MARIA GRAZIA GIANSANTI

Maria Grazia Giansanti PhD
Institute of Molecular Biology and Pathology
Italian National Research Council (CNR-IBPM)
Department of Biology and Biotechnology
Sapienza University of Rome
Piazzale Aldo Moro, 5 - 00185 Rome - ITALY
Email mariagrazia.giansanti@uniroma1.it
Url: https://www.ibpm.cnr.it
Date and Place of Birth: May 2, 1967; Terni Italy

MATERNITY LEAVE
July 2004-January 2005

EDUCATION
1997 Residency in Applied Genetics (70/70 cum Laude), Sapienza University of Rome
1996 PhD in Genetics and Molecular Biology, Sapienza University of Rome
1991 Laurea cum Laude in Biological Sciences, Sapienza University of Rome

PROFESSIONAL EXPERIENCE
2019-ongoing Senior Researcher at CNR-IBPM
2010-ongoing Group leader at CNR-IBPM
2001- 2019 Researcher (Permanent Position) at Italian National Research Council
(CNR), Institute of Molecular Biology and Pathology (IBPM)
2000-2001 Postdoctoral fellow, Department of Genetics and Molecular Biology, Sapienza University of Rome
1998-1999 Postdoctoral Fellow, Department of Genetics and Develomental Biology, Stanford University, Margaret Fuller Laboratory

QUALIFICATIONS

August 2017 Full Professor of Genetics, National Scientific Qualification, (05/I1, BIO/18)

RESEARCH FIELD OF INTEREST

I have been studying the molecular mechanisms underlying cell division for over twenty years, with a major interest in cytokinesis. In addition, my research activities contributed to dissect the molecular pathways that control Golgi trafficking and glycosylation, revealing the link between functional disorder of the Golgi apparatus and human diseases.
Most of the research studies in my laboratory are carried out in Drosophila which offer powerful genetic tools as well as the ease of cell-based in vivo approaches.
Current research studies of my research group are focused on two main projects.

- The role of the oncoprotein Golgi Phosphoprotein 3 (GOLPH3) and membrane trafficking in cytokinesis and cell proliferation (Project funded by Fondazione AIRC). The highly conserved Golgi phosphoprotein 3 (GOLPH3) protein, characterized as a Phosphatidylinositol 4-phosphate [PI(4)P] effector at the Golgi, is overexpressed in several solid tumors including breast cancer, lung cancer and glioma. Moreover, GOLPH3 overexpression has been correlated with poor prognosis in several cancer types. By using Drosophila melanogaster and HeLa cultured cells, we have demonstrated that GOLPH3 accumulates at the cleavage furrow and is essential for cytokinesis. Additionally, we have shown that GOLPH3 interacts with molecular components of the TOR signaling pathway. The overarching aims of our research proposal are (i) to characterize the signaling pathways that require GOLPH3 protein and (ii) to understand how these pathways influence tumorigenesis.

- SNAP29/COG interplay in physiology and disease (Project funded by MUR, in collaboration with T. Vaccari and G. Callaini). Mutations affecting genes encoding subunits of the human tethering COG) complex cause monogenic forms of Congenital Disorders of Glycosylation (CDG). Similarly, loss-of-function mutations in the gene encoding the SNARE protein synaptosomal-associated protein (SNAP29) cause Cerebral Dysgenesis, Neuropathy, Ichthyosis, palmoplantar Keratoderma (CEDNIK), a rare autosomal recessive syndrome. CEDNIK shares several clinical aspects with COG-CDG, that include neurological impairment and microcephaly. The overall goal of this project is to investigate molecular mechanisms that involve the Conserved Oligomeric Golgi Complex (COG) and SNAP29 in physiology and pathology using Drosophila melanogaster as a model organism.

