Biomedical Laboratory techniques REPLICA E - REGIONE MOLISE – IRCCS NEUROMED POZZILLI

• Generalities of laboratory medicine • Biological samples preparation and preservation • Measure and biological variability: pre-analytical and analytical variability • Blood and urine sampling and preservation • Centrifugation (analytical and separative): Differential C. Density gradient C. Zonal C. Isopycnic C. Blood cells and components isolation Buffy Coat purification on Ficoll cushion Immunobeads-based isolation of cell subpopulations • Electrophoresis (horizontal and vertical E.) SDS-PAGE ElectroBlot Western Blot Cellulose acetate E. Isoelettrofocusing • Chromatography Thin layer C. Ionic exchange C. Gel filtration C. Affinity C. HPLC • Spectroscopy Generalities Absorbance and Transmittance - Lambert Beer Law Spectrophotometers Enzymatic and non-enzymatic end points measures Turbidimetry and Nephelometry (Rayleigh’s law) Fluorimetry Densitometry • Clinic Enzymology • Immunochemistry Generalities Direct and indirect agglutination Immunoprecipitation and Immunodiffusion Immunoelelectrophoresis Radio-Immuno Assay (RIA) Enzyme-Linked Immuno-Sorbent Assay (ELISA) • Flow Cytometry Fundaments • Automation in diagnostic

Pasquinelli F. Diagnostica e tecniche di laboratorio Ed. Rosini Editrice ISBN: 9788808111111