Notizie
CORSO Biologia Cellulare (Biotecnologie Agro-alimentari e Industriali)
Il corso inizia il 7 ottobre 2024, ore 11:00, in aula II NEC (CU032).
Gli studenti sono pregati di iscriversi alla pagina e-Learning del corso.
Le lezioni si terranno in presenza.
Le slide delle lezioni sarano disponibili nel sito eLearning del corso:
CORSO Biologia Cellulare e Istologia (Scienze Biologiche)
Il corso inizia il 7 ottobre 2024, ore 11:00, in aula II NEC (CU032).
Gli studenti sono pregati di iscriversi alla pagina e-Learning del corso.
Le lezioni si terranno in presenza.
Le slide delle lezioni sono disponibili nel sito eLearning del corso (piattaforma Moodle):
CORSO Biotecnologie Cellulari, modulo Cellule Animali (Scienze Biologiche)
Il corso inizia il 27 febbraio 2025, e si terrà i martedì e giovedì 11-13, in aula 218bis (CU026, piano 1).
Gli studenti sono pregati di iscriversi alla pagina e-Learning del corso.
Le lezioni si terranno in presenza.
Le slide delle lezioni saranno disponibili nel sito eLearning del corso (piattaforma Moodle):
Orari di ricevimento
Lunedì e mercoledì dalle 9.30 alle 10.30
Curriculum
http://bbcd.bio.uniroma1.it/bbcd/users/miranda-banos-maria-elena
CURRICULUM VITAE
February 2018
Personal information
Full name: Maria Elena Miranda Baños
Place and date of birth: Madrid, Spain, 20 July 1972
Nationality: Spanish
Current position: Assistant professor
Current address: Dipartimento di Biologia e Biotecnologie ‘Charles Darwin’,
Sapienza Università di Roma
Piazzale Aldo Moro n. 5, 00185, Roma, Italia
Tel./Fax: +39 06 4991 2637/ +39 06 4991 2351
E-mail: mariaelena.mirandabanos@uniroma1.it
Education
- 10/1990-07/1995: Undergraduate student, University of Malaga (Spain).
- 07/1995: Degree in Biological Sciences, score: 9.48 over 10.
- 07/1995: Master Degree, University of Malaga (Spain). Maximum score.
- 07/1995-01/2001: PhD student on ‘Experimental procedures in Cell and Molecular Biology’, University of Malaga (Spain).
- 05/1999-07/1999: research stage at the University of Clermont-1, Clermont-Ferrand, France.
- 05/2000-07/2000: research stage at the University Austral, Valdivia, Chile.
- 01/2001: PhD degree, University of Malaga (Spain). Maximum score.
Awards
- 09/1996: Extraordinary Award for Science Degree (Biology), University of Malaga (Spain).
- 01/1996-12/1999: PhD Fellowship from the Spanish Ministry of Education and Science.
Employment and research experience
- 07/1992-05/2001: undergraduate and graduate student, Laboratory of Animal Physiology, University of Malaga, Spain. Research field: analysis of the secretory products of the subcommissural organ.
- 05/2001-10/2002: Research Associate, Dept. of Anatomy, University of Cambridge, UK. Research field: retinal axon guidance during Xenopus laevis development.
- 10/2002-12/2008: Research Associate, Department of Medicine, University of Cambridge, Cambridge Institute for Medical Research, UK. Research field: cell biology of mutants of neuroserpin and alpha1 antitrypsin that lead to polymerisation and human disease.
- Present: permanent researcher (ricercatore) at Department of Biology and Biotechnology ‘Charles Darwin’, University of Rome ‘La Sapienza’, Italy. Research field: cell biology of mutants of neuroserpin and alpha1 antitrypsin that lead to polymerisation and human disease.
Teaching experience
- 1995-2000: teaching of practical sessions to undergraduate students, University of Malaga (Spain).
- 2002-present: supervision of PhD and undergraduate students.
- 2011-present: lecturer in cell biology.
