Biomedical Laboratory techniques

Programma viene stabilito all'inizio del semestre rispetto la disponibilità dei docenti coinvolti

To Pass the test student must obtain a note of 18/30 for each subject of the integrated teach. Student must possess a sufficient knowledge of the program To obtain a note of 30/30 with distinction student must possess an excellent knowledge of the whole program using a correct terminology to expose the topics

Methods and instruments of investigation for the observation of cells, tissues and organs. Preparation of histological specimens. Principles of staining and reading of an histological sample. Microscopy techniques in the morphological investigation: light and electron microscopy. Immunohistochemistry and immunofluorescence on cell and tissue preparations.

Having attended the module of Histology

Lecture notes by the teacher

Classroom lectures and attendance at the optical mocroscopy laboratory

Classroom lectures and attendance at the optical mocroscopy laboratory

Practical test of recognition of a histological preparation

Lecture notes by the teacher

Classroom lectures and attendance at the optical mocroscopy laboratory

Life-cycles, pathogenesis, epidemiology, prevention, diagnosis and control of the following anthroponotic and zoonotic parasites, with particular reference to nosocomial infections due to parasitic infestations. 1- Introduction lecture of basic parasitology: life-cycle and epidemiology of human parasites. 2- Parasites in human stool (1 section). Human Gastrointestinal parasites. Protozoa, Flagellata Giardia intestinalis, Amoeba: Entamoeba histolytica, Apicomplexan protozoan: Cryptosporidium parvum/hominis; Cestodes: Taenia solium and Taenia Saginata, Diphillobothrium latum, Trematodes: Opisthorchis felineus, Clonorchis sinensis, Nematode: Trichuris trichiura and Ascaris lumbricoides, Oxyurid nematodes (pinworms) Entorobius vermicularis. 2- Parasites in human stool (2 section). Prevention of the transmission of faecal -oral parasites and diagnosis of the parasite in human stool. 3- Blood parasites (1 section): protozoan Apicomplexa malaria parasite Plasmodium falciparum/vivax/malariae/ovale, Hemoflagellates Trypanosoma cruzi (American trypanosomiasis, Chagas disease), Trypanosoma brucei (African trypanosomes), Trematodes: Schistosoma mansoni, haematobium, Nematodes lymphatic filariasis. 3- Blood parasites (2 section): Overview of the prevention, diagnosis and treatment of blood parasites. 4- Tissue parasites (1 section): Protozoan Apicomplexa Toxoplasma gondii, Flagellata Leishmaniasis, Cestodes Human echinococcosis (hydatidosis, or hydatid disease) Echinococcus granulosus/ multilocularis, Nematodes Trichinellosis and other parasite caused by the ingestion of undercooked meat/fish; parasite in vaginal and urethral secretions Trichomonas vaginalis. 4- Prevention, diagnosis, and treatment of parasite in tissue (2 section). 5- Arthropod parasites: Arachnida Ixodidae (hard ticks) and Argasidae (soft ticks); Sarcoptidae: Sarcoptes scabiei var. hominis, (Insecta Psocodea Pediculus humanus and Pthirus pubis, : Hemiptera: Cimicidae Cimex lectularius and C. hemipterus; Diptera parasite and vector (Psicodidae, Culicidae, Glossinidae, Tabanidae, myiasis).

Principle of biology, clinic medicine, prevention and hygiene. Dublin 1. Knowledge and understanding - The students are expected to acquire deep knowledge on life-cycles of main human and zoonotic parasites and their adaptation to parasitism. Moreover, they are expected to learn basic information on pathogenic mechanisms. Dublin 2. Ability to apply knowledge and understanding - The students are expected to acquire and manage the concept of “epidemiological risk” on the basis of parasite biology and clinic, and to hypothesize preventive and control strategies. Dublin 3. Critical and judgmental capacities - The students are expected to develop critical and judgmental skills by interactive discussions with the professor and with the other students purposely stimulated during the course, and will learn to connect the clinic parasitology of main human and zoonotic parasites with their epidemiology, prevention and control. Dublin 4. Ability to communicate what has been learned – Towards the end of the course, students will be requested to prepare short seminars on topics agreed with the professor and to present and discuss it with the fellow students. Dublin 5. ability to continue the study independently in the life – It is expected that at the end of the course the students are able to locate the newly acquire knowledge on human parasitology in the frame of practice aspect of clinical parasitology, as well as in the frame of preventive and control strategies, coherently with future studies.

Schede riassuntive del. Corso di Parassitologia. Prof. Vincenzo Petrarca (13 capitoli) Parassitologia e tecniche di laboratorio per le professioni sanitarie Copertina flessibile – (3 capitoli sulla parte clinica e diagnostica) di Gabriella Cancrini Editore: Lombardo Data di Pubblicazione: 2013 EAN: 9788870201321 ISBN: 8870201325

face-to face course

Students should have completed the epidemiological courses. The evaluation will be performed by oral exams, the student will demonstrate to have competence in the arguments of the course.

frontal lesson

- HPV diagnostic- - Leukocyte populations isolation from peripheral blood withdrawn - Immunophenotyping by Flow Cytometry

None

None

Based on the seminar's calendar

Not Applicable

Seminars

Structure and function of natural protein nanoparticles - Drug loading and release strategies - Techniques for evaluating nanoparticle assembly (HP-SEC, DLS, TEM) - Cellular uptake studies (flow cytometry, confocal microscopy) - Examples of the use of protein nanoparticles from scientific literature

The student must have passed the exam of Biochemical Basis of Diagnostic Sciences

Scientific articles provided by the professor

mandatory

PowerPoint presentation

The first part of the ADE will be conducted in person. The second part, which will focus on the presentation of scientific results from the literature, will be conducted remotely