Biomedical Laboratory techniques REPLICA B - ROMA AZIENDA S.CAMILLO FORLANINI

Structure and functions of the Immunohematology and Transfusion Medicine Service. Blood donor selection and collection. ABO and Rh typing; compatibility testing. Preparation and storage of blood components. Quality and safety control in transfusion medicine. Management of adverse transfusion reactions. Regulatory aspects and traceability. The role of the laboratory technician in the transfusion process. Case studies and simulations.

Basic knowledge of biology, physiology, and biochemistry. Foundations of hematology and immunohematology. General understanding of Good Laboratory Practice (GLP).

Cappellini MD, Fiorelli G. Transfusion Medicine. Edra, latest edition. Roback JD et al. AABB Technical Manual. Latest edition. Italian National Blood Centre (CNS) guidelines. Teaching materials provided by the instructor.

Attendance required for at least 75% of scheduled hours. Active participation is mandatory to achieve final eligibility.

Evaluation of active participation during the seminar. Discussion of a case study or group presentation.

nteractive lectures. Group activities and clinical case discussions. Guided visit to the transfusion medicine laboratory. Practical demonstrations of immunohematology tests.

A first part of the elective teaching activity lesson (2 hours) will be dedicated to examining the general concepts of flow cytometry: technical principles and clinical applications. A second part (3 hours) will focus on: Flow cytometry for the study of cytokines, immune system cells, circulating tumor cells, circulating stem cells. Variables affecting the analysis. Choice of filters and detectors to avoid i. The main components that characterize cytometers. Quality system in cytometry.

The student who accesses the Activity must have followed the Courses of the first and second year and have acquired the preparatory notions of Biology, Biochemistry, Anatomy and Histology. In the previous year the student must have also acquired a good study methodology and an adequate self-assessment capability.

Reference literature will be provided to students during the course

There are 5 hours of frontal teaching and 5 hours for an exercise at the MCEL laboratory.

As required by University Regulations

The learning will be verified through a written test consisting of 10 multiple-choice questions. The exam will be considered passed if the student correctly answers seven questions out of 10

the lessons are organized in face-to-face mode and if required online