MEDICINA CLINICA E RIGENERATIVA IV

Obiettivi formativi

SPECIFIC OBJECTIVE At the end of the course the student must be aware of the main biological, cellular and molecular mechanisms involved in the regeneration and repair of tissues and organs and the general principles of regenerative medicine and tissue engineering, in particular skeletal, including possible applications and limits. At the end of the course the student will be able, by applying the knowledge acquired in course, to critically evaluate the role of stem cells in terms of tissue homeostasis and functional plasticity as well as their applicability in experimental models in vitro, pre-clinical and clinical trials, also in order to propose and develop tissue engineering solutions for reparative / regenerative purposes. Hematology (DEL GIUDICE): Knowledge and understanding: Knowledge of normal and pathologic hemopoiesis. Acute and chronic leukemias as models for the understanding of the development of neoplasias and of the related treatment strategies Chronic myeloid leukemia and acute promyelocytic leukemia represent illuminating examples of how you can cure leukemias without systemic chemotherapy if the underlying pathogenetic mechanism is fully understood. Knowledge of the organization and laboratory support necessary to perform hemopoietic stem cell transplantations. Knowledge of hemostasis and thrombosis concepts, in particular in relation to heart diseases. Applying knowledge and understanding: The student will be in a position to propose a research project in the field of hematology along the lines mastered during the course. He/she will for example be able to participate in a PhD program in Hematologic Sciences. Pathological Anatomy (CORSI): Knowledge and understanding: At the end of the course the student must be aware of the main biological, cellular and molecular mechanisms involved in the regeneration and repair of tissues and organs and the general principles of regenerative medicine and tissue engineering, in particular skeletal tissues, including possible applications and limits. Applying knowledge and understanding: At the end of the course the student will be able, by applying the knowledge acquired with this course, to critically evaluate the role of stem cells in terms of tissue homeostasis and functional plasticity as well as their applicability in experimental models in vitro, pre-clinical and clinical trials, also in order to propose and develop tissue engineering solutions for reparative / regenerative purposes. Immunology (PICONESE): Knowledge and understanding: Knowing the mechanisms of development of physiological or aberrant immune responses in immunopathology. Knowing the main pathogenetic mechanisms of autoimmune diseases (systemic lupus erythematosus, rheumatoid arthritis, multiple sclerosis), chronic viral infections (HBV, HCV, etc.) and tumors. Knowing the role of the various arms of adaptive immunity (B cells, CD4 T cells, CD8 and Treg) in the development of these pathologies. Knowing the main mouse models used for the study of immunity cells in the aforesaid pathologies. Applying knowledge and understanding: Apply the acquired knowledge to the analysis and interpretation of results derived from experimental research. Identify limits and critical issues in experimental models of immune-mediated diseases. Anatomy of laboratory animals (CAMPESE): Knowledge and understanding: Laboratory Animal Anatomy: short course aimed at the knowledge of the basic aspects of functional anatomy of the species most frequently used in biomedical research, that is Rodents and Lagomorphs. Description of the macroscopic and microscopic anatomy of the rat with mouse and rabbit details. Notes on hamster, guinea pig and gerbil, used to a lesser extent in biomedicine. Morphostructural features of species used as specific models in the investigation of certain diseases. Acquisition of concepts concerning the body structure of experimental animals that are essential for anyone who has to face the - ethical and biotechnological - responsibility of animal experimentation. Applying knowledge and understanding: Laboratory Animal Anatomy: At the end of the course the student must be able to identify a topic of study (where possible similar to his own scientific interests and / or to his academic profile such as, for example, internship, experimental thesis, etc.) and correlate it to the description of anatomical features significant for the development of the investigation. The student – after performing a short bibliographic research, will prepare a power point presentation in Italian or in English and will discuss his essay based on the relevant literature. Cardiology (SAVOIA) Knowledge and understanding: At the end of the course the student should know the molecular and biological mechanisms of the main cardiovascular pathologies. In particular the student will know the fundamentals of angiogenesis and heart regeneration as well as the molecular and cellular mechanisms involved in cardiac regeneration and repair included possible applicability and limitation. Applying knowledge and understanding: at the end of the course the student should be able to recognize the mechanisms of cardiac organ damage and critically evaluate the role of stem cell in cardiology in terms of tissue homeostasis and functional plasticity as well as the possible applicability in experimental and clinical models in order to elaborate tissue engineering solutions finalized to regeneration and repair. Animal models of disease (CAMPESE): Knowledge and understanding: At the end of the course the student should know: advantages and limits of the genetically modified murine models ; the main procedures for the generation, the characterization and the maintenance of murine colonies; the specific traits of the different types of genetically modified murine models; the basic knowledge concerning the italian and european laws on animal experimentation. Applying knowledge and understanding: To apply the acquired knowledge in discriminating the specific charachteristics, the advantages and the limits of the different types of genetically modified murine models, and in critically evaluating their role in the study of human pathologies; to know the constraint of the law concerning the animal experimentation Immuno-evasion models and viral oncology (SANTARELLI) Knowledge and understanding: At the end of the course the student should know: 1) the main molecular mechanisms that regulate the persistence of herpesviruses in the host; 2) herpesvirus immune evasion strategies; 3) the molecular mechanisms leading to the development of EBV- and KSHV-associated tumors. These herpesviruses are considered excellent models for studying oncogenic mechanisms; 4) the experimental approaches that have contributed to the development of the current therapeutic strategies. Applying knowledge and understanding: On the basis of the acquired knowledge, the student should be able to discuss the rationale and the experimental approaches of the scientific articles presented during the course. Also, he/she should be able to interpret the results of scientific article that will be part of the examination and, possibly, to propose an alternative experimental strategy. Endocrinology (ULISSE - BALDINI) Knowledge and understanding: For the endocrinology module (Ulisse), students should know: i) the pathophysiological basis of the main endocrine pathologies, including endocrine neoplasms, and in particular thyroid gland tumors; ii) the application of molecular biology techniques useful for the diagnosis and prognosis of endocrine neoplasms; iii) the application of biosensors in endocrinology; iv) gene therapy approaches in endocrinopathies. Applying knowledge and understanding: At the end of the Endocrinology module the students, starting from the current limits of molecular biology and biotechnologies in the field of diagnosis, therapy, prognosis and follow-up of endocrine pathologies, should become aware of the potential offered by the development of biotechnologies and their positive impact on patients’ life quality. Making judgements: The student will be able to link the pieces of knowledge acquired during the course and to associate the animal study models to the diseases presented. Establishing the appropriate experimental strategy to answer research questions in the various fields of study. Understanding and elaborating evaluations on the possibility of generating knock-out or "knock-in" animals for a gene whose mutation is causal or favorable in one of the pathologies of the disciplines that have been investigated (Cardiology, Hematology, Endocrinology, Immunology, Oncology). Judging the depth of the results and the correctness of the experimental approach. Communication skills: For this the student will be evaluated during the exam. Learning skills: The indication to the use of scientific papers published in journals with Impact Factor and reported on the PubMed website, on the topics covered by the lectures, for the preparation of the exam will produce the development of autonomous study skills and working models in the field of Biotechnologies.

