, M. Filippone Mezzopreti, M. S. Gilardini, P. Del Porto, G. Lombardi, E. Piccolella, G. Damiani, R. Rappuoli, V. Colizzi. 1990. Identification of 35- kilodalton Mycobacterium tuberculosis protein containing B- and T-cell epitopes. Infect. Immun. 58: 245-251.
52. Borboni P, G. Di Cola, G. Sesti, M. A. Marini, P. Del Porto, M. S. Gilardini Montani, R. Lauro, R. De Pirro. 1989. B- Endorphin receptors on cultured and freshly isolated lymphocytes from normal subjects. Biochem. Biophys. Res. Comm. 163: 642-648.
53. Gilardini Montani MS, F. Del Gallo, G. Lombardi, P. Del Porto, E. Piccolella, F. Arienzo, V. Colizzi. 1989. Limiting dilution analysis of T cell unresponsiveness to mycobacteria on advanced disseminated tuberculosis. Med. Microbiol. Immunol. 172: 235-244.
54. Piccolella E., G. Lombardi, M. Gobbi, M. S. Gilardini, P. Del Porto, A. Dolei, D. Fioravanti, S. Cochi, E. Mannella, V. Colizzi. 1988. Induction and regulation of human natural killer cytotoxicity by microbial antigens and recombinant IL-2. Folia Biologica. 34: 316-329.