Dentistry and Dental Prosthodontics

Module Outline: Structure and physicochemical properties of nitrogenous bases, nucleosides, and nucleotides. Nucleotide-containing cofactors and regulatory nucleotides (cAMP and cGMP). Polynucleotides. The DNA double helix: DNA A, B, and Z, differences between conformations. DNA denaturation and the hyperchromic effect. Watson, Crick, and Hoogsteen base pairing. DNA supercoiling and linking number. Enzymes that control supercoiling: topoisomerase I and II. Structure of nucleosomes and histones. RNA polymerase. Transcription factors and structural motifs in DNA-binding proteins: HTH, zinc fingers, HLH, and leucine zipper. Techniques: Enzymes used in recombinant DNA technology. DNA sequencing chemistry using the Sanger method and automated DNA sequencing. Recombinant DNA products in medicine. Microarrays. PCR and its applications in medicine.

DL Nelson & MM Cox - Lehninger Principles of Biochemistry (7th edition) D. Voet, JG Voet, CW Pratt - Fundamentals of Biochemsitry- Life at the Molecular Level (5th edition)

Module Outline: Structure and physicochemical properties of nitrogenous bases, nucleosides, and nucleotides. Nucleotide-containing cofactors and regulatory nucleotides (cAMP and cGMP). Polynucleotides. The DNA double helix: DNA A, B, and Z, differences between conformations. DNA denaturation and the hyperchromic effect. Watson, Crick, and Hoogsteen base pairing. DNA supercoiling and linking number. Enzymes that control supercoiling: topoisomerase I and II. Structure of nucleosomes and histones. RNA polymerase. Transcription factors and structural motifs in DNA-binding proteins: HTH, zinc fingers, HLH, and leucine zipper. Techniques: Enzymes used in recombinant DNA technology. DNA sequencing chemistry using the Sanger method and automated DNA sequencing. Recombinant DNA products in medicine. Microarrays. PCR and its applications in medicine.

DL Nelson & MM Cox - Lehninger Principles of Biochemistry (7th edition) D. Voet, JG Voet, CW Pratt - Fundamentals of Biochemsitry- Life at the Molecular Level (5th edition)