Biomedical Laboratory techniques REPLICA E - REGIONE MOLISE – IRCCS NEUROMED POZZILLI

The first half of year: Knowledge of accident prevention, use and maintenance of basic instrumentation: 1. Thermal balance: power, reset, calibration, weighing in grams, and decigrams centigrams, deactivation of the instrument and set aside. 2. pH meter: power, calibration using known standard solutions (pH = 4, pH = 7 and pH = 9), electrode cleaning, drying electrode set-aside of the instrument. 3. Dilutions: preparation of glassware, glassware washing, drying and storage in a stove-heated room in private. Practical solutions of dilutions of percentage by weight, percentage by volume of Normality and Molarity of solutions. 4. Practical preparation of buffer solutions at different pH: control of the same pH meter. Exercise in pipetting with automatic pipettes and traditional. 5. Equipment: filtration systems, filter papers, adaptation of the filter funnel using different ways of folding (filters, small, medium and large). 6. Use of parafilm: the closing of the equipment used (flasks and cylinders) 7. Practical realization: glass rods necessary to the laboratory activities. 8. Organization of a chemical Reagentario: book with the substances used. Recording of consumption, minimum inventory, reagents that allow a continuous supply of reagentario. Collection of safety data sheets and practical interpretation of the same. The second half year: Acquisition of autonomy in the use of basic instrumentation in other clinical settings. 1. Use of pipettes and micropipettes manual and automatic. 2. Titration and calculation of buffer solutions. 3. Centrifuge operation and maintenance. 4. Sieraggio, research and eventual removal of fibrin in the serum. 5. Staining of blood smears, manual and automatic. 6. Determination of ESR (manual and automatic). 7. Use of fume hoods and laminar flow. 8. Photometer, spectrophotometer and flame photometer. 9. Electrophoresis (agarose and cellulose acetate). 10. Maintenance and correct use of freezers and refrigerators. Recording of temperatures.

Medicina di laboratorio - Italo Antonozzi - Elio Gulletta Piccin

Examination on topics related to the program