CARLO PRESUTTI

Corsi del I semestre

a) Corso di Laurea in Biotecnologie: insegnamento di Biologia Molecolare-Modulo RNA

Lunedi      ore 9:30/11:30

Giovedi   ore 9:30/10:30 alternato con il modulo di DNA

Aula B2 Odontoiatria Viale Regina Elena

b) Corso di Laurea in Neurobiologia: insegnamento di Neurobiologia Molecolare

Martedi-Giovedi     ore 9/11 

Aula B "G.Tecce" - Ist. Fisiologia Generale CU026

Corsi del II semestre

Ingegneria Genetica e Terapia Genica

Lunedi-Mercoledi  ore 16-18

Aula C - Ist. Fisiologia Generale CU026

NAME Carlo Presutti

INSTITUTION Dip. Biologia e Biotecnologie – Università Sapienza - Roma
POSITION TITLE Associate Professor

Personal Statement
Prof. Carlo Presutti, has been working on regulation of RNA stability since 1986 at the Department of Genetics and Molecular Biology of University of Rome “La Sapienza”. After his return to Italy in 2001, he started his independent lab, working initially on yeast mRNAs decay and translation. Since 2005 he focused his studies first on the role of microRNAs in different types of mammalian cells and more recently on the characterization of Long Non Coding RNA (lncRNA) and circular RNA (circRNA). During the last few years he started different and fruitful collaborations with many research groups in Italy and abroad. His lab has now an established skillness in the characterization of expression pattern of microRNAs and ncRNAs, especially by Next Generation Sequencing (NGS) microarray and q-PCR profiling. At the present the most important research lines of the Lab are:
-Role of non-coding RNAs in establishment and progression of melanoma
-Role of microRNAs in inflammation processes
-Non coding RNAs as biomarkers in different human pathologies
-Processing and stability of mRNAs in Central Nervous System

POSITIONS
INSTITUTION DIVISION/RESEARCH GROUP LOCATION POSITION YEAR
1) Department of Genetics and Molecular Biology Rome - Italy Post-Doc 1987-1990
2) Department of Genetics and Molecular Biology Rome – Italy Researcher 1991-1997
3) Wellcome Trust Institute Edinburgh (UK) Senior Researcher 1997-2000
4) Department of Biology and Biotechnology Rome – Italy Assoc.Professor 2001-present

Publications:

1) Presutti C., Lucioli A. and Bozzoni I. (1988) - Ribosomal protein L2 in S.cerevisiae is homologus to ribosomal protein L1 in X.laevis: isolation and characterization of the genes - J. Biol. Chem., 263, 6188-6192.

2) Lucioli A., Presutti C., Ciafrè S.A., Caffarelli E., Fragapane P. and Bozzoni I. (1988) - Gene dosage alteration of L2 ribosomal protein genes in S.cerevisiae: effects on ribosome biosynthesis - Mol. Cell. Biol., 8, 4792-4798.

3) Fragapane P., Caffarelli E., Cutruzzola F., Lucioli A., Presutti C. and Bozzoni I. -(1988)- Specific RNA binding proteins in X. laevis oocytes - J. Cell. Biochem. 12, 7-8

4) Presutti C., Caffarelli E., Ciafre'S.A., Fragapane P., Lener M., Prislei S., Santoro B., Sperandio S. and Bozzoni I. (1990) - Evolutionary comparison of the regulation of the ribosomal protein genes - Recombinant DNA Technologies in industry and agriculture: state of the art in Italy - p. 81-87

5) Della Seta F., Ciafré S.A., Marck C., Santoro B., Presutti C., Sentenac A. and Bozzoni I. (1990) - The ABF1 factor is the transcriptional activator of the L2 ribosomal protein genes in Saccharomyces cerevisiae - Mol. Cell. Biol., 10, 2437-2441.

6) Presutti C. and Santoro B. (1991) - Expression vectors and gene transfer - Ann.Ist.Super.Sanita’, 27, 105-114

7) Presutti C., Ciafrè S.A. and Bozzoni I. (1991) - The ribosomal protein L2 in S.cerevisiae controls the level of accumulation of its own mRNA - EMBO J., 10, 2215-2221.

