PHARMACEUTICAL AND TOXICOLOGICAL ANALYTICAL CHEMISTRY I

Course objectives

General expected learning outcomes The course aims to provide the students a theoretical and practical knowledge related to research and qualitative recognition of inorganic substances of pharmacological and toxicological interest. The aim of the course is also to provide the molecular mechanisms on the pharmaceutical and toxicological properties of the aforementioned substances. Specific expected learning outcomes 1. Knowledge and understanding The student will study all the aspects concerning the chemical reactions used for the separation and recognition of the cations included in the 6 groups of the classical systematic approach (group I: silver, lead and mercury; II group: lead, mercury, arsenic, antimony, tin, bismuth , copper, cadmium; III group: aluminium, iron, chrome, manganese; group IV: manganese, zinc, nickel, cobalt; V group: calcium, barium, strontium; group VI: magnesium, sodium, lithium, potassium) as well as anions identifiable by the research in the alkaline solution (sulphate, nitrate, chloride, bromide, iodide and oxalate). Moreover, the student will be able to apply theoretically/practically the concepts concerning the acid-base equilibria, hydrolysis, complexation, precipitation, redox reduction and be able to evaluate all the related interdependencies for the purpose of the recognition of inorganic compounds. Additionally, the student will be able to understand the molecular biological mechanisms in which cations and inorganic anions take part. 2. Applying knowledge and understanding At the end of the course, the student will be able to separate and analyse an inorganic cation or anion mixture through the application and interpretation of chemical essays. For this purpose it will be essential to know and apply the different chemical reactivity for the separation and identification of the ions (changes in pH, complexation, precipitation and redox). The practical laboratory exercises will allow the student to learn how to apply and observe what was already covered during the lectures. Knowledge of the properties and reactivity of inorganic substances will allow the student not only to understand the biological, pharmacological and toxicological functions of these substances, but also to design and / or identify possible approaches for solving relative problems. The knowledge of the most widespread therapeutic problems and pharmaceutical solutions of inorganic type (essential micronutrient elements integrators) available for the treatment of the main pathologies associated with deficiency or intoxication will make the student able to make the right decision and to give rational advice for the use of these pharmaceuticals in the field of human health. 3. Making judgements The lecturer will stimulate the students to develop a logical-critical sense by asking frequent questions (brainstorming) in class with the aim of inducing them to acquire connection skills (correlative thinking) between the various concepts defined in the syllabus leading to the mastery of the subject, connecting the chemical pharmaceutical and toxicological analysis as an integral part with a link to other disciplines already studied (general and inorganic chemistry, physics, anatomy, biology) or to be followed in the future educational path (biochemistry, physiology, pathology, pharmacology, pharmacognosy, toxicology). The lecturer will periodically ask students to develop discussions/theses related to key topics discussed in class, in order to get them used to design scientific research. At the end of the course the students will be able to formulate an analytical/critical judgment, interpret and correlate complex concepts, design a research related to the topics covered in order to expand scientific, ethical and social knowledge. 4. Communication skills Through the acquisition of knowledge and understanding, the ability to apply them and to propose a critical judgment on the topics dealt with, but also through the use of the relative scientific language used by the lecturer during the course and the frequent stimulation of the communication lesson, the student will be able to communicate with cognitive and linguistic-perceptive depth with his peers and/or with both the scientific and non-scientific community also of different cultural origin. 5. Learning skills The student who has acquired the abilities described above will be able to undertake future studies in a more autonomous, self-managed and rapid manner, but also to propose social and/or working contexts useful for the scientific progress of society in the field of human health.

Channel 1
SERGIO VALENTE Lecturers' profile
Channel 2
GIUSEPPE LA REGINA Lecturers' profile

