ANALYSIS OF DRUGS AND DRUG METABOLITES IN BIOLOGICAL FLUIDS

Course objectives

General expected learning outcomes The aim of the teaching Analisi dei farmaci e dei metaboliti nei fluidi biologici is to ensure knowledge of the main aspects of the methods that can be used for the qualitative-quantitative analysis and pharmacological characterization of drugs and their metabolites, both in biological fluids and in “in vitro” model systems, from sample selection operations to processing, interpretation and transmission of results. Specific expected learning outcomes In this teaching, the students will have to acquire the following detailed skills according to Dublin descriptors: 1. Knowledge and understanding Students acquire ability to discriminate the specific purposes of the different types of analytical investigations in biological fluids. Knowledge of the characteristics, differences and specificity of the various biological matrices. Definition of criteria for the correct selection, design, validation and application of complete analytical procedures for the identification and quantitative determination of drugs and their metabolites in biological matrices. 2. Applying knowledge and understanding The student will acquire the ability to correctly frame an analytical problem, with particular regard to the research of drugs and metabolites in biological fluids of different kind and nature, and the ability to find information from the study of the reference scientific bibliography. 3. Making judgements One of the fundamental objectives of teaching is constituted by critical learning. It is pursued systematically through the continuous interaction between teacher and students. 4. Communication skills Students acquire the ability to communicate knowledge, problems and solutions related to the analysis of drugs and metabolites in biological fluids. They are also able to expose assessments and motivations in a clear and understandable way to specialists and non-specialists. 5. Learning skills The student will be able to independently learn all the analytical techniques thanks to the availability of texts and handouts and also thanks to the consultation of scientific articles related to the topics of the course, which are published in indexed international scientific journals.

Channel 1
PAOLA CHIMENTI Lecturers' profile

Program - Frequency - Exams

Course program
The analytical problem in pharmaceutical chemistry, in experimental and clinical pharmacology, in therapeutic monitoring, in toxicology and in forensic sciences. The main purposes of the analytical investigation. Analytical techniques, methods, procedures and protocols. Procedures for the identification and quantitative determination of drugs and their metabolites in biological fluids. The "analytical profile" of a drug. Criteria for the choice and development of analysis methods for the identification and quantitative determination of drugs and metabolites in biological fluids and in in vitro model systems. Screening methods and confirmation methods. Biological matrices; sample pretreatment techniques and main procedures for the extraction, purification, isolation and pre-concentration of the analytes of interest in biological fluids and in vitro model systems. Main instrumental analysis techniques. Methods for the processing and interpretation of experimental data according to the objectives of the analytical investigation. Validation and accreditation of analysis methods and test procedures. Planning a complete analytical protocol: from sampling to communication of results. Bibliographic resources: the study and consultation of the reference scientific literature and databases.
Prerequisites
To understand the lessons, notions of General Chemistry and Analytical Chemistry are indispensable, which allow a more rapid and effective understanding of the theoretical bases of instrumental analysis techniques. Knowledge of physics and mathematics are important to help in understanding the basis of analytical techniques. The knowledge of Chemical Pharmaceutical and Toxicological Analysis I, II and III and Physical Methods in Organic Chemistry are useful.
Books
The Analysis of Drugs in Biological Fluids Second Edition Joseph Chamberlain
Frequency
Participation in the course is optional but strongly recommended. In case of limitations due to the pandemic situation, the course will be held remotely or in mixed mode, using Google Meet.
Exam mode
The students' assessment method consists of an oral exam (five sessions / year plus 4 extraordinary sessions according to the needs of undergraduates or out of course students) during which the candidate is invited to present a power point on an in-depth study of the topic of his choice. inherent to the course. During the presentation, lasting no more than 20 minutes, the teacher will be able to verify the mastery of concepts and language and the consistency of the presentation; it will also verify the acquisition of notions relating to the chosen instrumental techniques and main applications, asking more specific questions relating to the topic presented. The overall duration of the final exam is estimated at around 40-45 minutes. Basic knowledge and reasoning skills on the various aspects of the course program are required to pass the exam. Excellent knowledge and skills in exposing and reworking the acquired notions are required to pass the exam with 30/30 cum laude
Lesson mode
64 hours lectures will be used to deliver the core material.
PAOLA CHIMENTI Lecturers' profile

Program - Frequency - Exams

Course program
The analytical problem in pharmaceutical chemistry, in experimental and clinical pharmacology, in therapeutic monitoring, in toxicology and in forensic sciences. The main purposes of the analytical investigation. Analytical techniques, methods, procedures and protocols. Procedures for the identification and quantitative determination of drugs and their metabolites in biological fluids. The "analytical profile" of a drug. Criteria for the choice and development of analysis methods for the identification and quantitative determination of drugs and metabolites in biological fluids and in in vitro model systems. Screening methods and confirmation methods. Biological matrices; sample pretreatment techniques and main procedures for the extraction, purification, isolation and pre-concentration of the analytes of interest in biological fluids and in vitro model systems. Main instrumental analysis techniques. Methods for the processing and interpretation of experimental data according to the objectives of the analytical investigation. Validation and accreditation of analysis methods and test procedures. Planning a complete analytical protocol: from sampling to communication of results. Bibliographic resources: the study and consultation of the reference scientific literature and databases.
Prerequisites
To understand the lessons, notions of General Chemistry and Analytical Chemistry are indispensable, which allow a more rapid and effective understanding of the theoretical bases of instrumental analysis techniques. Knowledge of physics and mathematics are important to help in understanding the basis of analytical techniques. The knowledge of Chemical Pharmaceutical and Toxicological Analysis I, II and III and Physical Methods in Organic Chemistry are useful.
Books
The Analysis of Drugs in Biological Fluids Second Edition Joseph Chamberlain
Frequency
Participation in the course is optional but strongly recommended. In case of limitations due to the pandemic situation, the course will be held remotely or in mixed mode, using Google Meet.
Exam mode
The students' assessment method consists of an oral exam (five sessions / year plus 4 extraordinary sessions according to the needs of undergraduates or out of course students) during which the candidate is invited to present a power point on an in-depth study of the topic of his choice. inherent to the course. During the presentation, lasting no more than 20 minutes, the teacher will be able to verify the mastery of concepts and language and the consistency of the presentation; it will also verify the acquisition of notions relating to the chosen instrumental techniques and main applications, asking more specific questions relating to the topic presented. The overall duration of the final exam is estimated at around 40-45 minutes. Basic knowledge and reasoning skills on the various aspects of the course program are required to pass the exam. Excellent knowledge and skills in exposing and reworking the acquired notions are required to pass the exam with 30/30 cum laude
Lesson mode
64 hours lectures will be used to deliver the core material.
DANIELA SECCI Lecturers' profile
DANIELA SECCI Lecturers' profile
  • Lesson code10612248
  • Academic year2025/2026
  • CourseIndustrial pharmacy REPLICA LATINA
  • CurriculumCurriculum unico
  • Year5th year
  • Semester1st semester
  • SSDCHIM/08
  • CFU6