8) Presutti C., Fragapane P., Caffarelli E. and Bozzoni I. (1991) Regulation of the expression of ribosomal protein genes in Eucaryotes - Minerva Biotech. 3, 197-203

9) Prislei S., Sperandio S., Fragapane P., Caffarelli E., Presutti C. and Bozzoni I. (1992) - The mechanisms controlling ribosomal protein L1 pre-mRNA are maintained in evolution and rely on conserved intron sequences - Nucleic Acids Res., 17, 4473-4479

10) Presutti C., Villa T. and Bozzoni I. (1993) - The primary sequence of the Schizosaccharomyces pombe protein homologous to S. cerevisiae ribosomal protein L2. Nucleic Acids Res., 21, 3900

11) Prislei S., Michienzi A., Presutti C., Fragapane P. and Bozzoni I. (1993) - Two different snoRNAs are encoded in introns of amphibian and human L1 ribosomal protein genes. - Nucleic Acids Res., 21, 5824-5830

12) Presutti C., Villa T., Hall D., Pertica C. and Bozzoni I. (1995) - Identification of the cis-elements mediating the autogenous control of ribosomal protein L2 mRNA stability in yeast. EMBO J. 14 4022-4030

13) Prislei S., Fatica A., De Gregorio E., Arese M., Fragapane P., Caffarelli E., Presutti C. and Bozzoni I. (1995) Self-cleaving motifs are found in close proximity to the sites utilized for U16 snoRNA processing. Gene 163, 221-226

14) Villa T., Ceradini F., Presutti C. and Bozzoni I. (1998) - Processing of the intron-encoded U18 small nucleolar RNA in the yeast Saccharomyces cerevisiae relies on both exo- and endonucleolytic activities. Mol. Cell. Biol. 18, 3376-3383

15) Hurt E., Sträßer K., Segref A., Schlaich N., Bailer S., Presutti C., Tollervey D. and Jansen R. (2000) - Mex67p Mediates Nuclear Export of Different Pol II Transcripts Including mRNAs for HSP70 and ASH1. J. Biol. Chem. 275, 8361-8368

16) Bousquet-Antonelli C., Presutti C. and Tollervey D. (2000) Identification of a regulated pathway for nuclear pre-mRNA turnover. Cell 102, 765-775

17) Santapaola D, Casalino M, Petrucca A, Presutti C, Zagaglia C, Berlutti F, Colonna B, Nicoletti M. (2002) Enteroinvasive Escherichia coli virulence-plasmid-carried apyrase (apy) and ospB genes are organized as a bicistronic operon and are subject to differential expression. Microbiology 148, 2519-2529

18) Galardi S, Fatica A, Bachi A, Scaloni A, Presutti C. and Bozzoni I. (2002) Purified box C/D snoRNPs are able to reproduce site-specific 2'-O-methylation of target RNA in vitro. Mol Cell Biol 22, 6663-8.

19) Lusi EA, Guarascio P, Presutti C, Villani R, Pellicelli A and Soccorsi F. (2005) One-step nested PCR for detection of 2 LTR circles in PBMCs of HIV-1 infected patients with no detectable plasma HIV RNA. J Virol Methods 125:11-13

20) Presutti C, Rosati J, Vincenti S. and Nasi S. (2007) Non Coding RNA and Brain. BMC Neuroscience 7:5-10

21) Vincenti S, De Chiara V, Bozzoni I. and Presutti C. (2007) The position of yeast snoRNA coding regions within host introns is essential for their biosynthesis and for efficient splicing of the host pre-mRNA. RNA 13: 138-150

22) Del Vescovo V, Casagrande V, Bianchi MM, Piccinni E, Frontali L, Militti C, Fardeau V, Devaux F, Di Sanza C, Presutti C, Negri R. (2008) Role of Hog1 and Yaf9 in the transcriptional response of Saccharomyces cerevisiae to caesium chloride. Physiol Genomics 33:110-120

23) Anastasiadou E, Vaeth S, Cuomo L, Boccellato F, Vincenti S, Cirone M, Presutti C, Junker S, Winberg G, Frati L, Wade PA, Faggioni A, Trivedi P. (2009) Epstein-Barr virus infection leads to partial phenotypic reversion of terminally differentiated malignant B cells. Cancer Lett. 284:165-74.