Participation at international meetings
- 12/2003: ‘43rd Annual Meeting of The American Society for Cell Biology’, San Francisco, USA. Poster (first author).
- 06/2004: FASEB Conference on ‘Protein misfolding, amyloid and conformational disease’. Snowmass, Colorado, USA. Poster (first author).
- 06/2005: The 4th International Symposium on Serpin Structure, Function and Biology, Cairns, Australia. Poster (first author).
- 07/2008: The 5th International Symposium on Serpin Structure, Function and Biology, Leuven, Belgium. Poster (first author).
- 10/2012: Investigators meeting of the Alpha1 Foundation, Miami, USA. Oral presentation.
- 05/2013: EMBO meeting: The biology of Molecular Chaperones: from Molecules, Organelles and Cells to Misfolding Diseases, Santa Margherita di Pula, Italy. Two posters (last author and coauthor).
- 03-04/2014: The 7th International Symposium on Serpin Structure, Function and Biology, Leogang, Austria. Oral presentation.
- 03/2017: The 8th International Symposium on Serpin Structure, Function and Biology, Shanghai, China. Invited speaker.
Technical experience
- Cell biology: histological techniques for optic microscopy. Immunocytochemistry with enzymatic and fluorescent probes. Optic and confocal microscopy.
- Cell cultures: culture of adherent and non-adherent cell lines and primary cell cultures. Transient and stable cell transfection.
- Antibody techniques: production of polyclonal and monoclonal antibodies. Purification and isotyping of antibodies. Immunostaining. ELISA. Western Blot. Immunoprecipitation.
- Protein techniques: SDS and native PAGE. Electrotransference and western blot. Protein purification and immunoaffinity purification of proteins. Radioactive pulse-chase with 35S.
- Molecular Biology: DNA purification, molecular cloning, restriction analysis. Generation of constructs for overexpression of genes in eukaryotic cells. PCR.
- Bioinformatics: sequence alignment. PCR primer design.
- Xenopus laevis embryology: production, culture and staging of Xenopus embryos. Microdissections of early embryos. Expose brain preparations. Horseradish peroxidase labeling of Xenopus optic tract.
Languages
Spanish: Native speaker
English: Fluent
Italian: Fluent
Grants
- Pasteur Institute-Cenci Bolognetti Foundation, Italy. Cellular pathways involved in the toxicity of neuroserpin polymers that cause dementia FENIB. Period: 01/2018-12/2019. Grant amount: euro 40.000. Position: principal investigator.
- Grant from Sapienza University of Rome. Characterisation of oxidative stress and mitochondrial dysfunction due to neuroserpin polymers in the dementia FENIB. Year 2017. Grant amount: euro 11.000. Position: principal investigator.
- Pilot grant from the Alpha-1 Foundation (USA). Dissecting immunomodulatory mechanisms of A1AT with function-neutralising monoclonal antibodies. Year 2017. Grant amount: $40.000. Position: principal investigator (of a network of three groups, in the UK, Spain and Italy).
- Grant from Sapienza University of Rome. Cellular pathways activated by pathological accumulation of neuroserpin polymers in the dementia FENIB. Year 2016. Grant amount: euro 15.000. Position: principal investigator.
- Grant from Sapienza University of Rome. Uncovering new toxicity pathways in the neurodegenerative dementia FENIB. Year 2015. Grant amount: euro 8.400. Position: principal investigator.
- Grant from Sapienza University of Rome. Comparative analysis of the transcriptome in cells expressing wild type or mutant neuroserpin causing dementia FENIB. Year 2014. Grant amount: euro 5.000. Position: principal investigator.
- Grant from Sapienza University of Rome. Characterisation of neurosrepin polymer toxicity in a new neuronal model of the dementia FENIB. Year 2013. Grant amount: euro 7.000. Position: principal investigator.
- Grant from the Telethon Foundation. The role of neuroserpin in familial encephalopathy with neuroserpin inclusion bodies (FENIB). Period: 11/2011 to 11/2014. Grant amount: euro 140.400. Position: co-principal investigator.