MAJOR RESEARCH ACHIEVEMENTS (Last 10 years)

- GOLPH3 is required for contractile ring formation during cytokinesis. This seminal study provided a significant advance in the cancer biology of GOLPH3.
Sechi S, Colotti G, Belloni G, Mattei V, Frappaolo A, Raffa GD, Fuller MT, Giansanti MG. 2014. PLoS Genet. 10(5):e1004305. (Hausmann & Co./Patek Philippe Award to
S. Sechi, for the research studies in the field of oncology, reported in this paper)

- Actomyosin constriction during cytokinesis, requires membrane trafficking from both secretory and endocytic pathways.
Giansanti M.G., Vandelìrleest TE, Jewett CE, Sechi S, Frappaolo A, Fabian L, et al. 2015. PLoS Genet. 11(11):e1005632.

-GOLPH3 binds and behaves as an effector of Rab1 in Golgi trafficking and cytokinesis.
Sechi S, Frappaolo A, Fraschin R, Capalbo L, Gottardo M, Belloni G, Glover D, Wainman A, Giansanti MG. 2017. Open Biol. Jan;7(1). pii: 160257.

-A COG7-Congenital Disorders of Glycosylation (CDG) disease model in Drosophila melanogaster which closely parallels the pathological characteristics of COG7-CDG patients, including pronounced neuromotor defects and altered N-glycome profiles with hyposialylation. Frappaolo A, Sechi S, Kumagai T, Robinson S, Fraschini R, Karimpour-Ghahnavieh A, Belloni G, Piergentili R, Tiemeyer KH, Tiemeyer M, Giansanti MG. 2017. J Cell Sci. 130, 3637-3649.

-The reciprocal dependence between Myosin II and GOLPH3 regulates centralspindlin stabilization at the invaginating plasma membrane and contractile ring structure.
Sechi S, Frappaolo A, Karimpour-Ghahnavieh A, Fraschini R, Giansanti MG. 2020. J Cell Sci. 133; jcs252965.

-The Drosophila GOLPH3 protein interacts with Fragile X mental retardation protein and Ataxin-2, suggesting a potential role in the pathophysiology of disorders of the nervous system. Sechi S, Karimpour-Ghahnavieh A, Frappaolo A, Di Francesco L, Piergentili R, Schininà E, D'Avino PP, Giansanti MG. 2021. Cells. 10(9):2336. doi: 10.3390/cells10092336.

- The first in vivo demonstration that GOLPH3 regulates organ growth by directly associating with the TOR signaling proteins. Frappaolo A, Karimpour-Ghahnavieh A, Cesare G, Fraschini R, Vaccari T, Giansanti MG. Cell Death & Disease in press.

EDITORIAL ASSIGNMENTS

October 2015-ongoing Academic Editor, Editorial Board, Plos One
January 2017-November 2018 Topic Editor, Frontiers in Genetics; Title of the Topic:Model Organisms: A Precious Resource for Understanding of the Molecular Mechanisms Underlying Human Physiology and Disease
March 2019- March 2020 Special Issue Editor, Cells MDPI; Special Issue Title: “Molecular Factors and Mechanisms Involved in Cytokinesis”
October 2019-ongoing Topic Editor, Frontiers in Cell and Developmental Biology, Title of the Topic: “Mechanisms of cytokinesis in Eukaryotes”; Co-Editors: Prof. Issei Mabuchi (University of Tokyo), Fred Chang (University of California)
February 2020-ongoing Section Editor “Cell Cycle Section”, Cells MDPI
March 2021- ongoing Special Issue Editor, Cells MDPI; Special Issue Title: “Molecular Factors and Mechanisms Involved in Cytokinesis II”
October 2021-ongoing Associate Editor, Frontiers in Genetics, “Genetics of Common and rare diseases”

