Medicine and Surgery

Quality control principles. General principles and applications of clinical molecular biology. Organization and automation of the molecular biology laboratory. Basic principles of molecular biology techniques for laboratory diagnostics. Nucleic acid extraction. PCR, ARMS, allelic discrimination, forward and reverse hybridization, RFLP, Southern blot, MLPA. DNA sequencing. Principles of next-generation sequencing. Functional interpretation of DNA variants. Applications of molecular diagnostics in the diagnosis, prognosis, and treatment of genetic diseases: some examples. Major cardiovascular risk factors. Laboratory diagnostics of myocardial infarction: creatine kinase, creatine kinase MB fraction, myoglobin, troponins, aspartate aminotransferase, lactate dehydrogenase. Dyslipidemias: main lipoprotein classes, receptors, and apoproteins involved in lipid metabolism. Intracellular and systemic regulation of cholesterol. Classification of dyslipidemias, Reference values ​​for cholesterol and triglycerides, Concept of cardiovascular risk, Atherosclerotic disease. Laboratory diagnosis of dyslipidemias.

Ciaccio. Trattato di Biochimica Clinica e Medicina di Laboratorio. Edizioni Edises.

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