- Grant from the Pasteur Institute - Cenci Bolognetti Foundation, Rome, for researchers under 40 years of age. Handling and toxicity of polymerogenic mutants of the serpins within the endoplasmic reticulum. Period: 2011-2013. Grant amount: euro 90.000. Position: principal investigator.
- Grant from Sapienza University of Rome. Mechanisms of cell toxicity of neuroserpin polymers that cause the dementia FENIB. Year 2010. Grant amount: euro 10.000. Position: principal investigator.
- Pilot and feasibility grant from the Alpha1 Foundation. Development of monoclonal antibodies to block the polymerisation of Z a1 antitrypsin. Period: 1/7/2010 to 30/6/2011. Grant amount: $40.000. Position: co-principal investigator.
- Grant from Sapienza University of Rome. Ruolo del Reticolo Endoplasmatico in alterazioni funzionali di proteine neuronali implicate in patologie di tipo neurologico. Year 2009. Grant amount: euro 13.000. Position: principal investigator.
- MRC Programme Grant to D.A. Lomas. Pathobiology of the serpinopathies. Period: 2006-2010 (participated until 2008). Position: research associate.
- MRC Programme Grant to D.A. Lomas. Strategies to prevent cirrhosis and dementia by blocking serpin polymerisation in vivo. Period: 2000-2005. Position: research associate.
Publication metrics (source: Scopus)
ORCID ID 0000 0002 0586 8795
- ERC sector: LS3 Cellular and Developmental Biology
- H-index: 21
- Total publication number: 52
- Total citations: 2312
Publications
Articles
Miranda E, Ferrarotti I, Berardelli R, Laffranchi M, Cerea M, Gangemi F, Haq I, Ottaviani S, Lomas DA, Irving JA, Fra A (2017). The pathological Trento variant of alpha-1 antitrypsin (E75V) shows non-classical behaviour during polymerisation. FEBS Journal, 284(13):2110-2126
Guadagno NA, Miranda E (2017). Editorial: Polymer toxicity in neurodegeneration FENIB. Oncotarget, 8(22):35490-35491
Guadagno NA, Moriconi C, Licursi V, D’Acunto E, Nisi P, Carucci N, De Jaco A, Cacci E, Negri R, Lupo G, Miranda E (2017). Neuroserpin polymers cause oxidative stress in a neuronal model of the dementia FENIB. Neurobiology of Disease, 103:32-44
Motamedi-Shad N, Jagger AM, Liedtke M, Faull SV, Nanda AS, Salvadori E, Wort JL, Kay CW, Heyer-Chauhan N, Miranda E, Perez J, Ordóñez A, Haq I, Irving JA, Lomas DA (2016). An antibody that prevents serpin polymerisation acts by inducing a novel allosteric behaviour. Biochem Journal, 473(19):3269-3290
Fra A, Cosmi F, Ordoñez A, Berardelli R, Perez J, Guadagno NA, Corda L, Marciniak SJ, Lomas DA, Miranda E (2016). Polymers of Z alpha-1 antitrypsin are secreted in cell models of disease. European Respiratory Journal, 47(3):1005-1009
Saga G, Sessa F, Barbiroli A, Santambrogio C, Russo R, Sala M, Raccosta S, Martorana V, Caccia S, Noto R, Moriconi C, Miranda E, Grandori R, Manno M, Bolognesi M, Ricagno S (2016). Embelin binds to human neuroserpin and impairs its pathologic polymerisation. Scientific Reports, 6:18769
Moriconi C, Ordoñez A, Lupo G, Gooptu B, Irving JA, Noto R, Martorana V, Manno M, Timpano V, Guadagno NA, Dalton L, Marciniak S, Lomas DA, Miranda E (2015). Interactions between N-linked glycosylation and polymerisation of neuroserpin within the endoplasmic reticulum. FEBS Journal, 282(23):4565-4579
Ordóñez A, Pérez J, Tan L, Dickens JA, Motamedi-Shad N, Irving JA, Haq I, Ekeowa U, Marciniak SJ, Miranda E§, Lomas DA§ (2015). A single-chain variable fragment intrabody prevents intracellular polymerisation of Z alpha1-antitrypsin while allowing its antiproteinase activity. The FASEB Journal, 20(6):2667-2678
§Joint senior authors.