24) Rinaldi A, Vincenti S, De Vito F, Bozzoni I, Oliverio A, Presutti C, Fragapane P, Mele A. Stress induces region specific alterations in microRNAs expression in mice. (2010) Behav Brain Res. 208:265-269

25) Anastasiadou E, Boccellato F, Vincenti S, Rosato P, Bozzoni I, Frati L, Faggioni A, Presutti C. and Trivedi P. (2010) Epstein-Barr virus encoded LMP1 downregulates TCL1 oncogene through miR-29b. Oncogene. 29:1316-1328

26) Rosati J., Spallotta F., Nanni S., Grasselli A., Antonini A., Vincenti S., Presutti C., Colussi C., D'Angelo C., Biroccio A., Farsetti A., Capogrossi M.C., Illi B., Gaetano C. (2011). Smad-Interacting Protein-1 and MicroRNA 200 Family Define a Nitric Oxide-Dependent Molecular Circuitry Involved in Embryonic Stem Cell Mesendoderm Differentiation. Arteriosclerosis, Thrombosis and Vascular Biology. 31:898-907

27) Vincenti S., Brillante N., Lanza V., Bozzoni I., Presutti C., Chiani F., Etna M.P. and Negri R. (2011). HUVEC cells respond to radiation by inducing the expression of pro-angiogenic microRNAs. Radiation Research. 175:535-546,

28) Rosato P., Anastasiadou E., Garg N., Lenze D., Boccellato F., Vincenti S., Severa M., Coccia E., Bigi R., Cirone M., Ferretti E., Campese A., Hummel A. M., Frati L., Presutti C., Faggioni A. and Trivedi P.(2012). Differential regulation of miR-21 and miR-146a by Epstein-Barr virus encoded EBNA2. LEUKEMIA. 26(11):2343-52

29) Parlato S, Bruni R, Fragapane P, Salerno D, Marcantonio C, Borghi P, Tataseo P, Ciccaglione AR, Presutti C, Romagnoli G, Bozzoni I, Belardelli F, Gabriele L. (2013). IFN-α regulates Blimp-1 expression via miR-23a and miR-125b in both monocytes-derived DC and pDC. PLoS One. 2013 Aug 16;8(8):e72833. doi: 10.1371

30) Mannironi C, Camon J, De Vito F, Biundo A, De Stefano ME, Persiconi I, Bozzoni I, Fragapane P, Mele A, Presutti C. (2013) Acute stress alters amygdala microRNA miR-135a and miR-124 expression: inferences for corticosteroid dependent stress response. PLoS One. 2013 Sep 4;8(9):e73385. doi: 10.1371

31) Alteri A, De Vito F, Messina G, Pompili M, Calconi A, Visca P, Mottolese M, Presutti C, Grossi M. (2013) Cyclin D1 is a major target of miR-206 in cell differentiation and transformation. Cell Cycle. 2013 Dec 15;12(24):3781-90. doi: 10.4161

32) Tomaselli S, Galeano F, Alon S, Raho S, Galardi S, Polito VA, Presutti C, Vincenti S, Eisenberg E, Locatelli F, Gallo A.(2015) Modulation of microRNA editing, expression and processing by ADAR2 deaminase in glioblastoma. Genome Biol. 2015 Jan 13;16:5. doi: 10.1186/s13059-014-0575-z.

33) Capitano F, Camon J, Ferretti V, Licursi V, De Vito F, Rinaldi A, Vincenti S, Mannironi C, Fragapane P, Bozzoni I, Oliverio A, Negri R, Presutti C, Mele A. (2016) microRNAs Modulate Spatial Memory in the Hippocampus and in the Ventral Striatum in a Region-Specific Manner. Mol Neurobiol. 2016 Aug 26

34) Del Vecchio G., De Vito F., Saunders S.J., Risi A., Mannironi C., Bozzoni I. and Presutti C. (2016) RNA-binding protein HuR and the members of miR-200 family play an unconventional role in the regulation of c-Jun mRNA RNA Oct;22(10):1510-21

35) Mandolesi G., De Vito F., Musella A.,Gentile A., Bullitta S., Fresegna D., Sepman H., Di Sanza C., Haji N., Mori F., Buttari F., Perlas E., Ciotti M.T., Hornstein E., Bozzoni I., Presutti C. and Centonze D. “miR-142-3p is a key regulator of IL-1β-dependent synaptopathy in neuroinflammation" (2017) J. Neuroscience 37(3):546-561.

36) Capitano F, Camon J, Licursi V, Ferretti V, Maggi L, Scianni M, Del Vecchio G, Rinaldi A. Mannironi C, Limatola C, Presutti C, Mele A. (2017) MicroRNA-335-5p modulates spatial memory and hippocampal synaptic plasticity. Neurobiol Learn Mem. 2017 Dec 27;139:63-68.

