THREE-DIMENSIONAL MODELING

Course objectives

The course Cell biology of stem cells and applications includes two modules: : Module I Animal stem cells and applications and module II Plant cell stems and applications General skills Module II Knowledge on the basic molecular and cell properties of plant stem cells. Their genetic manipulation and use in Agricultural purpose. Specific skills A) Knowledge and understanding -knowing and understanding the nature of plant stem cells -knowing and understanding the use of stem cells in Agricultural purpose B) Applying knowledge and understanding - be able to use the specific terminology - practicing problem solving in stem cell biology C) Making judgements - critical thinking through the analysis of the main discoveries in plant stem cell biology and how it can complement reaserch in the animal stem cell field. - learning by questioning D) Communication skills -be able to communicate what has been learned during the simulation of a project research discussion E) Learning skills - learning the specific terminology - be able to make the logical connections between the topics covered - be able to identify the most relevant topics

Channel 1
RAFFAELE DELLO IOIO Lecturers' profile

Program - Frequency - Exams

Course program
Stem cells: positioning, structure, role of morphogens, similarities and differences with animals Generation of sensors for plant hormones involved in stem cell maintenance (auxin, cytokinin, and gibberellin; direct and indirect sensors based on cis-elements or FRET-FLIM approaches) Clonal analysis and stem cells (Cre/lox system, induction of the CRE protein, “Brother of Brainbow” clonal system, analysis of clonal lineages) Functional analysis of genes involved in stem cell activity and maintenance (generation of mutants and fluorescent reporters; TSKO system; transactivation systems such as UAS/GAL4 or LHGR/OP; inducible GR or dexamethasone-based systems, etc.) Stem cells and shoot apical meristem development Stem cells and root meristem development MicroRNAs and stem cell maintenance Stem cells and plant regeneration: molecular mechanisms Molecular mechanisms protecting stem cells under stress conditions
Prerequisites
Molecular Biology Developmental Biology English
Books
Plant Biology. Smith, Coupland et al, CRC press book Mechanisms in plant development, Leyser and Day Selected papers
Frequency
not obligatory
Exam mode
group presentation of a paper
Lesson mode
direct and flip flop classes
RAFFAELE DELLO IOIO Lecturers' profile

Program - Frequency - Exams

Course program
Stem cells: positioning, structure, role of morphogens, similarities and differences with animals Generation of sensors for plant hormones involved in stem cell maintenance (auxin, cytokinin, and gibberellin; direct and indirect sensors based on cis-elements or FRET-FLIM approaches) Clonal analysis and stem cells (Cre/lox system, induction of the CRE protein, “Brother of Brainbow” clonal system, analysis of clonal lineages) Functional analysis of genes involved in stem cell activity and maintenance (generation of mutants and fluorescent reporters; TSKO system; transactivation systems such as UAS/GAL4 or LHGR/OP; inducible GR or dexamethasone-based systems, etc.) Stem cells and shoot apical meristem development Stem cells and root meristem development MicroRNAs and stem cell maintenance Stem cells and plant regeneration: molecular mechanisms Molecular mechanisms protecting stem cells under stress conditions
Prerequisites
Molecular Biology Developmental Biology English
Books
Plant Biology. Smith, Coupland et al, CRC press book Mechanisms in plant development, Leyser and Day Selected papers
Frequency
not obligatory
Exam mode
group presentation of a paper
Lesson mode
direct and flip flop classes
  • Academic year2025/2026
  • CourseCell biology and technology
  • CurriculumSingle curriculum
  • Year2nd year
  • Semester1st semester
  • SSDBIO/11
  • CFU3