Canale 1
ALESSANDRO CORSI Scheda docente

Programmi - Frequenza - Esami

Programma
Omeostasi tissutale: concetti generali (1 ora). Meccanismi di controllo della proliferazione cellulare (1 ora). Cellule staminali: proprietà biologiche, plasticità e ruolo nella rigenerazione e riparazione tissutale (3 ore). Medicina rigenerativa ed ingegneria tissutale, con particolare riferimento al tessuto scheletrico: principi, applicazioni e limiti (3 ore).
Prerequisiti
Per poter comprendere in maniera appropriata i contenuti dell’insegnamento e conseguire gli obiettivi di apprendimento previsti, all’inizio delle attività didattiche lo studente deve essere in possesso di quelle conoscenze di base acquisite e maturate durante la frequenza di corsi precedenti relative a biologia, cellulare e molecolare, biochimica, embriologia, anatomia, istologia, patologia generale ed anatomia patologica. Propedeuticità: vedi https://corsidilaurea.uniroma1.it/it/corso/2016/biotecnologie-mediche/insegnamenti/pages/86152 (Regolamento Didattico del Corso di Laurea Magistrale Interfacoltà in Biotecnologie Mediche).
Testi di riferimento
Robbins e Cotran. Le basi patologiche delle malattie. Edra Masson, IX Edizione, 2015. Lanza R, Langer R, Vacanti JP. Principles of Tissue Engineering. Academic Press, IV Edizione, 2013. o eventuali successive edizioni
Modalità insegnamento
L'insegnamento è basato essenzialmente su lezioni frontali caratterizzate da una forte componente interattiva tra il docente e gli studenti. Altre modalità didattiche possono prevedere lavori di gruppo o individuali sull’analisi di metodi e risultati di pubblicazioni su riviste scientifiche internazionali.
Frequenza
La frequenza viene verificata dal docente tramite fogli firma /appello su elenchi aggiornati forniti dalla Segreteria Didattica. L'attestazione di frequenza alle attività didattiche del Corso di insegnamento (almeno il 75% delle ore programmate) è necessaria allo studente per sostenere il relativo esame.
Modalità di esame
La valutazione consiste in una prova d’esame orale basato sull'analisi critica di un articolo scientifico selezionato dallo studente attinente al programma del corso e tiene conto di: a) capacità di discutere criticamente metodi e risultati dell'articolo scientifico selezionato; b) conoscenza degli argomenti oggetto delle domande di esame; c) adeguatezza della risposta a ciascuna delle domande in relazione alle competenze che si presuppone siano state acquisite alla fine del corso; d) logica seguita per rispondere alle domande; e) impiego di un linguaggio appropriato.
Bibliografia
Articoli scientifici relativi ad argomenti previsti nel programma saranno consigliati come strumento di studio con finalità integrative alle lezioni frontali ed ai testi adottati.
Modalità di erogazione
L'insegnamento è basato essenzialmente su lezioni frontali caratterizzate da una forte componente interattiva tra il docente e gli studenti. Altre modalità didattiche possono prevedere lavori di gruppo o individuali sull’analisi di metodi e risultati di pubblicazioni su riviste scientifiche internazionali.
  • Anno accademico2024/2025
  • CorsoBiotecnologie mediche
  • CurriculumBiomolecolare
  • Anno2º anno
  • Semestre1º semestre
  • SSDMED/08
  • CFU1
  • Ambito disciplinareMedicina di laboratorio e diagnostica