ALESSIA MARIANO

ORCID ID:0000-0002-9569-8952
Scopus Author ID: 57216891860
SciProfiles: 1021186

SSD: BIOS-07/A

ESPERIENZE PROFESSIONALI:
2025- oggi RTDA presso il Dipartimento interdisciplinare di Benessere, Salute e Sostenibilità Ambientale
2023-2025 Assegnista di Ricerca presso il Dipartimento di Fisiologia e Farmacologia "Vittorio Erspamer"
2022-2023 Borsista presso il Dipartimento di Scienze Umane, Sociali e della Salute, Università degli studi di Cassino e del Lazio meridionale

FORMAZIONE:
2022 Dottorato di Ricerca in Biochimica presso il Dipartimento di Scienze Biochimiche "Alessandro Rossi Fanelli", Sapienza Università di Roma
2019 Laurea magistrale a ciclo unico in Chimica e Tecnologia Farmaceutiche, Sapienza Università di Roma

LINEE DI RICERCA:
My current research activity focuses on investigating the intracellular effects of nutraceutical substances on primary cell cultures. One of the most extensively studied compounds in my projects is Harpagophytum procumbens extract, commonly known as devil’s claw, traditionally used for the treatment of chronic inflammatory joint diseases such as osteoarthritis. In particular, my research has revealed the activation of several understudied intracellular pathways involved in osteoarthritis, and has highlighted an individual- dependent therapeutic response, aligning with the principles of personalized medicine.
Since 2021, my work has expanded to include the development and application of nanoparticle-based formulations, primarily utilizing N- acetylglucosamine, as an innovative drug delivery strategy. This approach aims to enhance therapeutic efficacy while minimizing the required dosage. The findings from these research lines have been published in international peer-reviewed journals and presented at scientific conferences through oral presentations.

PUBBLICAZIONI:
1. Lopreiato, M.; Mariano, A.; Cocchiola, R.; Longo, G.; Dalla Vedova, P.; Scandurra, R.; Scotto d’Abusco, A. Nanostructured TiC Layer is Highly Suitable Surface for Adhesion, Proliferation and Spreading of Cells. Condens. Matter 2020, 5, 29. Doi: 10.3390/condmat5020029.

2. Serafini, G.; Lopreiato, M.; Lollobrigida, M.; Lamazza, L.; Mazzucchi, G.; Fortunato, L.; Mariano, A.; Scotto d’Abusco, A.; Fontana, M.; De Biase, A. Platelet Rich Fibrin (PRF) and Its Related Products: Biomolecular Characterization of the Liquid Fibrinogen. J. Clin. Med. 2020, 9(4), 1099. doi: 10.3390/jcm9041099.

3. Mariano, A.; Di Sotto, A.; Leopizzi, M.; Garzoli, S.; Di Maio, V.; Gullì, M.; Dalla Vedova, P.;
Ammendola, S.; Scotto d’Abusco, A. Antiarthritic Effects of a Root Extract from Harpagophytum procumbens DC: Novel Insights into the Molecular Mechanisms and Possible Bioactive Phytochemicals. Nutrients 2020, 12, 2545. doi: 10.3390/nu12092545.

4. Lopreiato, M; Di Cristofano, S; Cocchiola, R; Mariano, A; Guerrizio, L; Scandurra, R; Mosca, L; Raimondo, D; Scotto d'Abusco, A. Biochemical and Computational Studies of the Interaction between a Glucosamine Derivative, NAPA, and the IKKα Kinase. Int J Mol Sci. 2021 Feb 6;22(4):1643. Doi: 10.3390/ijms22041643.

5. Silvestro, I; Sergi, R; Scotto d’Abusco, A; Mariano, A; Martinelli, A; Piozzi, A; Francolini, I. Chitosan scaffolds with enhanced mechanical strength and elastic response bycombination of freeze gelation, photo-crosslinking and freeze-drying. Carbohydrate Polymers. 2021 267(2021), p. 118156. doi: 10.1016/j.carbpol.2021.118156.

6. Di Giacomo, S.; Mariano, A.; Gullì, M.; Fraschetti, C.; Vitalone, A.; Filippi, A.; Mannina, L.; Scotto d’Abusco, A.; Di Sotto, A. Role of Caryophyllane Sesquiterpenes in the Entourage Effect of Felina 32 Hemp Inflorescence Phytocomplex in Triple Negative MDA-MB-468 Breast Cancer Cells. Molecules 2021, 26, 6688. doi: 10.3390/molecules26216688

7. Spano, M.; Maccelli, A.; Di Matteo, G.; Ingallina, C.; Biava, M.; Crestoni, M.E.; Bardaud, J.-X.; Giusti, A.M.; Mariano, A.; Scotto D’Abusco, A.; Sobolev, A.P.; Lasalvia, A.; Fornarini, S.; Mannina, L. Metabolomic Profiling of Fresh Goji (Lycium barbarum L.) Berries from Two Cultivars Grown in Central Italy: A Multi-Methodological Approach. Molecules 2021, 26, 5412. Doi: 10.3390/molecules26175412.

