Dietistic

General aspects of Clinical Biochemistry Sampling modality for analytical of tests, biological variability, analytical variability, reference values, sensitivity and analytical specificity, measurement errors, interference in chemical and clinical analysis. Carbohydrate metabolism: changes in glycemia, diabetes mellitus, methods for the determination of glucose in the blood and urine, curves cargo glucose, glycosylated haemoglobin, hormones involved in blood glucose homeostasis. Laboratory methodologies: Spectroscopic techniques, electrophoresis, chromatographic techniques, HPLC, immunoassays methods (RIA, EIA, ELISA), PCR. Lipids: cholesterol, triglycerides, HDL cholesterol, LDL cholesterol, apo A, apo B, basis of metabolism and alteration of lipoproteins, dosing techniques, classification of hyperlipoproteinemias and methods of determination. Pasma proteins: albumin, globulins, evaluation methods of plasma proteins, acute phase proteins (C-reactive protein, ceruplasmin, haptoglobin, complement). Enzymes: description, mechanism of action, use as diagnostic tools and as analytes, basis of dosing in clinical biochemistry, LDH, GOT, GPT, gamma-GT, AMYLASE, ALP, G-6-PDH, CHOLINESTERASE, CK

Students must have acquired the principles of general biochemistry and nutritional biochemistry

Recommended tests: Costantino Salerno: Appunti di Biochimica Clinica, Sripta Web editore. Lee W Janson and Marc E Tischler: Biochimica Clinica, Piccin ed. Didactic material and further bibliographical indications will be provided by the teacher during the lesson

The lessons will take place in frontal mode, possibly at a distance in case of need for social distancing

Compulsory attendance required by the course of study

The exam will take place with a written test and a subsequent oral test. The written test aims to evaluate the knowledge acquired at the end of the module through a test that includes open-ended questions. The oral exam aims to confirm the knowledge of the written exam. The written test has a weight of 90%, while the oral test has a weight of 10% on the final grade.

Didactic material and further bibliographical indications will be provided by the teacher during the lesson

The lessons will take place in frontal mode, possibly at a distance in case of need for social distancing. The student must achieve 67% attendance in each module of the teaching in order to take the exam