Irving JA, Miranda E, Haq I, Perez J, Kotov VR, Faull SV, Motamedi-Shad N, Lomas DA (2015). An antibody raised against a pathogenic serpin variant induces mutant-like behaviour in the wild-type protein. The Biochemical Journal, 468(1):99-108
Noto R, Randazzo L, Raccosta S, Caccia S, Moriconi C, Miranda E, Martorana V, Manno M (2015). The stability and activity of human neuroserpin are modulated by a salt bridge that stabilises the
reactive centre loop. Scientific Reports, 5:13666
Preissler S, Chambers JE, Crespillo-Casado A, Avezov E, Miranda E, Perez J, Hendershot LM, Harding HP, Ron D (2015). Physiological modulation of BiP activity by trans-promoter engagement of the interdomain linker. eLife, 4:e08961
Tan L, Perez J, Mela M, Miranda E, Burling KA, Rouhani FN, DeMeo DL, Haq I, Irving JA, Ordoñez A, Dickens JA, Brantly M, Marciniak SJ, Alexander GJM, Gooptu B, Lomas DA (2015). Characterising the association of latency with alpha1-antitrypsin polymerisation using a novel monoclonal antibody. International Journal of Biochemistry and Cell Biology, 58:81-91
Tan L, Dickens JA, Demeo DL, Miranda E, Perez J, Rashid ST, Day J, Ordoñez A, Marciniak SJ, Haq I, Barker AF, Campbell EJ, Eden E, McElvaney NG, Rennard SI, Sandhaus RA, Stocks JM, Stoller JK, Strange C, Turino G, Rouhani FN, Brantly M, Lomas DA (2014). Circulating polymers in alpha1-antitrypsin deficiency. European Respiratory Journal, 43(5):1501-1404
Ljujic M, Rankov AD, Kojic S, Miranda E, Radojkovic D (2014). Functional analysis of novel alpha-1 antitrypsin variants G320R and V321F. Molecular Biology Reports, 41(9):6133-6141
Roussel BD, Kruppa AJ, Miranda E, Crowther DC, Lomas DA, Marciniak SJ (2013). Endoplasmic reticulum dysfunction in neurological disease. The Lancet Neurology, 12(1):105-118
Ordoñez A, Snapp EL, Tan L, Miranda E, Marciniak SJ, Lomas DA (2013). Endoplasmic reticulum polymers impair luminal protein mobility and sensitise to cellular stress in alpha(1)-deficiency. Hepatology, 57(5):2049-2060
Schipanski A, Lange S, Segref A, Gutschmidt A, Lomas DA, Miranda E, Schweizer M, Hoppe T, Glatzel M (2013). A novel interaction between aging and ER overload in a protein conformational dementia. Genetics, 193(3):865-876
Lupo G, Novorol C, Smith JR, Vallier L, Miranda E, Alexander M, Biagioni S, Pedersen RA, Harris WA (2013). Multiple roles of activin/nodal, bone morphogenetic protein, fibroblast growth factor and Wnt/beta-catenin signalling in the anterior neural patterning of adherent human embryonic stem cell cultures. Open Biology, 3(4):120167
Fra AM, Gooptu B, Ferrarotti I, Miranda E, Scabini R, Ronzoni R, Benini F, Corda L, Medicina D, Luisetti M, Schiaffonati L (2012). Three new alpha1-antitrypsin deficiency variants help to define a C-terminal region regulating conformational change and polymerization. Plos One, 7(6):e38405
Schmid S, Koepke J, Dresel M, Hattesohl A, Frenzel E, Perez J, Lomas D, Miranda E, Greulich T, Noeske S, Wencker M, Teschler H, Vogelmeier C, Janciauskiene S, Koczulla A (2012). The effects of weekly augmentation therapy in patients with PiZZ α1-antitrypsin deficiency. International Journal Chronic Obstructive Pulmonary Disease, 7:687-696
