MOLECULAR BIOLOGY FOR BIODETERIORATION: MECHANISMS AND DIAGNOSIS

Course objectives

The course is part of the activities of the English curriculum of the master's degree course in Science and Technology for the Conservation of Cultural Heritage. The general objective of the course, which includes both theoretical lectures and practical laboratory sessions, is to provide students with basic knowledge of molecular biology for the study of biodeteriorating organisms, aimed at understanding the most peculiar biodeterioration processes that affect both modern and ancient materials, and preserve and conserve them. The course provides a biomolecular approach, through the explication of concepts and principles regarding the nucleic acid sequencing techniques and the study of the metabolic activity of biodeteriorating organisms. A further objective is to understand and sustainably manage today's cultural heritage and innovative materials. The educational objectives of the course are: 1) to describe the main causes of biodeterioration in the context of cultural heritage; 2) explain the main metabolic pathways activated during the biodeterioration process; 3) describe the molecular approach associated with sequencing for the qualitative and quantitative evaluation of the biodeterioration process; 4) develop an experimental approach aimed at acquiring practical skills to study biodeterioration processes; 5) describe the main mechanisms of action of the main biocides used to control biodeterioration. A - Knowledge and understanding OF 1) To know the causes that lead to the onset of a biodeterioration process. OF 2) To understand how to modulate the environmental factors that influence the onset of the biodeterioration process. OF 3) To understand which interventions can be implemented to control biodeteriogens based on their metabolic characteristics and the environment in which they are found. B - Application skills OF 7) To be able to deduce the causes that lead to the onset of a biodeterioration process. OF 8) To be able to solve problems by modulating the environmental factors that influence the onset of the biodeterioration process OF 9) To be able to apply techniques/methods to manage the biodeterioration process. C - Autonomy of judgment OF 10) To be able to critically evaluate and discuss the results of experimental analyses aimed at identifying and characterizing biodeterioration processes considering the different engraftment matrices. OF 11) To be able to integrate the knowledge acquired in order to develop the best intervention strategy both from a methodological point of view and from an environmental impact point of view. OF 12) To be able to analyze and integrate chemical-physical-biological-environmental parameters to define the best strategy for the preservation and conservation of cultural heritage. D - Communication skills OF 13) To be able to adequately communicate the knowledge acquired using scientific language to accurately and rigorously describe the study of biodeteriogens. OF 14) To be able to interact with the teacher and with the specialized personnel who deal with the conservation and restoration of cultural heritage. E - Ability to learn OF 15) Have the ability to consult the proposed material but also to develop critical research to understand the salient aspects of the biodegradation of cultural heritage. OF 16) Have the ability to evaluate cause-effect relationships and interventions. OF 17) Have the ability to design and develop a cultural heritage conservation project.

Channel 1
MARZIA BECCACCIOLI Lecturers' profile

Program - Frequency - Exams

Course program
- Procariotic and eucariotic cell Features and differences. Plasma membrane: biochemical structure, functions; differences. Genetic heritage: DNA, genes and genomes; gene reproduction and recombination. Mitosis and meiosis. Replication. Mutations. - Biodeteriogens Bacteria: Bacterial structure, morphology and taxonomy, Bacterial DNA/RNA., Bacterial ecological niches. Archeobacteria and Cyanobacteria: morphological-structural, characteristics, ecological niches. Fungi: Fungal structure, morphology and taxonomy, Fungal DNA/RNA, Fungal ecological niches. Lichens: Morphological-structural features, symbiosis concept: functional and biochemical interactions between mycobionts and photobionts. Bryophite: Bryophite structure, morphology and taxonomy, Bryophite DNA/RNA, Bryophite ecological niches Animals: Biodeteriogenic organisms attacking the Cultural Heritage(es. mites, insects, rodents and birds) - Relationships between environmental conditions, type of substrate, nature of the colonizers. (environmental factors, substrate characteristics, biodeteriogen biodiversity) - Sampling and identification of biodeteriogens. - Engraftment and biological activity (stone, wood, paper, textile, synthetic). - Microbial metabolism - Outline of applications of molecular biology used in the preservation of cultural heritage (DNA/RNA extraction and analysis, PCR, DNA/RNA sequencing, RNAseq, gene ontology) - Conservation techniques for cultural heritage
Prerequisites
Knowledge of cell biology and microbiology is required.
Books
M.T. Madigan, J.M. Martinko, K.S. Bender, D.H. Buckley, D. A. Stahl Brock. Biology of microorganisms. Pearson Learning Solution. G. Caneva, M.P. Nugari, O. Salvadori. Plant biology for Cultural Heritage. Vol. 1: Biodeterioration and conservation. Nardini Editore.
Frequency
Theoretical lessons are not mandatory Attendance of laboratory experience is mandatory
Exam mode
The assessment of learning takes place by means of a written test, with open/close-ended questions, lasting two hours. For each question a maximum score is already established and the evaluation will be performed in a comparative manner. The objective of the exam consists in verifying and evaluating: a) the knowledge of the contents addressed in the lesson, b) the ability of critical reasoning, c) the attained ability to make a diagnosis and possible control strategies. The evaluation is expressed in thirtieths (minimum grade 18/30, maximum grade 30/30 with honors).
Lesson mode
The teaching is organized in: i) theoretical lectures, supported by slides on PowerPoint (the files in .pdf format will be downloaded by the University's Moodle e-learning platform); ii) four practical laboratory experiences: students will be involved in the study of biodeterioartion process through the use of molecular biology, they will be guided to the analysis of the results of microbial sequencig with the aim to propose the paths of biochemical deterioration. For laboratory experience there is the obligation of attendance, under penalty of failure to perform and pass the exam. iii) in-depth seminars on real cases, directly followed by the teacher, presented with the aim of showing how to develop a systemic and multidisciplinary path in the identification and management of bioterioration phenomena.
  • Lesson code10620442
  • Academic year2025/2026
  • CourseScience and Technology for the Conservation of Cultural Heritage
  • CurriculumScience and Technology for the Conservation of Cultural Heritage - in lingua inglese
  • Year1st year
  • Semester1st semester
  • SSDAGR/12
  • CFU6