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Curriculum(s) for 2026 - Cell biology and technology (33595)

Single curriculum
Lesson [SSD] [Language] YearSemesterCFU
10628558 | [BIOS-15/A] [ITA]1st1st6

Educational objectives

General objectives -
The main objective of the course is to allow the student to acquire advanced knowledge of microbial genetics, including the molecular aspects, which underlie the development of many of the current techniques of molecular biology and recombinant DNA.

The educational objectives of the course are the understanding of some of the historical experiments of microbial genetics and the study of today's advances in the fields of bacterial genetics, phage and eukaryotic microbes such as the yeast Saccharomyces cerevisiae. Mutation and evolution of microbial genomes, mechanisms for regulating copy control of plasmids and chromosomes, transposons and control of transposition, mechanisms of microbial differentiation will also be topics of the course.
Specific objectives -
Knowledge and ability to understand. The specific objectives of the course include:
• Know and understand some of the historical experiments of microbial genetics, the advances in the fields of bacterial genetics, phage and eukaryotic microbes such as yeast Saccharomyces cerevisiae;
• To know and understand the role of mutations in the evolution of microbial genomes, the mechanisms of regulation of mobile elements such as plasmids and transposons and control of transposition;
General objectives -
The main objective of the course is to allow the student to acquire advanced knowledge of microbial genetics, including the molecular aspects, which underlie the development of many of the current techniques of molecular biology and recombinant DNA.

The educational objectives of the course are the understanding of some of the historical experiments of microbial genetics and the study of today's advances in the fields of bacterial genetics, phage and eukaryotic microbes such as the yeast Saccharomyces cerevisiae. Mutation and evolution of microbial genomes, mechanisms for regulating copy control of plasmids and chromosomes, transposons and control of transposition, mechanisms of microbial differentiation will also be topics of the course.
Specific objectives -
Knowledge and ability to understand. The specific objectives of the course include:
• Know and understand some of the historical experiments of microbial genetics, the advances in the fields of bacterial genetics, phage and eukaryotic microbes such as yeast Saccharomyces cerevisiae;
• To know and understand the role of mutations in the evolution of microbial genomes, the mechanisms of regulation of mobile elements such as plasmids and transposons and control of transposition.

10629473 | [BIOS-02/A] [ITA]1st1st6

Educational objectives

Obiettivi formativi

Con questo insegnamento, gli studenti acquisiranno conoscenze teoriche e metodologiche sugli approcci di studio e sulle metodologie cellulari e molecolari alla base delle biotecnologie vegetali, e sulla complessità, a livello molecolare e cellulare, delle vie metaboliche primarie e secondarie di interesse biotecnologico.

Obiettivi specifici Parte I

A) Conoscenze e capacità di comprensione

Acquisire conoscenze dettagliate sui metodi di trasformazione transiente e stabile del genoma nucleare e plastidico delle piante; sugli approcci di genetica diretta ed inversa per studiare la funzione dei geni vegetali; sull’identificazione ed utilizzo dei marcatori molecolari in genetica vegetale; sull’utilizzo delle biotecnologie vegetali per l’ottenimento di varietà coltivate con tratti agronomici migliorati. In particolare, gli studenti che abbiano superato l’esame saranno in grado di conoscere e comprendere:

- i principali sistemi di espressione transgenica stabile e transiente nelle piante, inclusi i sistemi di trasformazione dei plastidi;
- i metodi di analisi della funzione genica nelle piante tramite genetica diretta e inversa.
- i meccanismi di silenziamento e di “genome editing” negli organismi vegetali.
- l’utilizzo della variabilità genetica naturale come fonte di tratti di interesse biotecnologico ed agronomico.

B) Capacità di applicare conoscenza e comprensione

- progettare esperimenti volti a definire la funzione di un gene tramite approcci di genetica inversa;
- progettare screening genetici in sistemi modello vegetali e delineare le linee principali di identificazione di una mutazione;
- comprendere e discutere in modo critico i diversi approcci utilizzati per alterare l’espressione di un gene in una pianta e scegliere quello più opportuno in base alle esigenze ed al modello sperimentale;
- progettare l’ingegnerizzazione di nuovi tratti in organismi vegetali.

C) Autonomia di giudizio
- Capacità di giudizio critico, attraverso lo studio di rassegne e articoli scientifici su aspetti chiave del campo e approfondite discussioni ;
- Capacità di valutazione della correttezza e del rigore scientifico negli argomenti relativi alle tematiche oggetto dell’insegnamento.

D) Abilità comunicative
- Acquisizione di adeguate competenze e strumenti utili per la comunicazione in lingua italiana e in lingua straniera (inglese), mediante l'utilizzazione di linguaggi grafici e formali, con particolare riguardo al linguaggio scientifico.

E) Capacità di apprendimento
- Capacità di interpretazione ed approfondimento
- Capacità di fruizione di strumenti conoscitivi per l'aggiornamento continuo delle conoscenze
- Capacità di confronto per il consolidamento e il miglioramento delle proprie conocenze.

Obiettivi formativi specifici Parte II

A) Conoscenze e capacità di comprensione

Acquisire conoscenze dettagliate sul metabolismo primario e secondario delle piante, con particolare riferimento alla biosintesi e compartimentalizzazione dei metaboliti; sul traffico vescicolare e la secrezione; sulle basi concettuali e metodologiche dell’analisi del proteoma e del metaboloma vegetale. Gli studenti che abbiano superato l’esame saranno in grado di conoscere e comprendere (conoscenze acquisite):

- le principali vie biosintetiche nel metabolismo primario e secondario delle piante;
- i meccanismi di regolazione, channeling e compartimentalizzazione del metabolismo vegetale;
- le diverse tecnologie oggi a disposizione per l’analisi su larga scala dei proteomi e metabolomi vegetali.

