Biomedical Laboratory techniques

Proteomics and genetic analysis in pathological anatomy. In-situ biomarker analysis. Analytical methods on extracted DNA. In-depth analysis of different methods of DNA extraction, purification and quantification, pro and cons. Practical microscopy, observation and evaluation of samples using immunohistochemistry and FISH. Gene alterations and mutations that can be analysed for diagnostic purposes on tissue in neoplasms. Fluorescence in situ hybridisation (FISH); chromogenic in situ hybridisation (CISH); SISH. Epigenetics and methods for analysing DNA methylation. Pyrosequencing vs Sanger; Next Generation Sequencing in genes involved in response to therapy in neoplastic syndromes. Tissue Micro Array (TMA). Biological banks. Droplet Digital PCR for the identification of low-frequency point mutations in neoplasms. Identification of point mutations at the point of care (POC) using Nanopore sequencing.

F. Amaldi, P. Benedetti, G. Pesole, P. Plevani, Tecniche e metodi per la biologia molecolare, Casa Editrice Ambrosiana. Distribuzione esclusiva Zanichelli Terry A. Brown, Biotecnologie molecolari, ed. Zanichelli Molecular Diagnostic, W. B. Coleman, G. J. Tsongalis, Humana Press