Industrial pharmacy REPLICA LATINA

General expected learning outcomes This course is mainly designed to provide a deeper insight into advanced chromatographic and spectrometric techniques presented during the course of Physical Methods in Organic Chemistry (second semester of the third year). Chromatographic techniques allow the qualitative and quantitative determination of complex mixtures, obtained both from synthetic processes and from extractive processes from vegetable drugs and environmental matrices. For these reasons, such techniques play a fundamental role for a future employment in the field of regulatory affairs and healthcare industries. The students will experience the most modern techniques for the separation of complex mixtures (HPLC, UHPLC, HILIC) and the basic concepts of the coupling between liquid chromatography and mass spectrometry (LC/MS), aimed at the correct identification of the chemical structure of the studied compounds. Specific expected learning outcomes 1. Knowledge and understanding At the end of the course the student has a thorough and advanced knowledge of the chromatographic and spectroscopic methods already presented in the course of Physical Methods in Organic Chemistry. He knows the most modern techniques for the separation of complex mixtures (HPLC, UHPLC, HILIC) and the basic concepts of the coupling between liquid chromatography and mass spectrometry (LC/MS). 2. Applying knowledge and understanding At the end of the course the student knows the most widely used chromatographic materials, the theoretical principles and the most innovative chiral stationary phases for the analysis of chiral drugs; he is familiar with the concept of the inversion of the elution order of enantiomers. He knows some of the most recent applications in the field of proteomics (monolithic capillary column approach) and of stereoselective molecular recognition in the gas phase. 3. Making judgements At the end of the course the student is able to decide independently which analytical method is more useful to solve a particular trouble that he could meet in the working field, from the characterization of new active ingredients of therapeutic interest to the production and quality control of the drug. 4. Communication skills At the end of the course the student has the ability to communicate outward the knowledge he has learned during the course, both toward the scientific community and the labor market. In particular, he must be able to provide clear and direct information on the most modern chromatographic techniques and their applications in the field of drugs. 5. Learning skills The student who has passed the exam is able to continue the study by reading the most recent articles published in the literature that the teacher makes available to him on the e-learning platform. He/she is also able to check specific databases in the field.