SELECTED ORAL PRESENTATIONS, LECTURES AND SEMINARS

March 1999, Bellevue Washington, USA, 40th Annual Drosophila Research conference, Invited talk: “Mutations affecting cytokinesis during Drosophila male meiosis”
June 2000, Cortona, International Meeting “Regulation of Cell Division In Drosophila” Invited Talk: Relationships between the Central spindle and the Contractile Ring during Meiotic cytokinesis of Drosophila males.
December 2001, Washington DC, USA, Annual meeting American Society Cell Biology, Invited talk: “Spindle Assembly and cytokinesis in the absence of chromosomes during Drosophila male meiosis.”
October 2001, Tokyo, Japan, International Meeting “A new century of cytokinesis research”, Invited talk: “The role of central spindle during cytokinesis”
February 2011, University of Siena, Department of Evolutionary Biology, Invited Lecture: “Genetic dissection of cytokinesis in Drosophila melanogaster.”
September 2013, Totness (Exeter) UK, EMBO Workshop “Drosophila cell division cycle”, “The Drosophila orthologue of the oncoprotein GOLPH3 is required for contractile ring assembly during cytokinesis”
March 2014, San Diego California, USA, 55th Annual Drosophila Research Conference, Invited talk: “The roles of the oncoprotein GOLPH3 in contractile ring assembly and membrane trafficking during cytokinesis”
September 2014, Cambridge UK, Joint Conference of the British Society for Cell Biology and the Biochemical Society, “The Dynamic Cell” Cambridge, Invited talk: “The Drosophila orthologue of the oncoprotein GOLPH3 is required for contractile ring assembly during cytokinesis”
December 2014, Sapienza University of Rome, Department of Biology and Biotecnology, Invited Seminar: The role of membrane trafficking in cytokinesis: Drosophila melanogaster as a model system”
September 2015, Basel, Switzerland, University of Basel, Biozentrum, Invited Seminar: “Drosophila melanogaster as a model system to study cytokinesis.”
December 2016, Sapienza University of Rome, Department of Biology and Biotecnology, Meeting Biomedicine upfront in Genetics and Neurobiology; Invited Lecture: “The role of membrane trafficking in cytokinesis and human diseases”
July 2017; Cambridge UK, University of Cambridge, Department of Pathology; Invited lecture: “Using Drosophila melanogaster for dissecting the role of Golgi trafficking in cytokinesis and human diseases”
October 2017; IFOM-IEO Campus Milan; Invited lecture: “Dissecting the roles of COG7 and GOLPH3 proteins in vesicle trafficking and cytokinesis”
April 2018; Fondation de Treilles, Les Treilles, France, International meeting “Cytokinesis, final step of cell division”; Invited Seminar: “The role of GOLPH3 in cytokinesis”
August 2019; Milan, 25th International Symposium on glycoconjugates; Invited talk: “A Drosophila model for investigating the neurological defects associated with Congenital Disorder of Glycosylation Type IIe”
January 2020; Institute of Biochemistry and Cell Biology-CNR, Naples, Invited Seminar: “Drosophila melanogaster as a model system to dissect the role of membrane trafficking in cytokinesis and human diseases.”
April 2022; Brighton, UK; Conference of the Biochemical Society” From Golgi organization to Glycan function” Invited talk:” Drosophila as a model system to investigate the effects of mutations in Golgi trafficking on neurological functions and N-Glycosylation.”

FUNDED GRANTS (as Principal Investigator)

2009-2010 PI of the CNR unit, MIUR; Prin 2008 GRANT 2008ZCP2ZB, Title of the Research Unit Proposal “Genetic and molecular analysis of membrane trafficking during Drosophila cytokinesis”
2010-2013 PI, AIRC GRANT IG 2010, Id. 10775 funded by Associazione Italiana per la Ricerca sul Cancro (AIRC), “Genetic and molecular analysis of Drosophila cytokinesis”
2013-2017 PI, AIRC GRANT IG 2013, Id.14671, “The oncoprotein GOLPH3 and membrane trafficking in Drosophila cytokinesis”
2015- 2016 PI, Telethon GRANT GEP14076, “A Drosophila model for studying neurological defects associated with Congenital Disorder of Glycosylation type IIe”
2016-2018 PI, Royal Society GRANT 2016/R2 International Exchanges IE160510, “Investigating the role of the oncoprotein GOLPH3 in cytokinesis”
2018-ongoing (five years), PI, AIRC GRANT IG 2017, Id. 20779, “Golgi phosphoprotein 3 and membrane trafficking in cytokinesis and cell proliferation”
2022-ongoing (three years), PI of the CNR unit, MUR, Prin 2020, GRANT 2020CLZ5XW, Title of the Research Proposal “SNAP29/COG interplay in physiology and disease”
2023-ongoing (two years), PI, MUR, Prin 2022, GRANT 2022TLYYPS, Title of the Rresearch proposal “Dissecting the molecular mechanisms underlying Golgi phosphoprotein 3 function and regulation during cytokinesis”

