Microbiology

Course objectives

Microorganisms play a key role in the environment, in human health and in biotechnological research. The course of Microbiology aims to provide the basic principles of structure, function and evolution of microbial cells, with particular regard to bacterial cells. The knowledge and skills acquired during this course will represent a framework for the study of bioinformatics and biotechnological applications of microorganisms, and for the analysis of their impact on human health and the environment. Students who have passed the exam will know and understand (acquired knowledge) - The structural and functional diversity which is present in the microbial world; - The mechanisms responsible for the structure and functioning of bacterial cells; - The mechanisms responsible for the evolution of bacterial species; - The structure and life cycles of viruses; - The methods and strategies for the control of microbial growth. Students who have passed the exam will be able to (acquired skills): - Understand and analyse microbiological data; - Critically analyze the issues related to the evolution and diffusion of multi-resistant antibiotic bacteria; - Understand and design experimental and bioinformatics approaches for the study and exploitation of bacteria for biotechnological and environmental purposes; - Identify and develop key themes to build educational paths in microbiology.

Channel 1
GIANNI PROSSEDA Lecturers' profile

Program - Frequency - Exams

Course program
Microbial cell: study of the structure and function of the bacterial cell components. Historical introduction to microbial genetics. The cytokinesis and prokaryotic genetic material: the prokaryotic cytoskeleton; the mechanics of cell division; control of DNA replication in prokaryotes: Timing, Cleaning, Partitioning. DNA repair systems: direct, Ner, Ber, SOS, Mismatch. Biosynthesis of Peptidoglycan and related antibiotics, mechanisms of action: beta-lactam antibiotics and clavulanic acid, Vancomycin, fosfomycin, bacitracin. Gene regulation: the biosynthesis of the flagellum, the phase variation of Salmonella, the life cycle of Caulobacter crescentus, sporulation of Bacillus subtilis, Anabaena. Interaction of micro-organisms with the environment: export and secretion of proteins, signal transduction, quorum sensing, biofilms. Microbial genetic elements: the Integrons, transposons, bacteriophages. Horizontal gene transfer: transformation, transduction, Conjugation. Phage Genetics: strategies of infection and gene regulation of filamentous phages T4 and T7 Phage-Display. Bacterial Pathogenesis: common themes of microbial pathogenicity, interaction between bacteria and host.
Prerequisites
In order to profitably follow the MICROBIOLOGY course, it is highly recommended that the student has knowledge of cell biology and organic and inorganic chemistry (first-year courses).
Books
- Madigan M.T. and Martinko J.M. : Brock, Biologia dei microorganismi. Volume 1 (prof. Prosseda-Valenti ) e 2b (Valenti). optional: Snyder L. and Champness W. Molecular Genetics of Bacteria. Biblioteca Dipartimento di Biologia e Biotecnologie “C. Darwin” Glazer A.N. and , Nikaidō H. Microbial Biotechnology: fundamentals of applied microbiology. Cambridge University Press
Frequency
Attendance is recommended for at least 70% of the lessons
Exam mode
At the end of the course (usually in January), an optional written examination (in the form of multiple-choice quizzes) is proposed. By obtaining a score >18/30, the student can accept the grade obtained and proceed to the formal transcript of the same, thus finishing the course. Alternatively, or if he/she does not obtain a sufficient mark in the written examination, he/she may request to take an oral examination, the date of which may be either scheduled by the professor or proposed by the student
Lesson mode
Lessons will start on October 1st, in the Bioinformatics classroom (Psicologia I, Fisiologia Generale e Antropologia Farmacia e Medicina CU026, E01PS1L101). Microbiology lessons will be held on Monday (14:00 - 16:00) and Wednesday (16:00-19:00)
  • Lesson code1049256
  • Academic year2024/2025
  • CourseBioinformatics
  • CurriculumSingle curriculum
  • Year2nd year
  • Semester1st semester
  • SSDBIO/19
  • CFU6
  • Subject areaDiscipline biotecnologiche con finalità specifiche: biologiche e industriali