Biomedical Laboratory techniques REPLICA B - ROMA AZIENDA S.CAMILLO FORLANINI

Introduction to Cytopathology (2 hours) • Definition and applications of cytopathology • Difference between exfoliative cytology and fine-needle aspiration cytology • Role of the laboratory technician • Sample safety and traceability Types of Cytological Samples (2 hours) • Gynecological cytology: Pap test • Non-gynecological cytology: urine, sputum, effusions • Fine-needle aspiration cytology (FNA): thyroid, lymph nodes, breast • Methods of collection and preservation Fixation and Slide Preparation (2 hours) • Fixation techniques: physical and chemical (alcohol, spray) • Smear preparation and centrifugation techniques • Mounting and labeling of slides • Prevention of artifacts Basic Cytological Staining Methods (2 hours) • Papanicolaou stain: principles, steps, reagent preparation • May-Grünwald Giemsa (MGG) stain • Comparison of staining methods and selection based on sample type • Quality control of staining procedures Cytological Screening and Interpretation (overview) (2 hours) • Basic principles of cytological screening • Cytomorphological features of normal and pathological cells • Concept of atypia, dysplasia, and malignancy • Importance of collaboration between technician and pathologist

Robbins e Cotran. Le basi patologiche delle malattie. Edra Masson. Mottura G. Tecnica delle autopsie per riscontro diagnostico. UTET. Melis M, Carpino F, Di Tondo U. Tecniche in Anatomia Patologica. Edi-Ermes. Cardillo MR. Nozioni di tecniche diagnostiche in Anatomia Patologica. Delfino. Kiernan JK. Histological and histochemical methods. Scion Publishing Limited. last editions

Teaching materials provided during frontal lessons.