REVISION OF SCIENTIFIC ARTICLES

Ad hoc-referee for International Journals including: Journal Cell Science, Current Biology, Journal of Cell Biology, Development, Developmental Biology, Open Biology, The Journal of Pathology, Plos one, Oncotarget, Scientific Reports, Chromosoma, Frontiers in Genetics, Biology Open, Cells, Frontiers in Cell and Developmental Biology, Scientific Reports, International Journal of molecular Sciences, Cancers.

AWARDS

1999, Fulbright grant for postdoctoral Fellows
1997, Fondazione Cenci Bolognetti, Prize for the best PhD thesis in the field of Pasteurian disciplines

Memberships professional organizations

2017-ongoing Italian Association of Cell Biology and Differentiation (ABCD), Membership.
2014-ongoing Genetics Society of America
2001-ongoing American Society of Cell Biology (ASCB), Membership.

ACADEMIC APPOINTMENTS

2006-2013 Adjunct Professor, Fundamental Genetics; University of Rome “La Sapienza”
2007-2008 Adjunct Professor, Medical Genetics; University of Rome “La Sapienza”

TUTORING (last ten years)

2012-ongoing Thesis Tutoring for numerous students (first and second level degree)
2012-Supervisor/Tutor of 3 PhD students (PhD program in Genetics and Molecular Biology, PhD program in Life Science)

PANELS AND EVALUATION COMMITTEES (LAST 10 years)

2013- Peer Reviewer for MIUR (Italian Ministry of Education, University and Research), in the evaluation of FIRB and PRIN National grant proposals
2013-ongoing Member of the specialist Roster, FULBRIGHT Scholar Program
2014- Peer Reviewer for University of Cambridge
2014- Peer Reviewer for BBSRC, UK in the evaluation of grant proposals
2017- External Examiner European School of Molecular Medicine, PhD in molecular Medicine
2017- Peer Reviewer, Dipartimento di Biologia e Biotecnologie, Evaluation of a PhD Thesis in Genetics and Molecular Biology
2017- European Commission Expert, Evaluation Panel of Grant proposals, call H2020-MSCA-IF-2017
2018- European Commission Expert, Evaluation Panel of Grant proposals, call H2020-MSCA-IF-2018
2019- European Commission Expert, Evaluation Panel of Grant proposals, call H2020-MSCA-IF-2019
2020- European Commission Expert, Evaluation Panel of Grant proposals, call H2020-MSCA-IF-2020
2020- 2021“Fondation pour la Recherche Médicale, Evaluation of Grant proposals, Equipes FRM 2021
2021 Peer Reviewer for BBSRC, UK in the evaluation of grant proposals
2021European Commission Expert, Evaluation Panel of Grant proposals, call H2020-MSCA-IF-2021
2022-ongoing European Commission Expert, Evaluation Panel of Grant proposals, call H2020-MSCA-IF-2022

NATIONAL AND INTERNATIONAL COLLABORATORS

Prof. Margaret Fuller, Department of Developmental Biology Stanford University, USA
Prof. Giuliano Callaini, University of Siena
Prof. Michael Tiemeyer, Complex Carbohydrate Research Center, Athens, USA
Prof. Thomas Vaccari, State University of Milan
Prof. Guy Smagghe, Ghent University, Belgium
Prof. Pier Paolo D’Avino, University of Cambridge, Cambridge UK
Dr. Andrea Musacchio, Max Plank Institute of molecular Physiology, Dortmund Germany
Dr. Marco Crescenzi, Core Facilities, ISS (Italian National Institute of Health) Roma

BIBLIOMETRY

H-index: 28 (Google Scholar), 24 (Web of Science), 24 (Scopus)
Citations: 2563 (Google Scholar); 1858 (Scopus)
Active Impact factor (Corresponding author/First/Last author), Last five years: 71,074

SELECTED PUBLICATIONS

1) Giansanti MG, Bonaccorsi S, Williams BC., Williams EV., Santolamazza C, Goldberg ML and Gatti M. (1998) Cooperative interactions between the central spindle and the contractile ring during Drosophila cytokinesis. Genes and Dev. 12: 396-410.

