ALESSANDRO ROSA

ESPERIENZE LAVORATIVE

2019-OGGI Professore Associato
Sapienza Università di Roma
Dip di Biologia e Biotecnologie “Charles Darwin”
P.le Aldo Moro 5, Rome, ITALY

SSD: Bio/11 - Biologia Molecolare

2011-2019 Ricercatore Universitario
Sapienza Università di Roma
Dip di Biologia e Biotecnologie “Charles Darwin”
P.le Aldo Moro 5, Rome, ITALY

SSD: Bio/11 - Biologia Molecolare

2009-2010 Direttore del Stem Cell Derivation Core - Rockefeller University
The Rockefeller University
1230 York Avenue, New York, NY USA

▪ Center for the derivation of human embryonic stem cell (hESC) and human induced pluripotent stem cell (hiPS) lines with a normal as well as diseased genetic background.

2007-2010 Postdoctoral Associate
The Rockefeller University
1230 York Avenue, New York, NY USA
Supervisor: Prof. Ali H. Brivanlou

EDUCAZIONE

2003-2007 Dottorato di ricerca in Genetica e Biologia molecolare
Sapienza Università di Roma
Dip di Genetica e Biologia Molecolare
P.le Aldo Moro 5, Rome, ITALY

1998-2003 Laurea in Scienze Biologiche – 110/110 e lode
Sapienza Università di Roma
Facoltà di Scienze MMFFNN
P.le Aldo Moro 5, Rome, ITALY

ATTIVITA’ LAVORATIVE

Premi:

Riconoscimento di eccellente insegnamento universitario. A.A. 2017-18 e 2020-21. Dalla Facoltà di Scienze MMFFNN al top 5% del corpo docente.

PNI (Premio Nazionale dell’Innovazione) 2017: menzione speciale per il miglior progetto per le pari opportunità – progetto di startup HoMoLoG.

Primo premio business plan competition StartCup Lazio 2017 - progetto di startup HoMoLoG .

Vincitore del Bioeconomy Rome 2012 Award “For the most innovative Italian intuitions of 2011 in the field of biomedical sciences”. CNCCS (Collezione Nazionale di Composti Chimici e Centro Screening).

Vincitore del RNA Society 2008 poster award nella categoria “Genetics and Development”.

Vincitore di una post-doctoral fellowship “Long term 2008 Human Frontier Science Program (HFSP)”.

Vincitore di una post-doctoral fellowship “The New York Stem Cell Foundation (NYSCF)” (2008). (Declinata in favore della “Long term 2008 Human Frontier Science Program, HFSP)”.

Vincitore del premio SIBBM 2006 (Società Italiana di Biofisica e Biologia Molecolare).

Attività editoriale:

2021-oggi Associate Editor of Stem Cell Research (specialty section of Frontiers in Cell and Developmental Biology, Frontiers in Genetics, Frontiers in Oncology and Frontiers in Bioengineering and Biotechnology).

2018–oggi Editor di Stem Cells International (Hindawi Publishing Corporation; E-ISSN:1687-9678).

2018-2021 Guest Editor per uno special issue “The RNA revolution in embryonic development and cell differentiation in health and disease”. Frontiers in Cell and Developmental Biology.

Presentazioni su invito Più di 20 convegni o seminari a carattere nazionale o internazionale.

Grants 2024-2025. AriSLA Call for projects on ALS research. Pilot Grant. “StressHUD, Investigating the Mechanisms Underlying Oxidative Stress-Induced HuD Expression in Sporadic ALS”. PI

2023-2025. PRIN PNRR 2022. Generation and characterization of a new in vitro model for GNAO1 encephalopathy based on human iPS cells and cortical organoids. PI

2023-2025. PRIN 2022. HSPB3: understanding its role in the pathophysiology of the neuromuscular system and testing its druggability for future therapeutic purposes. Co-PI (PI: Serena Carra).

2022-2025. PNRR. CN3 Spoke 3. National Center for Gene Therapy and Drugs based on RNA Technology – Neurodegeneration. PI per Sapienza Università di Roma.