8. Mariano, A.; Bigioni, I.; Scotto d’Abusco, A.; Baseggio Conrado, A.; Maina, S.; Francioso, A.; Mosca, L.; Fontana, M. Pheomelanin Effect on UVB Radiation-Induced Oxidation/Nitration of L- Tyrosine. Int. J. Mol. Sci. 2022, 23, 267. doi: 10.3390/ijms23010267.

9. De Felice, A.C.; Di Lisio, V.; Francolini, I.; Mariano, A.; Piozzi, A.; Scotto d’Abusco, A.; Sturabotti, E.; Martinelli, A. One-Pot Preparation of Hydrophilic Polylactide Porous Scaffolds by Using Safe Solvent and Choline Taurinate Ionic Liquid. Pharmaceutics 2022, 14, 158. doi: 10.3390/pharmaceutics14010158.

10. Lollobrigida, M., Lamazza, L., Di Pietro, M., Filardo, S., Lopreiato, M., Mariano, A., Bozzuto, G., Molinari, A., Menchini, F., Piattelli, A., De Biase, A. Efficacy of Combined Mechanical and Chemical Decontamination Treatments on Smooth and Rough Titanium Surfaces and Their Effects on Osteoconduction: An Ex Vivo Study. Int J Oral Maxillofac Implants. 2022 Jan- Feb;37(1):57-66. doi:10.11607/jomi.9105

11. Mariano, A.; Bigioni, I.; Mattioli, R.; Di Sotto, A.; Leopizzi, M.; Garzoli, S.; Mariani, P.F.; Dalla Vedova, P.; Ammendola, S.; Scotto d’Abusco, A. Harpagophytum procumbens Root Extract Mediates Anti- Inflammatory Effects in Osteoarthritis Synoviocytes through CB2 Activation. Pharmaceuticals 2022, 15, 457. doi: 10.3390/ph15040457

12. Mariano, A.; Bigioni, I.; Misiti, F.; Fattorini, L.; Scotto d’Abusco, A.; Rodio, A. The Nutraceuticals as modern key to achieve erythrocyte oxidative stress fighting in Osteoarthritis. Current issue in molecular biology. 2022, 44, 3481-349. doi: 10.3390/cimb44080240.

13. Chiloeches, A.; Fernández-García, R.; Fernández-García, M.; Mariano, A.; Bigioni, I.; Scotto d'Abusco, A.; Echeverría, C.; Muñoz-Bonilla, A. PLA and PBAT based electrospun fibers functionalized with antibacterial biobased polymers. Macromolecular Bioscience.2023, 23, 2200401. doi: 10.1002/mabi.202200401.

14. Marchetti, M.; De Berardis, B.; Bigioni, I.; Mariano, A.; Superti, F.; Scotto d'Abusco, A. In Vitro Antiviral and Anti-Inflammatory Activities of N-Acetylglucosamine: Development of an Alternative and Safe Approach to Fight Viral Respiratory Infections. Int J Mol Sci. 2023 Mar 7;24(6):5129. Doi: 10.3390/ijms24065129.

15. Mariano, A.; Bigioni, I.; Ammendola, S.; Scotto d'Abusco, A. The Formulation of the N- Acetylglucosamine as Nanoparticles Increases Its Anti-Inflammatory Activities: An In Vitro Study. Bioengineering. 2023 Mar 9;10(3):343. 39. doi: 10.3390/bioengineering10030343.

16. Mallamaci, G.; Brugnoli, B.; Mariano, A.; Scotto d'Abusco, A.; Piozzi, A.; Di Lisio, V.; Sturabotti, E.; Alfano, S.; Francolini, I. Surface modification of polyester films with polyfunctional amines: Effect on bacterial biofilm formation, Surfaces and Interfaces, 2023. 39, 102924. Doi: 10.1016/j.surfin.2023.102924

17. Mariano, A.; Bigioni, I.; Marchetti, M.; Scotto d’Abusco, A.; Superti, F. Repositioned Natural Compounds and Nanoformulations: A Promising Combination to Counteract Cell Damage and Inflammation in Respiratory Viral Infections. Molecules 2023, 28, 4045. doi: 10.3390/molecules28104045.

18. Brugnoli, B.; Mariano, A.; Simonis. B.; Bombelli C.; Sennato. S., Piozzi A.; Taresco V.; Chauhan V.M.; Howdle S.M.; Scotto d'Abusco A.; Francolini I. Self-assembled chitosan-sodium usnate drug delivery nanosystems: Synthesis, characterization, stability studies, in vitro cytotoxicity and in vivo biocompatibility against 143 B cells, Carbohydrate Polymer Technologies and Applications 2023, 100373. Doi: 10.1016/j.carpta.2023.100373.

