THREE-DIMENSIONAL MODELING

Course objectives

General skills. The student will be able to fully describe the execution of a diagnostic test, starting from the phase of collection/preparation of biological materials, to the detection of the main macromolecules (proteins, lipids, carbohydrates) or metabolites of clinical relevance, up to the interpretation of the variations in their levels or their chemical-physical properties in the context of the physiology or pathology of different organs and tissues. Specific skills. a) knowledge and understanding: - theoretical knowledge of the main techniques used for the preparation and analysis of biological samples in biochemical diagnostics; - knowledge of the biological role of the main markers used in biochemical diagnostics; - understanding the significance of the variations in the levels and/or in the chemical-physical properties of the main markers used in biochemical diagnostics; b) applying knowledge and understanding: - ability to critically evaluate the adequacy and limits of a given experimental procedure to address specific questions in the biochemical diagnostic field; - ability to evaluate the diagnostic significance of the current and potential new markers in the context of biochemical diagnostics; c) making judgments: - ability to critically evaluate the results of the diagnostic tests presented in the course; - ability to critically analyse the scientific literature in the field of clinical biochemistry; d) communication skills: - ability to illustrate and describe the main analytical techniques with scientific rigor and properties of language; - ability to explain the results of the main diagnostic tests, linking them with the physiology and pathology of the apparatus, organ or tissue under examination; e) learning skills: - knowledge of the appropriate terminology used in the field; - acquisition of the basic knowledge to adequately support working in the biochemical diagnostics field.

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EMANUELE CACCI Lecturers' profile

Program - Frequency - Exams

Course program
- Collection and fractionation of biological materials. Centrifugation techniques. Chromatographic techniques. - Electrophoretic techniques (single and two-dimensional electrophoresis; Isoelectric focusing). Electrophoretic patterns of biological liquids and their diagnostic use (serum proteins, hemoglobins, urinary proteins). - Spectroscopic Techniques. Ultraviolet and visible spectroscopy. Spectrofluorimetry. Turbidimetry and nephelometry. Luminometry. - Determinations of enzymatic activities. Enzymatic and isoenzymatic patterns of tissues and body fluids (e.g. Diagnosis of myocardial infarction by serum isoenzyme analysis). Dosages of metabolites, evaluation of the metabolic states. Glucose determination to diagnose diabetes). Immobilized enzymes. Biosensors. - Production and use of antisera and monoclonal antibodies. Immunological techniques and their diagnostic applications (immunoprecipitation, immunoelectrophoresis tests, immunoenzymatic and radioimmunoassay methods, immunoblotting, cytofluorimetry, immunofluorescence. - Blood groups and transfusion compatibility tests.
Prerequisites
Knowledge of the basic concepts of Biochemistry, Molecular Biology and Cell Biology. Knowledge of the basic technologies used in molecular biology and biochemistry, including recombinant DNA methodologies, chromatography, electrophoresis, and enzymatic assays.
Books
The educational material for Biochemical Diagnostic consists of original scientific papers, reviews, webpages with specialized content, and book texts. Powerpoint presentations about lectures, reviews, and articles will be available on e-learning platform. Text books: Wilson and Walker's Principles and Techniques of Biochemistry and Molecular Biology Marshall, Lapsley, Day, Ayling. Clinical Biochemistry: Metabolic and clinical aspects. Churcill Livingstone, Elsevier
Frequency
class attendance is not mandatory
Exam mode
An oral interview will be used to assess student learning and knowledge
Lesson mode
The lessons will take place in the classroom, with the aid of PowerPoint slides which will subsequently be made available to the students
  • Academic year2025/2026
  • CourseBiochemistry
  • CurriculumSingle curriculum
  • Year2nd year
  • Semester1st semester
  • SSDBIO/12
  • CFU3