2023-2026. AriSLA Call for projects on ALS research. Full Grant. Unraveling the role of SUMO2/3 as a modifier of TDP-43 solubility: a new therapeutic avenue for ALS (SUMOsolvable). FG_1/2022. Co-PI (PI: Serena Carra)

2022-2025. Muscular Dystrophy Association Research Grant 22. HSPB3: a promising candidate for the maintenance of the neuromuscular system. 952598. Co-PI (PI: Serena Carra)

2021-2022. Regione Lazio - POR FESR LAZIO 2014-2020 “PROGETTI DI GRUPPI DI RICERCA 2020”. Soluzione Integrata di Microscopia Brillouin Avanzata (SIMBA) (Integrated Solution of Advanced Brillouin Microscopy) (project number: A0375-2020-36389). PI

2021-2023. AFM Telethon. Research Grants 2021. Unraveling HSPB3 physiological functions to understand its implication in neuromuscular diseases. Co-PI (PI: Serena Carra)

2021-2024. Italian Ministry of Health. Bando Ricerca Finalizzata 2019. Dissecting the role of HCN1 in Developmental and Epileptic Encephalopathy (DEE) by exploiting patient-specific models of cerebral cortex development in vivo and in 3D cortical organoids. Co-PI (PI: Simona Lodato)

2019-2021. Istituto Pasteur Italia - Fondazione Cenci Bolognetti. Call for projects 2019. Study of the role of RNA-binding proteins in the neurodegenerative disease Amyotrophic Lateral Sclerosis. PI

2019-2021. Sapienza University “Progetto di Ateneo” 2018. Identification and characterization of RNA binding proteins and non-coding RNAs with a role in human neural specification and neurodegenerative diseases. PI

2017-2018. AriSLA Pilot Grant 2016. StressFUS - Impairment of the stress response by mutant FUS in iPSC-derived human ALS motoneurons. PI

Brevetti RM2007A000595
Title: Use of miRNA and siRNA in therapy
Inventors: Irene Bozzoni, Alessandro Fatica, Alessandro Rosa
Dep. 15-11-2007

RM2003A000335 (Foreign Ext. PCT/IT04/00038)
Title: Construction of a new vector for siRNA in vivo expression
Inventors: Irene Bozzoni, Michela Denti, Alessandro Rosa
Dep. 9-7-2003
Patent licensed to Promega Corporation for the commercialization of the product GeneClip™ U1 Hairpin Cloning Systems.

ULTERIORI INFORMAZIONI

Pubblicazioni
>70 pubblicazioni in riviste internazionali indicizzate in Scopus e/o WOS
Circa 4.000 citazioni (Scopus)
H index: 29

Pubblicazioni più rilevanti

Silvestri B, Mochi M, Mawrie D, de Turris V, Colantoni A, Borhy B, medici M, Anderson EN, Garone MG, Zammerilla CP, Simula M, Ballarino M, Pandey UB & Rosa A (2024) HuD impairs neuromuscular junctions and induces apoptosis in human iPSC and Drosophila ALS models. Nat Commun 15: 9618. Doi:10.1038/s41467-024-54004-8

Garone, M. G., Salerno, D. & Rosa, A. 2023. Digital color-coded molecular barcoding reveals dysregulation of common FUS and FMRP targets in soma and neurites of ALS mutant motoneurons. Cell Death Discov 9, 33. doi:10.1038/s41420-023-01340-1.

Garone, M.G., Birsa, N., Rosito, M., Salaris, F., Mochi, M., de Turris, V., Nair, R.R., Cunningham, T.J., Fisher, E.M.C., Morlando, M., Fratta, P., Rosa, A., 2021. ALS-related FUS mutations alter axon growth in motoneurons and affect HuD/ELAVL4 and FMRP activity. Commun Biol 4, 1025. doi:10.1038/s42003-021-02538-8