Yusa K, Rashid ST, Strick-Marchand H, Varela I, Liu PQ, Paschon DE, Miranda E, Ordóñez A, Hannan NR, Rouhani FJ, Darche S, Alexander G, Marciniak SJ, Fusaki N, Hasegawa M, Holmes MC, Di Santo JP, Lomas DA, Bradley A, Vallier L (2011). Targeted gene correction of alpha1-antitrypsin deficiency in induced pluripotent stem cells. Nature, 478(7369):391-394
Morris H, Morgan MD, Wood AM, Smith SW, Ekeowa UI, Herrmann K, Holle JU, Guillevin L, Lomas DA, Perez J, Pusey CD, Salama AD, Stockley R, Wieczorek S, McKnight AJ, Maxwell AP, Miranda E, Williams J, Savage CO, Harper L (2011). ANCA-associated vasculitis is linked to carriage of the Z allele of alpha1-antitrypsin and its polymers. Annals of the Rheumatic Diseases, 70(10):1851-1856
Rodriguez-Gonzalez R, Sobrino T, Rodriguez-Yanez M, Millan M, Brea D, Miranda E, Moldes O, Perez J, Lomas DA, Leira R, Davalos A, Castillo J (2011). Association between neuroserpin and molecular markers of brain damage in patients with acute ischemic stroke. Journal of Translational Medicine, 9(1):58
Rodríguez-González R, Millán M, Sobrino T, Miranda E, Brea D, Perez de la Ossa N, Blanco M, Pérez J, Dorado L, Castellanos M, Lomas DA, Moro MA, Dávalos A, Castillo J (2011). The natural tissue plasminogen activator inhibitor neuroserpin and acute ischemic stroke outcome. Journal of Thrombosis and Haemostasis, 105(3):421-429
Chang YP; Mahadeva R, Patschull AO, Nobeli I, Ekeowa UI, McKay AR, Thalassinos K, Irving JA, Haq I, Nyon MP, Christodoulou J, Ordoñez A, Miranda E, Gooptu B (2011). Targeting serpins in high-throughput and structure-based drug design. Methods in Enzymology, 501:139-175
Irving JA, Ekeowa UI, Belorgey D, Haq I, Gooptu B, Miranda E, Perez J, Roussel BD, Ordoñez A, Dalton LE, Thomas SE, Marciniak SJ, Parfrey H, Chilvers ER, Teckman JH, Alam S, Mahadeva R, Rashid ST, Vallier L, Lomas DA (2011). The serpinopathies: studying serpin polymerization in vivo. Methods in Enzymology, 501: 421-466
Duvoix A, Miranda E, Perez J, Sorensen GL, Holmskov U, Trapnell BC, Madsen J, Clark HW, Edwards LD, Miller BE, Tal-Singer RM, Lomas DA (2011). Evaluation of full-length, cleaved and nitrosylated serum surfactant protein D as biomarkers for COPD. COPD, 8(2):79-95
Belorgey D, Irving JA, Ekeowa UI, Freeke J, Roussel BD, Miranda E, Perez J, Marciniak SJ, Crowther DC, Michel CH, Lomas DA (2011). Characterisation of serpin polymers in vitro and in vivo. Methods, 53(3):255-266
Ekeowa UI*, Freeke J*, Miranda E*, Gooptu B, Bush MF, Pérez J, Teckman J, Robinson CV and Lomas DA. Defining the mechanism of polymerization in the serpinopathies (2010). PNAS, 107(40):17146-17151
*Joint first authors
Miranda E, Perez J, Ekeowa U, Hadzic N, Kalsheker N, Gooptu B, Portmann B, Belorgey D, Hill M, Chambers S, Teckman J, Alexander G, Marciniak S, Lomas D (2010). A novel monoclonal antibody to characterize pathogenic polymers in liver disease associated with alpha1-antitrypsin deficiency. Hepatology, 52(3):1078-1088