37) Fragale A, Romagnoli G, Licursi V, Buoncervello M, Del Vecchio G, Giuliani C, Parlato S, Leone C, De Angelis M, Canini I, Toschi E, Belardelli F, Negri R, Capone I, Presutti C, Gabriele L. (2017) Antitumor effects of epidrug/IFNα combination driven by modulated gene signatures in both colorectal cancer and dendritic cells. Cancer Immunol Res. 2017 Jul;5 (7):604-616. doi: 10.1158/2326-6066

38) Licursi V, Cestelli Guidi M, Del Vecchio G, Mannironi C, Presutti C, Amendola R, Negri R. Leptin induction following irradiation is a conserved feature in mammalian epithelial cells and tissues. (2017) Int J Radiat Biol. 2017 Sep;93:947-957.doi:10.1080/09553002.2017.1339918. Epub 2017 Jul 10.

39) Mannironi C, Biundo A, Rajendran S, De Vito F, Saba L, Caioli S, Zona C, Ciotti T, Caristi S, Perlas E, Del Vecchio G, Bozzoni I, Rinaldi A, Mele A, Presutti C. (miR-135a Regulates Synaptic Transmission and Anxiety-Like Behavior in Amygdala. (2018) Mol Neurobiol. 2018 Apr;55(4):3301-3315. doi: 10.1007/s12035-017-0564-9.

40) Mocavini I, Pippa S, Licursi V, Paci P, Trisciuoglio D, Mannironi C, Presutti C, Negri R
JARID1B expression and its function in DNA damage repair are tightly regulated by miRNAs in breast cancer. Cancer Sci. 2019 Apr;110(4):1232-1243. doi: 10.1111/cas.13925. Epub 2019 Mar 18.

41) Gasparini S, Del Vecchio G, Gioiosa S, Flati T, Castrignano T, Legnini I, Licursi V, Ricceri L, Scattoni ML, Rinaldi A, , Mannironi C, Presutti C. Differential expression of Hippocampus circular RNAs in BTBR mouse model for Autism Spectrum Disorder. Mol.Neurobiology 2020 May; 2301-2313

41) Gasparini S, Licursi V, Presutti C, Mannironi C. The secret garden of neuronal circ-RNAs. Cells. 2020 July Vol.9

42) Mandolesi G., et al. The microRNA let-7b-5p Is Negatively Associated with Inflammation and Disease Severity in Multiple Sclerosis. Cells. 2021 Feb 5;10(2):330.
doi: 10.3390

43) Fragale, Alessandra; Stellacci, Emilia; Romagnoli, Giulia; Licursi, Valerio; Parlato, Stefania; Canini, Irene; Remedi, Giacomo; Buoncervello, Maria; Matarrese, Paola; Pedace, Lucia; Ascione, Barbara; Pizzi, Simone; Tartaglia, Marco; D'Atri, Stefania; Presutti, Carlo; Capone, Imerio; Gabriele L. International Journal of Cancer 2023 153(5), pp. 1080-1095
Reversing vemurafenib-resistance in primary melanoma cells by combined romidepsin and type I IFN treatment through blocking of tumorigenic signals and induction of immunogenic effects

44) Stabile, Francesca; Torromino, G.; Rajendran, S.; Del Vecchio, G.; Presutti, C.; Mannironi, C.; De Leonibus, E.; Mele, A.; Rinaldi, A. Molecular Neurobiology 2024 61(5), pp. 3031-3041 Short-Term Memory Deficit Associates with miR-153-3p Upregulation in the Hippocampus of Middle-Aged Mice
45) Gioiosa Silvia, Gasparini Silvia, Presutti Carlo, Rinaldi Arianna, Castrignanò Tiziana, Mannironi Cecilia. Scientific Reports 2025 - Integrated gene expression and alternative splicing analysis in human and mouse models of Rett syndrome.
46) De Santis Alessandro, De Santis Lucrezia, Rossi Francesca, Gasparini Silvia, Licursi Valerio, Amico Vito Antonio, Capone Imerio, Fragale Alessandra, D’Atri Stefania, Gabriele Lucia, Presutti Carlo. Cancer Medicine - 2025 “NSD2 and miRNAs as Key Regulators of Melanoma Response to Romidepsin and Interferon-α2b Treatment”- In Press