Dental School

Foundations and Tools of Molecular Biology. Techniques for nucleic acid extraction and purification. Electrophoresis and restriction enzyme digestion. Molecular cloning. Hybridization and probe-based techniques. DNA amplification: PCR and its variants. Protein analysis: protein extraction and quantification, chromatographic techniques for purification, electrophoresis, immunoblotting, and structural analysis. Introduction to gene expression. Gene expression analysis. DNA sequencing techniques: Classical Sanger sequencing, Next Generation Sequencing (NGS). The Human Genome Project and post-genomic genomics: applications in medicine and biotechnology, ethics, data management, and future perspectives.

Bruce Alberts, Rebecca Heald, Alexander Johnson, David Morgan, Martin Raff, Keith Roberts, Peter Walter. Biologia molecolare della cellula. Settima edizione.