2) Bonaccorsi S, Giansanti MG, Gatti M. (1998) Spindle self-organization and cytokinesis during male meiosis in asterless mutants of Drosophila melanogaster. J Cell Biol. 1998 142(3):751-61.

3) Giansanti MG, Bonaccorsi S and Gatti M. (1999) The role of anillin during meiotic cytokinesis of Drosophila males. J. Cell Sci. 112: 2323-2334.

4) Bonaccorsi S, Giansanti MG and Gatti M (2000). Spindle assembly in Drosophila neuroblasts and ganglion mother cells. Nature Cell Biol., 2: 54-56.

5) Gatti M, Giansanti MG and Bonaccorsi S. (2000) The relationships between the central spindle and the contractile ring during cytokinesis in animal cells. Microsc. Res. Technique, 49:202-208.

6) Giansanti MG, Bonaccorsi S, Gatti M. 2001. The role of centrosomes and astral microtubules during asymmetric division of Drosophila neuroblasts. Development 128:1137-1145.

7) Giansanti# MG, Bonaccorsi S, Bucciarelli E, Gatti M. 2001. Drosophila male meiosis as a model system for the study of cytokinesis in animal cells.
Cell Structure and Function. 26:609-617.

8) Farkas RM, Giansanti MG, Gatti M, Fuller MT. 2003. The Drosophila Cog5 homologue is required for cytokinesis, cell elongation, and assembly of specialized Golgi architecture during spermatogenesis. Mol. Biol. Cell 14:190-200.

9) Bucciarelli E, Giansanti* MG, Bonaccorsi S., Gatti M. 2003. Spindle assembly and cytokinesis in the absence of chromosomes. J. Cell Biol. 160:993-999. Comment in J. Cell Biol. 160:989-990.

10) Giansanti MG, Farkas RM, Bonaccorsi S, Lindsley DL, Wakimoto BT, Fuller MT, Gatti M. 2004. Genetic dissection of meiotic cytokinesis in Drosophila males. Mol Biol Cell. 15:2509-2522.

11) Giansanti MG, Bonaccorsi S, Kurek R, Farkas RM, Dimitri P, Fuller MT, Gatti M. 2006. The class I PITP Giotto is required for Drosophila cytokinesis. Curr. Biol. 16: 195-201.

12) Giansanti# MG, Belloni G, Gatti M. 2007. Rab11 is required for membrane trafficking and actomyosin ring constriction in meiotic cytokinesis of Drosophila males. Mol. Biol. Cell. 18:5034-5047.

13) Bonaccorsi S, Mottier V, Giansanti MG, Bolkan BJ, Williams B, Goldberg ML, Gatti M. 2007. The Drosophila Lkb1 kinase is required for spindle formation and asymmetric neuroblast division. Development. 134:2183-2193.

14) Somma MP, Ceprani F, Bucciarelli E, Naim V, De Arcangelis V, Piergentili R, Palena A, Ciapponi L, Giansanti MG, Pellacani C, Petrucci R, Cenci G, Vernì F, Fasulo B, Goldberg ML, Di Cunto F, Gatti M. 2008. Identification of Drosophila mitotic genes by combining co-expression analysis and RNA interference. PLoS Genet. 18:4(7):e1000126.

15) Giansanti MG, Bucciarelli E, Bonaccorsi S, Gatti M. 2008. Drosophila SPD-2 is an essential centriole component required for PCM recruitment and astral-microtubule nucleation. Curr. Biol. 18:303-309.

16) Gao S, Giansanti MG, Buttrick GJ, Ramasubramanyan S, Auton A, Gatti M, Wakefield JG. 2008. Australin: a chromosomal passenger protein required specifically for Drosophila melanogaster male meiosis. J. Cell Biol. 180:521-535.

