Biomedical Laboratory techniques REPLICA B - ROMA AZIENDA S.CAMILLO FORLANINI

§ Origins and evolution of pathological anatomy § Acceptance, analysis and reporting of the sample § Histological diagnosis: biopsy, preparation of histological preparations, main stains of the slides, microscope observation § Cytological diagnosis: types of examination and sampling methods, preparation of cytological preparations, observation under the microscope § Electron microscopy § Immunohistochemistry § Flow cytometry § Hybridization in situ (fluorescent and non-fluorescent methods) § Tissue microarrays § Extraction and quantification of proteins § Western blotting § Extraction of nucleic acids (DNA, RNA) from fixed and paraffin-embedded tissues: methods manuals and commercials § Quantification of nucleic acids § Amplification by PCR and gel electrophoresis § Real Time PCR § Expression microarrays § Sequencing § Next Generation Sequencing § Sensitivity and specificity § Laboratory techniques and importance in the diagnosis, prognosis and therapy of the individual patient

Knowledge of biochemistry, cell biology, structure and property of DNA and proteins.

L. Ruco, A. Scarpa: Anatomia patologica- Le Basi. 2007 Edra. Didactic material presented during the lessons will be provided to students or in electronic format. Useful material will also be recommended for further study.

Mandatory.

Multiple choice quiz. Oral evaluation of the topics covered in class. It is necessary to obtain an evaluation of at least 18/30. The final grade of the teaching comes from the average of the different modules that compose it.

Frontal lessons in presence and/or remote (for Covid-19 emergency).