Biomedical Laboratory techniques REPLICA E - REGIONE MOLISE – IRCCS NEUROMED POZZILLI

Molecular Approaches in Parasitological Diagnostics and Research Introduction to the ADE Overview of Diagnostic Parasitology: basic concepts and comparison of different techniques Molecular methods with applications in diagnostics and research: Real-Time PCR (RT-qPCR): TaqMan and SYBR Green assays Real-Time PCR: data analysis Digital PCR Isothermal amplification methods Molecular applications in parasitological diagnostics Development of diagnostic kits Applied examples: malaria, amebiasis, Chagas disease Evaluation test and ADE conclusion Foundations of Genomics, Transcriptomics, and Gene Manipulation in Model Organisms in Parasitology Next-Generation Sequencing (NGS) Introduction to the ADE Overview of nucleic acid sequencing In-depth study of RNA-seq techniques and data analysis Gene manipulation in model organisms Introduction to gene manipulation methods in model systems Insights into the CRISPR-Cas9 technique Experimental design for proper implementation of gene-editing experiments Applications and conclusions Molecular applications in parasitological research and diagnostics Applied examples: arthropod vectors and apicomplexan parasites Evaluation test and ADE conclusion

Basic knowledge in molecular biology and parasitology

Slides provided by the teacher.

frontal lessons

attendance is compulsory

written test

frontal lessons