17) Szafer-Glusman E, Giansanti MG, Nishihama R, Bolival B, Pringle J, Gatti M, Fuller MT. 2008. A role for very-long-chain fatty acids in furrow ingression during cytokinesis in Drosophila spermatocytes. Curr. Biol. 18:1426-1431.

18) Robinett CC, Giansanti* MG, Gatti M, Fuller MT. 2009. TRAPPII is required for cleavage furrow ingression and localization of Rab11 in dividing male meiotic cells of Drosophila. J. Cell Sci. 122:4526-4534

19) Szafer-Glusman E, Fuller MT, Giansanti MG. 2011. Role of Survivin in cytokinesis revealed by a separation-of-function allele. Mol. Biol. Cell. 22:3779-3390.

20) Gatti M, Bucciarelli E, Lattao R, Pellacani C, Mottier-Pavie V, Giansanti MG, Somma MP, Bonaccorsi S. 2012. The relative roles of centrosomal and kinetochore-driven microtubules in Drosophila spindle formation. Exp. Cell Res. 318:1375-1380.

21) Wainman A., Giansanti MG., Goldberg M.L. and Gatti M. 2012. The Drosophila RZZ complex: roles in membrane traffic and cytokinesis. J. Cell Sci. 125:4014-4025.

22) Giansanti MG#, Sechi S, Frappaolo A, Belloni G, Piergentili R. 2012. Cytokinesis in Drosophila male meiosis. Spermatogenesis 2:185-196.

23) Giansanti MG# , Fuller MT. 2012. What Drosophila spermatocytes tell us about the mechanisms underlying cytokinesis. Cytoskeleton 69:869-891.

24) Belloni G, Sechi S, Riparbelli MG, Fuller MT, Callaini G, Giansanti MG# 2012. Mutations in Cog7 affect Golgi structure, meiotic cytokinesis and sperm development during Drosophila spermatogenesis. J. Cell Sci. 125:185-196.

25) Sechi S, Colotti G, Belloni G, Mattei V, Frappaolo A, Raffa GD, Fuller MT, Giansanti MG#. 2014. GOLPH3 is essential for contractile ring formation and Rab11 localization to the cleavage site during cytokinesis in Drosophila melanogaster. PLoS Genet. 10(5):e1004305. doi: 10.1371/journal.pgen.1004305.

26) D’Avino PP, Giansanti MG, Petronczki M. 2015. Cytokinesis in Animal Cells. Cold Spring Harb Perspect Biol. 7(4):a015834. doi: 10.1101/cshperspect.a015834.

27) Sechi S, Frappaolo A, Belloni G, Giansanti MG#. 2015. The roles of the oncoprotein GOLPH3 in contractile ring formation and membrane trafficking during cytokinesis. Biochem Soc Trans. 43:117-121

28) Sechi S, Frappaolo A, Belloni G, Colotti G, Giansanti MG#. 2015. The mutiple cellular functions of the oncoprotein Golgi phosphoprotein 3. Oncotarget 6: 3493-3506.

29) Giansanti MG#, Vandelìrleest TE, Jewett CE, Sechi S, Frappaolo A, Fabian L, Robinett CC, Brill JA, Loerke D, Fuller MT, Blankenship JT. 2015. Exocyst-dependent membrane addition is required for anaphase cell elongation and cytokinesis in Drosophila. PLoS Genet. 11(11):e1005632. doi:10.1371/journal.pgen.1005632

30) Frappaolo A, Sechi S, Belloni G., Piergentili R. and Giansanti MG#. 2016 Visualization of cleavage furrow proteins in fixed dividing spermatocytes. Methods Cell Biology. doi.org/10.1016/bs.mcb.2016.03.035

31) Sechi S, Frappaolo A, Fraschin R, Capalbo L, Gottardo M, Belloni G, Glover D, Wainman A, and Giansanti MG# 2017. Rab1 interacts with GOLPH3 and controls Golgi structure and contractile ring constriction during cytokinesis in Drosophila melanogaster. Open Biol. 2017. 7(1). pii: 160257