Program - Frequency - Exams

Course program
First Part – Introduction (6 hours) 1. Contacts and office hours 2. Course features 3. Class hours 4. Exam procedure 5. Exam sessions. 6. Acquired competences 7. Exam requirements 8. Course organization 9. Laboratory practice 10. Books 11. Student evaluation. Second Part – Laboratory Safety (16 hours) 1. Laws 2. Risk of chemical agents 3. Risk prevention 4. Classification, labelling and packaging 5. Symbols 6. Material safety data sheet 7. Exposure limits 8. Individual protection devices 9. Safety signs 10. Chemical laboratory code of conduct 11. Accidental contact with a chemical agent 12. Learning verification and certification. Third Part – Basics (12 hours) 1. Chemical bonds and properties 2. Compound colours 3. Inorganic analysis 4. Laboratory operations 5. Solutions 6. Chemical equilibrium in solution 7. Acidity and basicity 8. Precipitation 9. Precipitation and pH influence 10. Oxidation and reduction 11. Oxidoreduction and pH influence 12. Oxidoreduction and precipitation. Fourth Part – Inorganic Analysis (18 hours) 1. Introduction to analysis 2. Introduction to cation analysis 3. First analytical group and insoluble residue 4. Second analytical group 5. Third analytical group 6. Fourth analytical group 7. Fifth analytical group 8. Sixth analytical group 9. Introduction to anion analysis 10. First analytical group 11. Second analytical group 12. Third analytical group 13. Simplified process for anion identification. Fifth Part – Compounds of Pharmaceutical and Toxicological Interest (4 hours) 1. Introduction 2. Cations of pharmaceutical interest 3. Anions of pharmaceutical interest 4. Compounds of pharmaceutical and toxicological interest. Sixth Part – Italian and European Pharmacopoeia (2 hours) 1. Italian Pharmacopoeia 2. European Pharmacopoeia 3. Medicamenta. Seventh Part – Laboratory Practice (60 hours) 1. Delivery of laboratory equipment 2. Flame test 3. Tube test 4. Other tests 5. Cation analysis 6. Anion analysis 7. Identification of inorganic compounds according to Pharmacopoeia 8. Identification of unknown inorganic compound and redelivery of laboratory equipment.
Prerequisites
Indispensable: knowledge of General and Inorganic Chemistry. Important: know the basic concepts of Mathematics and Physics. Useful: know the concepts of Anatomy as well as Plant and Animal Biology.
Books
Teaching and didactic materials available at https://elearning.uniroma1.it/course/view.php?id=461 A. Araneo. Chimica analitica qualitativa. 3rd Edition, 1993, Ambrosiana Milano, Italy. D. C. Harris. Fondamenti di chimica analitica quantitativa. 1st Edition, 2017, Zanichelli, Italy. D. C. Harris. Chimica analitica quantitativa. 3rd Edition, 2017, Zanichelli, Italy. Savelli F.; Bruno O. Analisi chimico-farmaceutica. 1st Edition, 2005, Piccin, Italy. D. S. Hage; J. D. Carr. Chimica analitica e analisi quantitativa. 1st Edition, 2012, Piccin, Italy. A. Peloso. Analisi chimica qualitativa inorganica. Vol I. 1st Edition, 1991, Libreria Cortina Padova, Italy. A. Peloso. Analisi chimica qualitativa inorganica. Vol II. 1st Edition, 1991, Libreria Cortina Padova, Italy. F. Manna. Analisi dei medicinali. Parte prima. 2nd Edition, 1996, CISU, Italy. F. Manna. Analisi dei medicinali. Guida pratica di laboratorio. 1st Edition, 2000, CISU, Italy. P. Barbetti; M. G. Quaglia. L’analisi qualitativa in chimica farmaceutica e tossicologica inorganica. 1st Edition, 1992, Margiacchi – Galeno, Italy. A. Gasco; F. Gualtieri; C. Melchiorre. Chimica farmaceutica. 1st Edition, 2015, Casa Editrice Ambrosiana, Italy.
Frequency
Attendance of frontal lessons is optional, but strongly recommended. Attendance of laboratory practice exercises is mandatory.
Exam mode
The assessment methods are characterized by an oral exam session set for each month of the year, excluding the month of August, and an experimental exam test concerning the laboratory practice exercises carried out immediately after the end of the course. For each call published on Infostud, lecturer is fully available to postpone the exam. The elements taken into consideration for the assessment are: knowledge of the subject in all the parts described in the exam program, use of an appropriate scientific language, active participation during the frontal lessons and laboratory exercises, ability of reasoning during the oral exam, and the ability to study independently on the texts indicated. Sufficient knowledge of the topics covered, in the various parts of the exam program, is required to pass the exam with minimum score (18/30). To achieve a score of 30/30 cum laude, the student must instead demonstrate that he/she has acquired an excellent knowledge of all the topics covered during the course, being able to connect them in a logical and consistent manner. Student must also demonstrate that he/she has mastered the matter, moving within it with security, appropriateness and simplicity.
Bibliography
Farmacopea Ufficiale Italiana. XII Edizione, 2008, Istituto Poligrafico e Zecca dello Stato. Farmacopea Ufficiale Italiana. X Edizione, 1998, Istituto Poligrafico e Zecca dello Stato. European Pharmacopoeia. 11th Edition, 2022, Council of Europe. Medicamenta. VII Edizione, 2017, Società Cooperativa Farmaceutica.
Lesson mode
The course consists of frontal lessons and individual laboratory practice exercises. Frontal lessons are given by lecturer and are interactive. Students are continuously stimulated with questions about acquired knowledge in other and/or current courses. This allows the lecturer to make evident the links between the current course and some previous courses, whose notions play a key role in understanding what is proposed in the present course. The continuous recalls/links to concepts already acquired are intended to strengthen the student's learning. Furthermore, correlations with concepts studied in previous courses accustom the student to study the proposed subject as a multidisciplinary study, leading to cognitive growth in all the disciplines foreseen by the course of study. In addition, frontal lessons consist of some specific seminars, given by academic and industrial experts. Students find all the useful material, such as slides and teaching notes, on the course website. It is important to note that slides are only a guide to the exam topics, but they can never replace the recommended texts and frontal lessons given by the lecturer. Laboratory practice exercises are individual and consist of a series of experimental experiences during which students can observe and learn methods and techniques of identification of inorganic substances by means of acquired knowledge in frontal lessons.
  • Lesson code1022761
  • Academic year2025/2026
  • CourseIndustrial pharmacy
  • CurriculumSingle curriculum
  • Year2nd year
  • Semester1st semester
  • SSDCHIM/08
  • CFU10
  • Subject areaDiscipline Farmaceutico-alimentari