CLAUDIA CARISSIMI

PROFESSORE ASSOCIATO (L. 240/10)
Università degli Studi di ROMA "La Sapienza"
Facoltà: Farmacia e Medicina
Dipartimento: Medicina molecolare

TEMATICHE DI RICERCA:
- Studio dei meccanismi molecolari e patogenici associati alle malattie infiammatorie croniche e alla base dell'insorgenza e della progressione della fibrosi intestinale.
- Identificazione, tramite approcci high-throughput, dei geni coinvolti nella patogenesi e nella risposta alla terapia
nei pazienti pediatrici affetti da Esofagite Eosinofila.
- Analisi del ruolo del fattore di trascrizione ZNF281 nello sviluppo e nella progressione del colangiocarcinoma,
un tumore dell'albero biliare epatico, e del cancro al seno.
- Studio delle peculiarità strutturali e funzionali del microbioma intestinale nelle malattie infiammatorie croniche
(IBD) e nelle patologie metaboliche (NAFLD/NASH) attraverso approcci high-throughput.

ELENCO DEI PRODOTTI DELLA RICERCA RECENTI:
Carissimi C., De Angelis F., Laudadio I., Fulci V., Stronati L., Manella S., Alvaro D.,Cardinale V. and Silecchia G. “Characterization of biliary microbiota in obese and non-obese patients with symptomatic gallstones”. IJMS, under revision (corresponding author)
Laudadio I, Leter B, Palone F, Cucchiara S, Carissimi C, Scafa N, Secci D, Vitali R and Stronati L. “Inhibition of intestinal inflammation and fibrosis by Scutellaria Baicalensis georgi and Boswellia serrata in human epithelial cells and fibroblasts” Immun Inflamm Dis. 2024 Oct;12(10), doi: 10.1002/iid3.70036
Carissimi Claudia Laudadio Ilaria, Scafa Noemi, Bastianelli Alex, Fulci Valerio, Renzini Alessandra, Russo Giusy, Oliva Salvatore, Vitali Roberta, Palone Francesca, Cucchiara Salvatore, Stronati Laura (2024). Characterization of patient- derived intestinal organoids for modelling fibrosis in Inflammatory bowel disease. INFLAMMATION RESEARCH, ISSN: 1023-3830, doi: 10.1007/s00011-024-01901-9
Graco F, Lorefice E, Carissimi C, Laudadio I, Ciccosanti F, Di Rienzo M, Colavita F, Meschi S, Maggi F, Fimia GM, Fulci V. A microRNA Arising from the Negative Strand of SARS-CoV-2 Genome Targets FOS to Reduce AP-1 Activity. Non-Coding RNA 2023, 9(3), 33; https://doi.org/10.3390/ncrna9030033

