Biomedical Laboratory techniques REPLICA B - ROMA AZIENDA S.CAMILLO FORLANINI

Generation and use of mouse models for the study of human diseases: aims, advantages, and limitations. Ethics and legislation in animal experimentation. Methodologies for the generation of "conventional" and "conditional" genetically modified mouse models: transgenic mice and "knockout" mice. The CRE/LoxP system for the generation of conditional knockout mice. Retroviral vectors for the genetic manipulation of the murine hematopoietic system. Genotyping techniques, characterization, and maintenance of colonies of genetically modified animals. Examples of mouse models for the study of human diseases: Notch and T-cell leukemias.

The teaching materials for the lessons are made available on the Sapienza platform.