Health Professions of Technical Sciences (diagnostic)

Organization of a biomedical and molecular biology laboratory. Laboratory analysis: pre-analytical, analytical, and post-analytical phases; concept of variability; quality controls; reference ranges; predictive value. Concept of Diagnostic Biomarker; examples of cardiac, stroke, and nutritional biomarkers and their detection. Analysis of renal disease biomarkers through chemical and morphological urine analysis (UCM). Basic and advanced techniques in laboratory medicine: • Flow cytometry • Impedance • Serum protein electrophoresis and immunofixation • UV-Visible spectroscopy • Radioimmunoassay, RIA • Immunofluorescence • Chromatography • Mass spectrometry • ELISA test Laboratory safety.

Knowledge of biochemistry, cell biology, structure and property of nucleic acids and proteins.

Metodologie biochimiche e biomolecolari - Strumenti e tecniche per il laboratorio del nuovo millennio. Mauro Maccarone, 2019. Editore: Zanichelli. ISBN: 9788808520555. Provided didactic material: slides presented at lectures and indication of relevant and recent scientific articles selected for each academic year.

Frontal lessons in presence and/or remote modality (for Covid-19 emergency).

Mandatory.

The learning verification will be through a written and/or oral test on topics covered in class. Knowledge of each module shall contribute to the overall assessment. To pass the exam it is necessary to achieve a grade of not less than 18/30 in each module.

Frontal lessons in presence and/or remote modality (for Covid-19 emergency).

Organization of a biomedical and molecular biology laboratory. Safety measures. Laboratory Analysis: Pre-analytical, analytical, and post-analytical phases; the concept of analytical variability; implementation of quality control measures; reference intervals; and predictive value. Concept of Diagnostic Biomarkers, with examples including cardiac biomarkers, stroke-related biomarkers, and markers of nutritional status, along with their respective detection methods. Assessment of renal disease biomarkers through chemical and morphological urine analysis (ECMU). Basic and advanced techniques in laboratory medicine: • Flow cytometry • Impedance • Serum protein electrophoresis and Immunofixation • Spectroscopy • RadioimmunoAssay, RIA • Immunofluorescence • Chromatography • ELISA

Knowledge of biochemistry, cell biology, structure and property of nucleic acids and proteins.

Metodologie biochimiche e biomolecolari - Strumenti e tecniche per il laboratorio del nuovo millennio. Mauro Maccarone, 2019. Editore: Zanichelli. ISBN: 9788808520555. Provided didactic material: slides presented at lectures and indication of relevant and recent scientific articles selected for each academic year.

Frontal lessons in presence and/or remote modality

The learning verification will be through a written and oral test on topics covered in class. Knowledge of each module shall contribute to the overall assessment. To pass the exam it is necessary to achieve a grade of not less than 18/30 in each module.