Rashid ST, Corbineau S, Hannan N, Marciniak SJ, Miranda E, Alexander G, Huang – Doran I, Ahrlund-Richter L, Skepper J, Griffin J, Semple R, Weber A, Lomas DA, Vallier L (2010). Modelling inherited metabolic disorders of the liver with human induced pluripotent stem cells. Journal of Clinical Investigations, 120(9):3127-3136
Kröger H, Miranda E, MacLeod I, Pérez J, Crowther DC, Marciniak SJ and Lomas DA (2009). ERAD and autophagy cooperate to degrade polymerogenic mutant serpins. Journal of Biological Chemistry, 284(34):22793-22802
Davies MJ, Miranda E, Marciniak SJ, Kaufman RJ, Römisch K and Lomas DA (2009). Neuroserpin polymers activate NF-B by a calcium signalling pathway that is independent of the unfolded protein response. Journal of Biological Chemistry: 284(27):18202-18209
Medicina D, Montani N, Tiberio L, Fra AM, Corda L, Miranda E, Pezzini A, Bonetti F, Ferrarotti I, Facchetti F and Schiaffonati L (2009). Molecular characterization of the new defective Pbrescia alpha1-antitrypsin allele. Human Mutation, 30(8):E771-E781
Gooptu B, Miranda E, Nobeli I, Mallya M, Purkiss A, Leigh Brown SC, Summers C, Phillips RL, Lomas DA, Barrett TE (2009). Crystallographic and cellular characterisation of two mutations stabilising the native fold of alpha1-antitrypsin: implications for rational drug design. Journal of Molecular Biology: 387(4):857-868
Ekeowa UI, Gooptu B, Belorgey D, Hägglöf P, Karlsson-Li S, Miranda E, Pérez J, MacLeod I, Kroger H, Marciniak SJ, Crowther DC and Lomas DA (2009). Alpha1-antitrypsin deficiency, chronic obstructive pulmonary disease and the serpinopathies. Clinical Science: 116(12):837-850
Miranda E, MacLeod I, Davis MJ, Pérez J, Römisch K, Crowther D and Lomas DA (2008). The intracellular accumulation of polymeric neuroserpin explains the severity of the dementia FENIB. Human Molecular Genetics: 17(11):1527-1539
Nielsen HM, Minthon L, Londos E, Blennow K, Miranda E, Perez J, Crowther DC, Lomas DA and Janciauskiene SM (2007). Plasma and cerebrospinal fluid serpins in Alzheimer’s disease and
dementia with Lewy bodies. Neurology: 69(16):1569-1579
Kennedy S, van Diepen A, van den Hurk C, Coates LC, Lee TW, Ostrovsky LL, Miranda E, Perez J, Davies MJ, Lomas DA, Dunbar PR and Birch NP (2007). Expression of the serine protease inhibitor neuroserpin in cells of the human myeloid lineage. Thrombosis and Haemostasis: 97(3): 394-399
Miranda E and Lomas DA (2006). Neuroserpin: a serpin to think about. Cellular and Molecular Life Sciences :63(6):709-722
Hoyo-Becerra C, Lopez-Avalos MD, Perez J, Miranda E, Rojas-Rios P, Fernandez-Llebrez P, Grondona JM (2006). Continuous delivery of a monoclonal antibody against Reissner’s fiber into CSF reveals CSF-soluble material immunorelated to the subcommisssural organ in early chick embryos. Cell and Tissue Research: 326:771-786
Crowther DC, Kinghorn KJ, Miranda E, Page R, Curry JA, Duthie F, Gubb D, Lomas DA (2005). Intraneuronal A-beta, non-amyloid aggregates and neurodegeneration in a Drosophila model of Alzheimer’s disease. Neuroscience: 132:123-135
