THREE-DIMENSIONAL MODELING

Course objectives

The course aims to provide students with the biochemical bases to: understand the most advanced biotechnological applications of enzymes, proteins and complex multienzymatic systems; understand the methods and strategies required for protein engineering. Students' critical and judgment skills will be developed thanks to class exercises, in which videos will be projected and numerical exercises carried out, and practical laboratory experiences, in which they will apply the concepts studied in class, performing and interpreting experiments that they will be in the future able to reproduce autonomously. Communication skills will be implemented during the theoretical lessons, which include moments of open discussion. Specific skills A) knowledge and understanding - knowledge of the main biotechnological applications of enzymes; - knowledge of the main features of complex multienzymatic systems of biotechnological interest; - knowledge of the strategies required for protein and enzyme production and engineering B) ability to apply knowledge and understanding - exploiting the knowledge of biochemical techniques to investigate the applications of enzymes and proteins in the field of biotechnology - understanding and evaluating the impact of structural modifications of biological macromolecules on their biological function; C) Making judgements - critical thinking through the study of examples of biotechnological applications of proteins and enzymes taken from the scientific literature - learning by questioning D) Communication skills - ability to communicate what has been learned during the oral exam E) Learning skills - learning the specific terminology - ability to make the logical connections between the topics covered - ability to identify the most relevant topics

Channel 1
MARIA CARMELA BONACCORSI DI PATTI Lecturers' profile

Program - Frequency - Exams

Course program
The course includes 20 hours of classroom lectures and 4 hours of guided exercises. Biotechnological applications of enzymes. Production of recombinant enzymes and proteins: methods and strategies. Protein engineering: site-specific mutagenesis, in vitro evolution. Production of therapeutic proteins (hormones, antibodies, coagulation factors). Main techniques of cell and enzyme immobilization. Biocatalysts: use of enzymes for production of biotechnologically relevant compounds. Multienzymatic systems for biosynthesis of bioactive secondary metabolites. Non-ribosomal biosynthesis of peptides. Enzyme mechanism of peptide synthetases. Examples (gramicidin, surfactin, penicillins and cefalosporins). Biosynthesis of polyketides. Mechanism of polyketide formation and analogies with fatty acid biosynthesis. Examples (erythromycin, statins).
Prerequisites
No formal propedeuticity is required. However, basic knowledge of protein biochemistry, molecular biology as well as of cellular biology is necessary.
Books
Text books and other materials are published on the educational site Elearning2 at http://elearning2.uniroma1.it
Teaching mode
Classroom lectures and guided activities.
Frequency
Lectures and guided activities are not mandatory, but attendance is strongly recommended.
Exam mode
oral presentation of a scientific article and possibly on-going tests
Lesson mode
classroom lectures and guided activities
MARIA CARMELA BONACCORSI DI PATTI Lecturers' profile

Program - Frequency - Exams

Course program
The course includes 20 hours of classroom lectures and 4 hours of guided exercises. Biotechnological applications of enzymes. Production of recombinant enzymes and proteins: methods and strategies. Protein engineering: site-specific mutagenesis, in vitro evolution. Production of therapeutic proteins (hormones, antibodies, coagulation factors). Main techniques of cell and enzyme immobilization. Biocatalysts: use of enzymes for production of biotechnologically relevant compounds. Multienzymatic systems for biosynthesis of bioactive secondary metabolites. Non-ribosomal biosynthesis of peptides. Enzyme mechanism of peptide synthetases. Examples (gramicidin, surfactin, penicillins and cefalosporins). Biosynthesis of polyketides. Mechanism of polyketide formation and analogies with fatty acid biosynthesis. Examples (erythromycin, statins).
Prerequisites
No formal propedeuticity is required. However, basic knowledge of protein biochemistry, molecular biology as well as of cellular biology is necessary.
Books
Text books and other materials are published on the educational site Elearning2 at http://elearning2.uniroma1.it
Teaching mode
Classroom lectures and guided activities.
Frequency
Lectures and guided activities are not mandatory, but attendance is strongly recommended.
Exam mode
oral presentation of a scientific article and possibly on-going tests
Lesson mode
classroom lectures and guided activities
  • Academic year2025/2026
  • CourseGenetics and Molecular Biology
  • CurriculumGenetica e Biologia Molecolare (percorso valido anche ai fini del conseguimento del doppio titolo italo-francese)
  • Year1st year
  • Semester2nd semester
  • SSDBIO/10
  • CFU3