Brighi, C., Salaris, F., Soloperto, A., Cordella, F., Ghirga, S., de Turris, V., Rosito, M., Porceddu, P.F., D’Antoni, C., Reggiani, A., Rosa, A., Di Angelantonio, S., 2021. Novel fragile X syndrome 2D and 3D brain models based on human isogenic FMRP-KO iPSCs. Cell Death and Disease 12, 498. doi:10.1038/s41419-021-03776-8

Garone, M.G., Alfano, V., Salvatori, B., Braccia, C., Peruzzi, G., Colantoni, A., Bozzoni, I., Armirotti, A., Rosa, A., 2020. Proteomics analysis of FUS mutant human motoneurons reveals altered regulation of cytoskeleton and other ALS-linked proteins via 3'UTR binding. Sci Rep 10, 11827. doi:10.1038/s41598-020-68794-6

De Santis, R., Alfano, V., de Turris, V., Colantoni, A., Santini, L., Garone, M.G., Antonacci, G., Peruzzi, G., Sudria-Lopez, E., Wyler, E., Anink, J.J., Aronica, E., Landthaler, M., Pasterkamp, R.J., Bozzoni, I., Rosa, A., 2019. Mutant FUS and ELAVL4 (HuD) Aberrant Crosstalk in Amyotrophic Lateral Sclerosis. Cell Rep 27, 3818–3831.e5. doi:10.1016/j.celrep.2019.05.085

De Santis, R., Garone, M.G., Pagani, F., de Turris, V., Di Angelantonio, S., Rosa, A., 2018. Direct conversion of human pluripotent stem cells into cranial motor neurons using a piggyBac vector. Stem Cell Research 29, 189–196. doi:10.1016/j.scr.2018.04.012

De Santis, R., Santini, L., Colantoni, A., Peruzzi, G., de Turris, V., Alfano, V., Bozzoni, I., Rosa, A., 2017. FUS Mutant Human Motoneurons Display Altered Transcriptome and microRNA Pathways with Implications for ALS Pathogenesis. Stem Cell Reports 9, 1450–1462. doi:10.1016/j.stemcr.2017.09.004

Lenzi, J., Pagani, F., De Santis, R., Limatola, C., Bozzoni, I., Di Angelantonio, S., Rosa, A., 2016. Differentiation of control and ALS mutant human iPSCs into functional skeletal muscle cells, a tool for the study of neuromuscolar diseases. Stem Cell Research. doi:10.1016/j.scr.2016.06.003

Lenzi, J., De Santis, R., de Turris, V., Morlando, M., Laneve, P., Calvo, A., Caliendo, V., Chiò, A., Rosa, A., Bozzoni, I., 2015. ALS mutant FUS proteins are recruited into stress granules in induced Pluripotent Stem Cells (iPSCs) derived motoneurons. Dis Model Mech 8, 755–766. doi:10.1242/dmm.020099

Rosa, A., Brivanlou, A.H., 2011. A regulatory circuitry comprised of miR-302 and the transcription factors OCT4 and NR2F2 regulates human embryonic stem cell differentiation. EMBO J 30, 237–248. doi:10.1038/emboj.2010.319

Rosa, A., Spagnoli, F.M., Brivanlou, A.H., 2009. The miR-430/427/302 Family Controls Mesendodermal Fate Specification via Species-Specific Target Selection. Dev Cell 16, 517–527. doi:10.1016/j.devcel.2009.02.007

Rosa, A., Ballarino, M., Sorrentino, A., Sthandier, O., De Angelis, F.G., Marchioni, M., Masella, B., Guarini, A., Fatica, A., Peschle, C., Bozzoni, I., 2007. The interplay between the master transcription factor PU.1 and miR-424 regulates human monocyte/macrophage differentiation. Proc Natl Acad Sci USA 104, 19849–19854. doi:10.1073/pnas.0706963104

Fazi, F., Rosa, A., Fatica, A., Gelmetti, V., De Marchis, M.L., Nervi, C., Bozzoni, I., 2005. A Minicircuitry Comprised of MicroRNA-223 and Transcription Factors NFI-A and C/EBPα Regulates Human Granulopoiesis. Cell 123, 819–831. doi:10.1016/j.cell.2005.09.023