Biomedical Laboratory techniques REPLICA B - ROMA AZIENDA S.CAMILLO FORLANINI

Introduction and Laboratory Organization (2 hours) • Role of pathological anatomy in diagnosis • Sample workflow: from operating room to report • Safety, traceability, and basic regulations Tissue Fixation and Embedding (2 hours) • Types of specimens: biopsies, surgical samples, cytology • Common fixatives (formalin, alcohol) • Dehydration, clearing, and paraffin embedding • Common errors and artifacts Sectioning and Slide Preparation (2 hours) • Use of the microtome: principles and techniques • Types of blades and maintenance • Mounting samples on slides Basic Histological Staining (2 hours) • Hematoxylin and Eosin (H&E) staining • Introduction to special stains (PAS, Giemsa) • Staining quality control Introduction to Immunohistochemistry and Molecular Biology (2 hours) • Basic principles of immunohistochemistry (IHC) • Practical applications: HER2, Ki67, HPV • Overview of PCR, FISH, and NGS

Robbins e Cotran. Le basi patologiche delle malattie. Edra Masson. Mottura G. Tecnica delle autopsie per riscontro diagnostico. UTET. Melis M, Carpino F, Di Tondo U. Tecniche in Anatomia Patologica. Edi-Ermes. Cardillo MR. Nozioni di tecniche diagnostiche in Anatomia Patologica. Delfino. Kiernan JK. Histological and histochemical methods. Scion Publishing Limited. last editions

Teaching materials provided during frontal lessons.