B) Capacità di applicare conoscenza e comprensione

- comprendere e valutare criticamente le metodologie da utilizzare in un esperimento di proteomica o di metabolomica;
- comprendere una via metabolica vegetale e progettare la sua modifica in sistemi vegetali od in sistemi eterologhi
- affrontare la letteratura scientifica nel campo specifico, comprendendone le basi metodologiche e concettuali.

C) Autonomia di giudizio

- Capacità di giudizio critico, attraverso lo studio di rassegne e articoli scientifici su aspetti chiave del campo e approfondite discussioni ;
- Capacità di valutazione della correttezza e del rigore scientifico negli argomenti relativi alle tematiche oggetto dell’insegnamento.

D) Abilità comunicative

- Acquisizione di adeguate competenze e strumenti utili per la comunicazione in lingua italiana e in lingua straniera (inglese), mediante l'utilizzazione di linguaggi grafici e formali, con particolare riguardo al linguaggio scientifico.

E) Capacità di apprendimento

- Capacità di interpretazione ed approfondimento
- Capacità di fruizione di strumenti conoscitivi per l'aggiornamento continuo delle conoscenze
- Capacità di confronto per il consolidamento e il miglioramento delle proprie conocenze.

10628904 | [BIOS-15/A] [ITA]1st2nd6

Educational objectives

General aims

The course of Cellular Microbiology and Vaccinology aims at providing the notions that allow students to understand the complexity of the interactions of the microbial world with eukaryotic organisms. This knowledge will form the basis for a rational path that will lead them to understand the strategies underlining the formulation of vaccines. The objectives will be finalized at sequentially integrating in this schema the issues concerning the recognition of microorganisms by the innate immune system, the characteristics of the microbiota and the molecular basis of the interaction of microbiota with eukaryotic organisms. We will then proceed to the analysis of genetic and phenotypic factors that characterize the differences between commensals and pathogens and the transitional forms between these two classes. Finally, we will face the fundamentals of vaccines, their typology and composition, and the scientific and institutional process leading to their development. The prospective of the new generation vaccines and of the new vaccine targets will complete the knowledge acquired by the student. The achievement of this network of issues will provide the student with the skills to evaluate the role of microorganisms in the process of interaction with humans and the actual value of vaccines.

Specific objectives

Knowledge and understanding
• the mechanisms underlining the recognition of microorganisms by the immune system and adaptive immune responses.
• fundamental role of the microbiota in the physiological and pathological processes of eukaryotic organisms.
• the molecular bases that underlie the difference between commensals microorganism and pathogens.
• evolution of vaccines and the rationalization of the scientific and experimental path that has led to the formulation of these drugs.
• the vaccine types, the components of the vaccines and the functional roles of these.
• the key phases of vaccine development and the scientific and legislative process leading from the "bench to field"
• goals for vaccines in the near future.

Applying knowledge and understanding

• understanding the biological phenomena of complex organisms on the basis of the knowledge about microorganisms;
• interpreting the phenomena associated with microorganisms on a personal and social scale;
• perceiving the environment by integrating the key role played by microorganisms,
• feeling about themselves as a biological ecosystem and understanding the rules that govern it.

Making judgments
• Sieving information about the world of microorganisms in a conscious and reasoned way
• Making reasoned decisions about the use of products derived from bacteria and argiung about relevance of vaccines

Comunication skills
• Spreading the information acquired in the course
Communicating the issues about the vaccines on the basis of the knowledge

[N/D] [ITA]1st2nd6
[N/D] [ITA]2nd1st6
AAF1041 | STAGE [N/D, N/D] [ITA]2nd1st3

Educational objectives

The internship activities (art.10, paragraph 5, letter d - Ministerial Decree 270/04), equal to 3 credits, are dedicated to the acquisition of useful knowledge for entering the world of work. Consistent with the objectives of the degree course, these activities are aimed at applying the knowledge acquired in the fields of microbial, plant and animal cell technologies.
The internship activities include the attendance of specific working realities such as e.g. public and private research laboratories, laboratories and industries operating in the medical/health field, biotechnological industries, etc. Seminars on the development and application of innovative methodologies can also contribute to training useful for entering the world of work.

AAF1037 | FINAL EXAM [N/D] [ITA]2nd2nd39

Educational objectives

General objectives
The bibliographic study, the experimental work and the elaboration of the results for the writing of the degree thesis represent one of the most important moments of the study course of the master's degree acquisition of advanced theoretical-practical knowledge and of the correct scientific methods of investigation.
Specific objectives
Knowledge and understanding:
Knowledge and advanced scientific methodologies in specific areas of animal, plant and microbial cell biology and its applications, scientific methods of investigation, methods of collection, analysis and processing of experimental data will be acquired during the process and preparation of the final elaborate of the thesis. The thesis preparation will be carried out in university laboratories both in Italy and abroad as well as in accredited research centers and national institutes.

Ability to apply knowledge and understanding
The knowledge acquired during the preparation and discussion of the thesis allow the graduate to:
Apply knowledge for experimental design
Apply the acquired knowledge to propose advanced technologies to solve specific sector problems.

Autonomy of judgment
The development of the thesis will develop the skills of the graduates:
The autonomy of judgment in the critical evaluation of experimental data
Autonomy in the documentation and evaluation of the most appropriate experimental approaches for the solution of specific problems.

Communication skills
The preparation and discussion of the elaborate file will allow the graduate master to acquire:
-Communication skills of the research carried out and of its own global preparation.
-Ability to communicate the scientific results obtained with the thesis activity in national and international conferences
Learning ability
During the preparation and discussion of the thesis the graduate acquires the ability to learn the most advanced knowledge and methodologies and their continuous updating.