19. Brugnoli, B.; Perna, G.; Alfano, S.; Piozzi, A.; Galantini, L.; Axioti, E.; Taresco, V.; Mariano, A.; Scotto d’Abusco, A.; Vecchio Ciprioti, S.; et al. Nanostructured Poly-l-lactide and Polyglycerol Adipate Carriers for the Encapsulation of Usnic Acid: A Promising Approach for Hepatoprotection. Polymers 2024, 16, 427. Doi: 10.3390/polym16030427.

20. Ciarlantini C.; Francolini I.; Silvestro I.; Mariano A.; Scotto d'Abusco A.; Piozzi A. Design of bioactive and biomimetic scaffolds based on chitosan-alginate polyelectrolyte complexes for tissue engineering, Carbohydrate Polymers 2024, 121684. doi: 10.1016/j.carbpol.2023.121684.

21. Mariano A.; Di Cristofano S.; Raimondo D.; Scotto d'Abusco A. Split Gp41‐1 intein splicing as a model to evaluate the cellular location of the oncosuppressor Maspin in an in vitro model of osteosarcoma, Cell biochemistry and function 2024, e3987. doi: 10.1002/cbf.3987.

22. Mazzucchi, G.; Mariano, A.; Serafini, G.; Lamazza, L.; Scotto d’Abusco, A.; De Biase, A.; Lollobrigida, M. Osteoinductive Properties of Autologous Dentin: An Ex Vivo Study on Extracted Teeth. J. Funct. Biomater. 2024, 15, 162. doi: 10.3390/jfb15060162.

23. Mariano, A.; Ammendola, S.; Migliorini, A.; Leopizzi, M.; Raimondo, D.; Scotto d'Abusco, A. Intron retention in PI-PLC γ1 mRNA as a key mechanism affecting MMP expression in human primary fibroblast-like synovial cells, Cell biochemistry and function 2024, e4091. doi: 10.1002/cbf.4091.

24. Crucianelli, S. #; Mariano, A. #; Valeriani, F.; Cocomello, N.; Fontana, M. Effects of sulphur thermal water inhalations in long-COVID syndrome: Spa-centred, double-blinded, randomised case–control pilot study. Clinical Medicine. 2024, 24 (6), 100251. doi: 10.1016/j.clinme.2024.100251

25. Mariano, A.; Scotto d’Abusco, A.; Ammendola, S. A Rheological Study of Creams and Gels Containing N-Acetyl Glucosamine in Nanoparticle Form: The Advantages of a Bioengineered Strategy for Natural Anti-Inflammatory Substance Vehiculation. Appl. Sci. 2024, 14, 11752. Doi: 10.3390/app142411752.

26. Serafini G.; Mariano A.; Lollobrigida M.; Lamazza L.; Mazzucchi G.; Spigaglia P.; Barbanti F.; Scotto d’Abusco A. and De Biase A. Advanced Platelet-Rich Fibrin (A-PRF) as antibiotics delivery system: in-vitro proof-of-concept study. Materials. 2025, 18, 570. doi: 10.3390/ma18030570.

27. Mariano, A.; Bigioni, I.; Baseggio Conrado, A.; Francioso, A.; Scotto d’Abusco, A.; Fontana, M. Thiotaurine Attenuates TNF-α-Induced Inflammation in Human Chondrocytes via NF-κB Pathway Suppression and Thiol-Dependent Persulfidation. Int. J. Mol. Sci. 2025, 26, 10208. https://doi.org/10.3390/ijms262010208

28. Mariano, A.; D’Andrea, D.; Mattioli, R.; Ammendola, S.; Scotto d’Abusco, A. Molecular Basis of Intron Retention in PI-PLC γ1 mRNA from Osteoarthritis Synoviocytes. Int. J. Mol. Sci. 2025, 26, 8123. https://doi.org/10.3390/ijms26178123

29. Mariano, A.; Consalvi, V.; Marchetti, E.; Rodio, A.; Scotto d’Abusco, A.; Fattorini, L. Cell Culture in a Hyperbaric Chamber: A Research Model to Study the Effects of Hyperbarism (Hyperbaric Pressure) on Bone Cell Culture. Cells 2025, 14, 1287. https://doi.org/10.3390/cells14161287

30. Mariano, A.; Russo, R.; Scotto d’Abusco, A.; Superti, F. Astragalus membranaceus Extract Attenuates Inflammatory Cytokines and Matrix-Degrading Enzymes in Human Chondrocytes: A Novel Nutraceutical Strategy for Joint Health. Curr. Issues Mol. Biol. 2025, 47, 731. https://doi.org/10.3390/cimb47090731