Vitali R, Mancuso AB, Palone F, Pioli C, Cesi V, Negroni A, Cucchiara S, Oliva S, Carissimi C, Laudadio I, Stronati L. PARP1 Activation Induces HMGB1 Secretion Promoting Intestinal Inflammation in Mice and Human Intestinal Organoids. Int J Mol Sci. 2023 Apr 12;24(8):7096. doi: 10.3390/ijms24087096
Vitali R, Palone F, Armuzzi A, Fulci V, Negroni A, Carissimi C, Cucchiara S,Stronati L. Proteomic Analysis Identifies Three Reliable Biomarkers ofIntestinal Inflammation in the Stools of Patients With Inflammatory BowelDisease. J Crohns Colitis. 2023 Jan 27;17(1):92-102. doi: 10.1093/ecco-cc/jjac110.
Laudadio I, Bastianelli A, Fulci V, Carissimi C, Colantoni E, Palone F,Vitali R, Lorefice E, Cucchiara S, Negroni A, Stronati L. ZNF281 Promotes Colon Fibroblast Activation in TGFβ1-Induced Gut Fibrosis. Int J Mol Sci. 2022 Sep 6;23(18):10261. doi: 10.3390/ijms231810261.
Carissimi C, Laudadio I, Lorefice E, Azzalin G, De Paolis V, Fulci V.Bisulphite miRNA-seq reveals widespread CpG and non-CpG 5-(hydroxy)methyl-Cytosine in human microRNAs. RNA Biol. 2021 Dec;18(12):2226-2235. doi:10.1080/15476286.2021.1927423. Epub 2021 Jun 7.
Putignani L, Oliva S, Isoldi S, Del Chierico F, Carissimi C, Laudadio I,Cucchiara S, Stronati L. Fecal and mucosal microbiota profiling in pediatricinflammatory bowel diseases. Eur J Gastroenterol Hepatol. 2021 Nov1;33(11):1376- 1386. doi: 10.1097/MEG.0000000000002050.
De Paolis V, Lorefice E, Orecchini E, Carissimi C, Laudadio I, Fulci V.Epitranscriptomics: A New Layer of microRNA Regulation in Cancer. Cancers(Basel). 2021 Jul 5;13(13):3372. doi: 10.3390/cancers13133372. (Corresponding author).
Fulci V, Carissimi C, Laudadio I. COVID-19 and Preparing for Future Ecological Crises: Hopes from Metagenomics in Facing Current and Future Viral Pandemic Challenges. OMICS. 2021 Jun;25(6):336-341. doi: 10.1089/omi.2021.0058.Epub 2021 May 25. (Corresponding author).
Fulci V, Stronati L, Cucchiara S, Laudadio I, Carissimi C. Emerging Roles of Gut Virome in Pediatric Diseases. Int J Mol Sci. 2021 Apr 16;22(8):4127. doi: 10.3390/ijms22084127 (last and Corresponding author)
Laudadio I, Cesi V, Carissimi C. Metagenomics in Italy and Europe: Three Actionable Challenges/Prospects in 2020. OMICS. 2020 Mar;24(3):122-123. doi: 10.1089/omi.2020.0006
Oliva S, Laudadio I, Fulci V, Rossetti D, Isoldi S, Stronati L, Carissimi C. SERPINB12 as a possible marker of steroid dependency in children with eosinophilic esophagitis: A pilot study. Dig Liver Dis. 2020
Feb;52(2):158-163. doi: 10.1016/j.dld.2019.08.018
Carissimi C, Laudadio I, Palone F, Fulci V, Cesi V, Cardona F, Alfonsi C, Cucchiara S, Isoldi S, Stronati L. Functional analysis of gut microbiota and immunoinflammation in children with autism spectrum disorders. Dig Liver Dis. 2019 Oct;51(10):1366-1374. doi: 10.1016/j.dld.2019.06.006
Laudadio I, Fulci V, Stronati L, Carissimi C. Next-Generation Metagenomics: Methodological Challenges and Opportunities. OMICS. 2019 Jul;23(7):327-333. doi: 10.1089/omi.2019.0073
Laudadio I, Carissimi C, Fulci V. How RNAi machinery enters the world of telomerase. Cell Cycle. 2019 May;18(10):1056-1067. doi: 10.1080/15384101.2019.1609834
Laudadio I, Orso F, Azzalin G, Calabrò C, Berardinelli F, Coluzzi E, Gioiosa S, Taverna D, Sgura A, Carissimi C, Fulci V. AGO2 promotes telomerase activity and interaction between the telomerase components TERT and TERC. EMBO Rep. 2019 Feb;20(2):e45969. doi: 10.15252/embr.201845969. Epub 2018 Dec 27. (co-last author).
Salvatore Oliva, Claudia Carissimi, Ilaria Laudadio, Valerio Fulci, Danilo Rossetti, Sara Isoldi, Salvatore Cucchiara, Laura Stronati (2019). Sa1295 – Serpinb12 As a Possible Marker of Steroid Dependency in Children with Eosinophilic Esophagitis: A Pilot Study. GASTROENTEROLOGY, vol. 156, p. S-309, ISSN: 0837-0036, doi: 10.1016/S0016- 5085(19)37593-6
Laudadio I, Formichetti S, Gioiosa S, Klironomos F, Rajewsky N, Macino G, Carissimi C, Fulci V. Characterization of Transcription Termination-Associated RNAs: New Insights into their Biogenesis, Tailing, and Expression in Primary Tumors. Int J Genomics. 2018 Apr 26;2018:1243858. doi:10.1155/2018/1243858. (Corresponding author).

Laudadio I, Fulci V, Palone F, Stronati L, Cucchiara S, Carissimi C. Quantitative Assessment of Shotgun Metagenomics and 16S rDNA Amplicon Sequencing in the Study of Human Gut Microbiome. OMICS. 2018
Apr;22(4):248-254. doi: 10.1089/omi.2018.0013. (last and Corresponding author)
Gioiosa S, Verduci L, Azzalin G, Carissimi C, Fulci V, Macino G. Microarray dataset of Jurkat cells following miR-93 over-expression. Data Brief. 2016 Jun 20;8:575-8. doi: 10.1016/j.dib.2016.06.001.
Carissimi C, Colombo T, Azzalin G, Cipolletta E, Laudadio I, Macino G, Fulci V. Comprehensive RNA dataset of AGO2 associated RNAs in Jurkat cells following miR-21 over-expression. Data Brief. 2016 Feb
24;7:604-6. doi: 10.1016/j.dib.2016.02.041..
Krell J, Stebbing J, Carissimi C, Dabrowska AF, de Giorgio A, Frampton AE, Harding V, Fulci V, Macino G, Colombo T, Castellano L. TP53 regulates miRNA association with AGO2 to remodel the miRNA-mRNA interaction network. Genome Res. 2016 Mar;26(3):331-41. doi: 10.1101/gr.191759.115. Epub 2015 Dec 23..
Verduci L, Azzalin G, Gioiosa S, Carissimi C, Laudadio I, Fulci V, Macino G. microRNA-181a enhances cell proliferation in acute lymphoblastic leukemia by targeting EGR1. Leuk Res. 2015 Apr;39(4):479-85. doi: 10.1016/j.leukres.2015.01.010. Epub 2015 Jan 28.
Krell J, Stebbing J, Frampton AE, Carissimi C, Harding V, De Giorgio A, Fulci V, Macino G, Colombo T, Castellano L. The role of TP53 in miRNA loading onto AGO2 and in remodelling the miRNA-mRNA interaction network. Lancet. 2015 Feb 26;385 Suppl 1:S15. doi: 10.1016/S0140-6736(15)60330-0.