Biomedical Laboratory techniques

The student must learn the principles of general and organic chemistry preliminary to the study of biochemistry, physiology and pathology. It must also learn the principles of the stoichiometric calculation essential to the initial laboratory practice, acquiring the information of Chemical Nomenclature necessary to guarantee the safety and the operativity in the Laboratory environment. The course has the intermediate propaedeutic objective the understanding and the quantitative knowledge of the laws of the gases serve a rational study of the respiratory exchanges. It also aims to understand the importance of bonds, especially the weak links essential to the study of the molecular logic of biological systems. It must understand the effect of solutes in the aqueous solvent necessary for rational learning of many biological processes. Having the knowledge of chemical nomenclature and the ability to calculate using concentration units are the essential prerequisite for a safe laboratory practice. Understanding the effect of solutes on the properties of an aqueous solution necessary for rational learning of different biological processes such as cellular exchanges and thermoregulation. To have the knowledge of the properties of chemical reactions and of the chemical equilibrium assumed to understand chemical reactivity and to understand the role of enzymes as biological catalysts. Knowing the concept of acidity and basicity and the theoretical knowledge of buffer systems is the starting point for the study of physiological pH homeostasis. The ability to control the hydrogenionic concentration during laboratory practice is an essential professional requirement for the activity of the Laboratory Technician. To know the concept of redox couples and redox potentials essential to the understanding of electronic exchanges in biological systems, for example during cellular respiration. The study of organic chemistry is aimed at understanding the importance of the role played by the Carbon atom in the construction of the main cellular constituents of the organism. The study of organic chemistry is also aimed at understanding the importance of the role played by the Carbon atom in the construction of the main cellular constituents and organism. The knowledge of the chemical reactivity properties of the main functional groups is the essential premise for the rational study of the biochemical processes that take place in the cell. The topics of the program will be carried out according to the following time line: First part: General chemistry Second part: Organic chemistry Third part: Introduction to Biochemistry https://elearning.uniroma1.it/mod/folder/view.php?id=3029

The student must have acquired the preliminary knowledge, a good study methodology and adequate self-assessment skills.

Testi di riferimento: Handbooks & Manuals: Atkins PW & Jones L Principi di Chimica Zanichelli • ISBN: 8808197883 • ISBN-13: 9788808197887 Masterton WL & Hurley CN Chimica, principi e reazioni Piccin Nuova libraria ISBN: 8829918547 ISBN 13: 9788829918546 Whitten, Davis, Peck and Stanley Chimica Piccin Nuova libraria ISBN: 978-88-299-2033-4 Brown WH & Poon T. Introduzione alla Chimica organica Edises • ISBN: 8879593277 • ISBN-13: 9788879593274 Any other modern textbooks of Chemistry is appropriate.

Lectures in the classroom using visual means.

67% attendance to be admitted to the final exam

The oral exam is held with the other teaching teachers, the final grade, expressed in thirtieths, results from the arithmetic average of the grades of the individual modules. The student should be able to discuss in general terms about the topics in program.

Not provided for

Lectures in the classroom using visual means.

Cell cultures in advanced diagnostic and therapeutic applications - Cell cultures: definition and types, culture materials and conditions, sterility and contamination - Primary cultures: isolation protocols for epithelial cells, mesenchymal cells, adult stem cells, iPS and organoid cultures - Cell culture diagnostic applications: virological, oncological and functional diagnostics - Clinical applications of cell cultures: cell therapies, in vitro reconstruction of epithelial and mucosal tissues. Examples of applications in different pathologies

No special skills are required to attend the ADE

Teaching material presented during the lessons will be provided to the students either in electronic format or as paper material. It will also be recommended useful material for any further study.

The teacher delivers lectures with traditional methods, blended methods or remote methods, according to the existing provisions, with audiovisual aids and scheduling of lessons as reported on GOMP Aule/Orari system, published on the website of the Degree Course. If possible, depending on the number of students and on the existing health provisions, part of the ADE is carried out in the laboratory, dividing the students into small groups.

Mandatory attendance

The evaluation is carried out ongoing, through questions on the topics covered and through practical laboratory exercises (where possible). For the purpose of awarding the final qualification, attendance at lessons is also taken into consideration.

The teacher delivers lectures with traditional methods, blended methods or remote methods, according to the existing provisions, with audiovisual aids and scheduling of lessons as reported on GOMP Aule/Orari system, published on the website of the Degree Course. If possible, depending on the number of students and on the existing health provisions, part of the ADE is carried out in the laboratory, dividing the students into small groups.

A first part of the elective teaching activity lesson (2 hours) will be dedicated to examining the general concepts of flow cytometry: technical principles and clinical applications. A second part (3 hours) will focus on: Flow cytometry for the study of cytokines, immune system cells, circulating tumor cells, circulating stem cells. Variables affecting the analysis. Choice of filters and detectors to avoid i. The main components that characterize cytometers. Quality system in cytometry. A third part (5 hours) During exercises at the MCEL laboratory, students can participate / assist, always under the supervision of the teacher in charge, in the analytical phase of biological samples. In particular there will be a part on the preparation of samples for reading on the instrument and a second part on data analysis and interpretation.

The student who accesses the ADE must have followed the Courses of the first and second year and have acquired the preparatory notions of Biology, Biochemistry, Anatomy and Histology. In the previous year the student must have also acquired a good study methodology and an adequate self-assessment capability.

Reference literature will be provided to students during the course

There are 5 hours of frontal teaching and 5 hours for an exercise at the MCEL laboratory.

As required by University Regulations

The learning will be verified through a written test consisting of 10 multiple-choice questions. The exam will be considered passed if the student correctly answers seven questions out of 10

the lessons are organized in face-to-face mode and if required online

Epigenetics DNA methylation Histone modifications microRNAs and non coding RNAs Study of epigenetic mechanisms Epigenetics and genetic diseases Epigenetics and cancer

The student must have acquired the basic notions, a good study methodology and adequate self-assessment skills.

Slides submitted to lessons and indication of relevant and recent scientific articles selected for each academic year.

Frontal lessons Frontal lessons in presence and/or remote (for Covid-19 emergency) focused on epigenetics and microRNAs and related laboratory methods.

Mandatory.

The learning verification will take place through a written test consisting of 10 multiple choice questions. The exam will be passed if the student correctly answers 6 out of 10 questions. Alternatively, an oral test will be carried out on topics covered during the lessons. A student's suitability judgment will be indicated.

Frontal lessons Frontal lessons in presence and/or remote (for Covid-19 emergency) focused on epigenetics and microRNAs and related laboratory methods.

Analysis by ELISA method of an endocrine hormone. Description of the prncipal functional laboratory tests in endocrinology.

Basis of endocrine system

Guida allo studio dell'endocrinologia. M. D'Armiento, A. Lenzi. Società Editrice Universo. Update guidelines on different topics.

Practice in laboratory

In presence

Questions during ADE

Practice in laboratory