32) Frappaolo A., Sechi S., Kumagai T., Robinson S., Fraschini R., Karimpour Ghahnavieh A., Belloni G., Piergentili R. Tiemeyer KH., Tiemeyer M., Giansanti MG#. 2017. COG7 deficiency in Drosophila generates multifaceted developmental, behavioral, and protein glycosylation phenotypes. J. Cell Sci. 130:3637-3649

33) Frappaolo A, Sechi S, Kumagai T, Karimpour-Ghahnavieh A, Tiemeyer M, Giansanti MG#. 2018. Modeling Congenital Disorders of N-Linked Glycoprotein Glycosylation in Drosophila melanogaster. Front Genet. 9:436. doi: 10.3389/fgene.2018.00436

34) Giansanti MG#, Fraschini R. 2019. Editorial: Model Organisms: A Precious Resource for the Understanding of Molecular Mechanisms Underlying Human Physiology and Disease. Front Genet. 10:822. doi:10.3389/fgene.2019.00822

35) Sechi S, Frappaolo A, Karimpour-Ghahnavieh A, Gottardo M, Burla R, Di Francesco L, Szafer-Glusman E, Schininà E, Fuller MT, Saggio I, Riparbelli MG, Callaini G, Giansanti MG#. 2019. Drosophila Doublefault protein coordinates multiple events during male meiosis by controlling mRNA translation. Development. 146(22). pii: dev183053. doi: 10.1242/dev.183053.

36) Sechi S, Frappaolo A, Karimpour-Ghahnavieh A, Piergentili R, Giansanti MG#. 2020. Oncogenic Roles of GOLPH3 in the Physiopathology of Cancer. Int J Mol Sci. 21(3):933. doi: 10.3390/ijms21030933.

37) Sechi S, Frappaolo A, Karimpour-Ghahnavieh A, Fraschini R, Giansanti MG#. 2020. A novel coordinated function of Myosin II with GOLPH3 controls centralspindlin localization during cytokinesis in Drosophila. J Cell Sci. 133(21):jcs252965. doi: 10.1242/jcs.252965.

38) Frappaolo A, Karimpour-Ghahnavieh A, Sechi S, Giansanti MG#. 2020. The Close Relationship between the Golgi Trafficking Machinery and Protein Glycosylation. 9(12):2652. doi: 10.3390/cells9122652.

39) Mabuchi I, Giansanti MG, Chang F. 2021. Editorial: Mechanisms of Cytokinesis in Eukaryotes. Front Cell Dev Biol. Mar 19;9:668705. doi: 10.3389/fcell.2021.668705.

40) Sechi S, Karimpour-Ghahnavieh A, Frappaolo A, Di Francesco L, Piergentili R, Schininà E, D'Avino PP, Giansanti MG#. 2021. Identification of GOLPH3 Partners in Drosophila Unveils Potential Novel Roles in Tumorigenesis and Neural Disorders.
Cells. 10(9):2336. doi: 10.3390/cells10092336.

41) Frappaolo A, Piergentili R, Giansanti MG# 2022. Microtubule and Actin Cytoskeletal Dynamics in Male Meiotic Cells of Drosophila melanogaster. Cells. 11(4):695.

42) Giansanti MG, Piergentili R. 2022. Linking GOLPH3 and Extracellular Vesicles Content-a Potential New Route in Cancer Physiopathology and a Promising Therapeutic Target is in Sight? Technol Cancer Res Treat. doi:10.1177/15330338221135724.

43) Sechi S, Piergentili R, Giansanti MG# 2022. Minor Kinases with Major Roles in Cytokinesis Regulation. Cells. 11(22):3639. doi:10.3390/cells11223639.

44) Frappaolo A, Karimpour-Ghahnavieh A, Cesare G, Fraschini R, Vaccari T, Giansanti MG# GOLPH3 protein controls organ growth by interacting with TOR signaling proteins in Drosophila. Cell Death & Dis Nov 27;13(11):1003. doi: 10.1038/s41419-022-05438-9.

45) Frappaolo A, Giansanti MG. Using Drosophila melanogaster to Dissect the Roles of the mTOR Signaling Pathway in Cell Growth. Cells. 2023; 12(22):2622. doi: 10.3390/cells12222622.
(*) Joint first author; (#) Corresponding author