Lomas DA, Belorgey D, Mallya M, Miranda E, Kinghorn KJ, Sharp LK, Phillips RL, Page R, Robertson AS, Crowther DC (2005). Molecular mousetraps and the serpinopathies. GlaxoSmithKline Award Lecture published in Biochemical Society Transactions: 33(2):321-330
Miranda E, Romisch K, Lomas DA (2004). Mutants of neuroserpin that cause dementia accumulate as polymers within the endoplasmic reticulum. Journal of Biological Chemistry: 279(27):28283-28291
Lomas DA, Belorgey D, Mallya M, Onda M, Kinghorn KJ, Sharp LK, Phillips RL, Page R, Crowther DC, Miranda E (2004). Polymerisation underlies alpha1-antitrypsin deficiency, dementia and other serpinopathies. Review. Frontiers in Bioscience: 9:2873-2891
Crowther DC, Belorgey D, Miranda E, Kinghorn KJ, Sharp LK, Lomas DA (2004). Practical genetics: alpha-1-antitrypsin deficiency and the serpinopathies. Review. European Journal of Human Genetics: 12(3):167-172
Mann F*, Miranda E*, Weinl C*, Harmer E, Holt CE (2003). B-type Eph receptors and ephrins induce growth cone collapse through distinct intracellular pathways. Journal of Neurobiology: 57(3):323-336
*Joint first authors
Miranda E, Almonacid JA, Rodriguez S, Perez J, Hein S, Cifuentes M, Fernandez-Llebrez P, Rodriguez EM (2001). Searching for specific binding sites of the secretory glycoproteins of the subcommissural organ. Microscopy Research and Technique: 1;52(5):541-551
Fernandez-Llebrez P, Miranda E, Estivill-Torrus G, Cifuentes M, Grondona JM, Lopez-Avalos MD, Perez-Martin M, Perez J (2001). Analysis and quantification of the secretory products of the subcommissural organ by use of monoclonal antibodies. Microscopic Research Techniques: 1;52(5):510-519
Arrabal PM, Estivill-Torrus G, Miranda E, Perez J, Fernandez-Llebrez P (2000). Identification of Reissner's fiber-like glycoproteins in two species of freshwater planarians (Tricladida), by use of specific polyclonal and monoclonal antibodies. Cell and Tissue Reseach: 300(3):427-434
Estivill-Torrus G, Cifuentes M, Grondona JM, Miranda E, Bermudez-Silva FJ, Fernandez-Llebrez P, Perez J (1998). Quantification of the secretory glycoproteins of the subcommissural organ by a sensitive sandwich ELISA with a polyclonal antibody and a set of monoclonal antibodies against the bovine Reissner's fiber. Cell and Tissue Research: 294(3):407-413
Lopez-Avalos MD, Cifuentes M, Grondona JM, Miranda E, Perez J, Fernandez-Llebrez P (1997). Rostral floor plate (flexural organ) secretes glycoproteins immunologically similar to subcommissural organ glycoproteins in dogfish (Scyliorhinus canicula) embryos. Brain Research and Developmental Brain Research: 18;102(1):69-75
Book chapters
Lomas DA, Belorgey D, Miranda E, Mallya M, Hagglof P, Sharp LK, Phillips RL, Page R, Davies MJ, Crowther DC (2007). The serpinopathies and respiratory disease. In Molecular and Cellular Aspects of the serpinopathies and disorders in serpin activity. Eds. Silverman GA and Lomas DA. World Scientific, pp. 445-482
Lomas DA, Belorgey D, Mallya M, Onda M, Kinghorn KJ, Sharp LK, Phillips RL, Page R, Miranda E and Crowther DC (2004). Serpinopathies and the central nervous system. In Amyloid and Amyloidosis. Eds. G. Grateau, R. A. Kyle and M. Skinner. London, CRC Press, pp. 388-390